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METAP2 Recombinant Monoclonal Antibody

  • 货号：
    CSB-RA294332A0HU
  • 规格：
    ￥1320
  • 图片：
    • IHC image of CSB-RA294332A0HU diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
  • 其他：

产品详情

  • 产品描述：

    METAP2 is an enzyme responsible for the cleavage of the initiator methionine from nascent peptides during translation. In addition to regulating post-translational processing and protein synthesis, METAP2 also plays an important role in the development of different types of cancer. Overexpression of METAP2 has been detected in many forms of cancer including human colon cancer, malignant lymphomas, and esophageal squamous carcinomas. Inhibition of METAP2 has been shown to block angiogenesis and suppress tumor growth in preclinical tumor models.

    Compared with the polyclonal and monoclonal antibodies of METAP2, this METAP2 recombinant antibody has the features of increased reproducibility and control, animal-free technology, high degree of monovalency, high batch-to-batch consistency, easier isotype conversion, etc. And it has been validated in ELISA, IHC.

  • Uniprot No.：
    P50579
  • 基因名：
  • 别名：
    Methionine aminopeptidase 2 (MAP 2) (MetAP 2) (EC 3.4.11.18) (Initiation factor 2-associated 67 kDa glycoprotein) (p67) (p67eIF2) (Peptidase M), METAP2, MNPEP P67EIF2
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide derived from human Methionine Aminopeptidase 2
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    8F2
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, IHC
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). The catalytic activity of human METAP2 toward Met-Val peptides is consistently two orders of magnitude higher than that of METAP1, suggesting that it is responsible for processing proteins containing N-terminal Met-Val and Met-Thr sequences in vivo.; Protects eukaryotic initiation factor EIF2S1 from translation-inhibiting phosphorylation by inhibitory kinases such as EIF2AK2/PKR and EIF2AK1/HCR. Plays a critical role in the regulation of protein synthesis.
  • 基因功能参考文献：
    1. MetAP1 and MetAP2 have roles in driving cell selectivity for a potent anti-cancer agent in synergy, by controlling glutathione redox state PMID: 27542228
    2. Suggest MetAP2 as important regulator of proliferation/apoptosis in non-small cell lung cancers. PMID: 26935506
    3. Nt-acetylation prevents the excision of the initiator methionine by MetAP2. PMID: 25886145
    4. Data indicate that methionine aminopeptidase 2 (MetAP2) contains a single disulfide bond that exists in oxidized and reduced states and influences enzyme function. PMID: 24700462
    5. The activity of unmodified, nitrated and oxidised METAP2 was assessed and it was found that nitration significantly reduced its ability to cleave a chromogenic substrate. Mass spectrometry analysis identified Tyr336 as a nitrated residue in METAP2. PMID: 24041691
    6. Increased expression of METAP2 is associated with pilocytic astrocytoma. PMID: 23161775
    7. This study demonstrated a hitherto-undescribed role of MetAP2 in definitive hematopoiesis and a possible link to noncanonical Wnt and ERK signaling. PMID: 21937698
    8. the substrate specificities of Escherichia coli MetAP1, human MetAP1, and human MetAP2 were systematically profiled PMID: 20521764
    9. Data indicate that MetAP2 expression and pp60c-src phosphorylation were decreased in NC2213 treated cells. PMID: 19703310
    10. the functional role of S100A4 in regulating endothelial cell growth and tumor metastasis involves interaction with the N-terminal half of Methionine Aminopeptidase 2. PMID: 11994292
    11. High expression in germinal center B cells and their neoplastic counterparts. PMID: 12118091
    12. Divalent metal cofactors are physiologically relevant for activity of this enzyme. PMID: 12718546
    13. MetAP protein has a role in colorectal adenocarcinoma progression PMID: 15102683
    14. Expressