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  4. FURIN Recombinant Monoclonal Antibody

FURIN Recombinant Monoclonal Antibody

  • 货号：
    CSB-RA271669A0HU
  • 规格：
    ￥1320
  • 图片：
    • Western Blot
      Positive WB detected in: A549 whole cell lysate, Hela whole cell lysate, A375 whole cell lysate, HT-29 whole cell lysate, HepG2 whole cell lysate, U-87 whole cell lysate
      All lanes: Furin antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 87 kDa
      Observed band size: 87, 72 kDa
    • IHC image of CSB-RA271669A0HU diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
  • 其他：

产品详情

  • 产品描述：

    FURIN is a subtilisin-like proprotein convertase with diverse cellular functions. Furin has a well-known role in viral pathogenesis and efficiently cleaves polybasic or multi-basic sites such as those found in influenza virus subtype H5 and H7. Emerging evidence suggests that FURIN plays a critical role in the activation and infectivity of SARS-CoV-2. In SARS-CoV-2, structural studies have suggested that furin cleavage at the S1/S2 boundary primes the spike for an open conformation required for binding to the ACE2 entry receptor.

    The generation of this recombinant FURIN antibody occurs in a series of steps: immunization, splenocytes & PBMC, single B cell sorting, mRNA extraction, RT-PCR & insert vector, expression, ELISA validation. And ELISA, WB, IHC was carried out Every step was performed under strict standards to ensure the researchers can have a recombinant FURIN antibody with premium quality.

     

  • Uniprot No.：
    P09958
  • 基因名：
    FURIN
  • 别名：
    FURIN; FUR; PACE; PCSK3; Furin; Dibasic-processing enzyme; Paired basic amino acid residue-cleaving enzyme; PACE
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide derived from human Furin
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    1C3
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Ubiquitous endoprotease within constitutive secretory pathways capable of cleavage at the RX(K/R)R consensus motif. Mediates processing of TGFB1, an essential step in TGF-beta-1 activation. Converts through proteolytic cleavage the non-functional Brain natriuretic factor prohormone into its active hormone BNP(1-32).; (Microbial infection) Cleaves and activates diphtheria toxin DT.; (Microbial infection) Cleaves and activates anthrax toxin protective antigen (PA).; (Microbial infection) Required for H7N1 and H5N1 influenza virus infection probably by cleaving hemagglutinin.; (Microbial infection) Able to cleave S.pneumoniae serine-rich repeat protein PsrP.; (Microbial infection) Facilitates human coronaviruses EMC and SARS-CoV-2 infections by proteolytically cleaving the spike protein at the monobasic S1/S2 cleavage site. This cleavage is essential for spike protein-mediated cell-cell fusion and entry into human lung cells.; (Microbial infection) Facilitates mumps virus infection by proteolytically cleaving the viral fusion protein F.
  • 基因功能参考文献：
    1. High FURIN levels show signs of lower disease activity suggesting that FURIN might have a protective role in primary Sjogren's syndrome. PMID: 29465367
    2. the furin inhibitor dec-RVKR-CMK blocked entry of MERS-CoV harboring an S protein lacking furin cleavage sites; it even blocked entry into furin-deficient LoVo cells. In addition, dec-RVKR-CMK inhibited not only the enzymatic activity of furin but also those of cathepsin L, cathepsin B, trypsin, papain, and TMPRSS2. PMID: 30021905
    3. Using protein X-ray crystallography, this study investigated the extended substrate binding site of furin in complex with three peptide-derived inhibitors at up to 1.9 A resolution. PMID: 29314830
    4. In squamous cell carcinoma of the cervix (SCCC), higher levels of MMP-14 expression were established in tumor cells, as evidenced by IHC (+3) and RT-PCR.Furin activity in the tumor was much higher than that in normal tissues. The expression of TIMP-2 mRNA was sufficiently obvious in both the tumor and normal tissues to the bottom of the uterine cavity. PMID: 29265076
    5. these findings identify furin as an important factor for aRMS progression and survival. Thus, we propose furin as a novel therapeutic target for treatment of aRMS. PMID: 27572312
    6. Novel protein crystallographic structures of the prototypical PC family member furin in different functional states were determined to 1.8-2.0 A. PMID: 27647913
    7. findings uncover a role of ERK1 in the regulation of furin activity by supporting a self-sustaining loop for high TGF-beta activity in glioma-initiating cells. PMID: 28484053
    8. Divers studies have confirmed the expression of furin in a large spectrum of cancers. Functional studies modulating its activity uncovered its importance for the processing of many cancer-related substrates and strongly indicate that high furin activity promotes the malignant phenotype of cancer cells. [review] PMID: 28369813
    9. Furin plays an important role in the malignant phenotype of rhabdomyosarcoma cells by activating proteins involved in tumor growth, vascularization, metastasis and invasion. PMID: 27548722
    10. Furin promotes epithelial-mesenchymal transition in pancreatic cancer cells probably via Hippo-YAP pathway and may be a potential target for anti-pancreatic cancer. PMID: 28259973
    11. Results suggest that (pro)renin receptor (s(P)RR) is generated by sequential processing by site-1 protease (S1P) and furin protein. PMID: 28013223
    12. Compared with the homozygous genotype CC of rs2071410, the frequency of CG + GG genotype in the case group was significantly higher than in the control group. Individuals with CG + GG genotype had 49.3% increased risk of Transient Ischemic Attack compared with individuals with CC genotype. Patients with CG + GG genotype had worse 90-day prognosis after Transient Ischemic Attack treatment than patients with CC genotype. PMID: 27760099
    13. our study provides evidence that furin activity and epithelial-mesenchymal transition are potential indicators of the aggressive phenotype in recurrent laryngeal cancer after irradiation and that furin is a potentially useful target against laryngeal cancer PMID: 27798871
    14. The s show that Human papillomavirus type 16 L2 is cleaved during virion morphogenesis in differentiated tissue and that tissue-derived native virus initiates infection independent of cellular furin. PMID: 26569287
    15. Data suggest that the full Crimean-Congo hemorrhagic fever virus can be reproduced by reverse genetics; in this system, furin cleavage of the nonstructural glycoprotein reveals mature GP38. PMID: 25933376
    16. we could not confirm an effect of the SNP on FURIN expression in vitro and no correlations could be found in vivo with FURIN expression or outcome. PMID: 26137475
    17. the plasma level of FURIN does not predict the severity of infectious disease, it may be of use in the diagnostics of autoimmune diseases. PMID: 26346780
    18. These data highlight a positive-feedback loop whereby Notch1-dependent furin expression can induce Notch1 signaling by increasing Notch1 processing and by potentiating the activity of ADAM10 and Membrane Type 1 Matrix Metalloproteinase. PMID: 26283728
    19. Furin and PC7 siRNAs induced HIF-1alpha protein by increasing its translation, resulting in upregulation of VEGF-A. PMID: 24436242
    20. High levels of furin, TNF-alpha and TGF-beta2 may be the reason of proceeding decidualization, placentation, and prevention from abortion, in spite of terminating the fetal life. PMID: 26065233
    21. a phenotypic analysis demonstrated that a novel cis-eQTL SNP rs4702 for FURIN is strongly associated with both diastolic (p = 0.012) and systolic (p = 0.035) BP levels, as well as peripheral vascular resistance (p = 0.003). PMID: 25813623
    22. FUR transcription is activated by CREB-dependent stimulation of the FUR P1 promoter during human trophoblast syncytialization. PMID: 25175744
    23. Hepatic overexpression of human profurin reduced atherosclerotic lesion development in Ldlr(-/-)mice and reduced plasma LDL-c. In the presence of profurin, hepatic ApoB, mainly ApoB100, was degraded by proteasomes. PMID: 25026302
    24. Up-regulation of MT1-MMP and furin expression and low TIMP-2 expression make the main contribution to the invasiveness potential of squamous cell cervical carcinomas. PMID: 25552509
    25. These data support a model in which HD5 prevents furin from accessing human papillomavirus 16 L2 by occluding the furin cleavage site via direct binding to the viral capsid. PMID: 25540379
    26. a central regulator of cell-mediated immunity and Th1/2 cell balance and peripheral immune toleranc. PMID: 25355923
    27. we show that the K153R mutation significantly increases the rate of proteolysis of promyostatin by furin, but has no effect on the activity of the latent complex or the cleavage of the latent complex by bone morphogenetic protein 1 (BMP-1). PMID: 25543063
    28. The study presents a novel preparation of human furin and the first X-ray crystal structures of this enzyme in complex with noncovalent inhibitors. PMID: 24666235
    29. furin appears to be the essential enzyme for the generation of kisspeptins. PMID: 24454770
    30. High FURIN expression is associated with lung cancer. PMID: 23065687
    31. H9N2 HA can be cleaved in cells with high levels of furin expression. PMID: 24257604
    32. overexpression of furin can have either favourable or detrimental effects, it seems advisable to take indirect proprotein convertase inhibitory activity into account when polyphenols are considered for therapy of hepatocellular carcinoma. PMID: 23835774
    33. the role of serpinB8/furin in obesity-associated chronic inflammation PMID: 23936445
    34. Data indicate that alpha1PDX-serpin B8 and furin-PC chimeras suggeted new serpin and protease (re)active-site and exosite determinants of reactivity. PMID: 23744066
    35. The furin function is required for the development of syncytiotrophoblast structure in the labyrinth layer, as well as for normal embryonic development. PMID: 23598405
    36. The genetic variation of furin is not associated with insulin resistance in this Chinese Kazakh cohort. PMID: 23302673
    37. overexpression of PCs, furin and PC5, but not PC7, which are all expressed in SMC, increase PKGI cleavage in a dose-dependent manner PMID: 23686857
    38. His-69 functions as a pH sensor that regulates compartment-specific furin activation PMID: 23653353
    39. Among the PC family members, only furin activates hepcidin in hepatocytes, and uniquely the full-length membrane-bound PC7 can directly shed hTfR1 by cleavage at Arg100 PMID: 23390091
    40. Endothelial protease nexin-1 is a novel regulator of A disintegrin and metalloproteinase 17 maturation and endothelial protein C receptor shedding via furin inhibition. PMID: 23661674
    41. Genetic variations of Furin gene are not associated with obesity in Kazakh general population. PMID: 23568742
    42. Liver endothelial differentiation-associated protein (LEDA-1/PIANP) is processed by a furin-like proprotein convertase. PMID: 23558288
    43. Furin-cleaved proprotein convertase subtilisin/kexin type 9 (PCSK9) is active and modulates low density lipoprotein receptor and serum cholesterol levels. PMID: 23135270
    44. Cellular furin abundance in five cell lines was shown to be strongly correlated with productive IBV infection. Cleavage of IBV spike protein by furin may contribute to the productive IBV infection in these cells. PMID: 22995191
    45. Furin overexpression inhibited hepatocellular carcinoma tumor growth. PMID: 22808247
    46. expression of furin in the basal layer of the epidermis increased tumor development and enhanced tumor growth PMID: 22577343
    47. the association between Furin and its substrate MT1-MMP is regulated by PDGFR stimulation and c-Src. PMID: 22038627
    48. Furin-resistant Sema3E inhibits endothelial cells and hampers tumour angiogenesis and tumour growth. PMID: 22247010
    49. FURIN and its substrate cytokines BAFF and APRIL are over expressed in atherosclerotic plaques. PMID: 21889147
    50. These observations support the notion that hypoxia promotes the formation of a peripheral processing compartment where furin translocates for enhanced processing of proproteins involved in tumorigenesis. PMID: 21503879

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  • 亚细胞定位：
    Golgi apparatus, trans-Golgi network membrane; Single-pass type I membrane protein. Cell membrane; Single-pass type I membrane protein. Secreted. Endosome membrane; Single-pass type I membrane protein.
  • 蛋白家族：
    Peptidase S8 family, Furin subfamily
  • 组织特异性：
    Seems to be expressed ubiquitously.
  • 数据库链接：

    HGNC: 8568

    OMIM: 136950

    KEGG: hsa:5045

    STRING: 9606.ENSP00000268171

    UniGene: Hs.513153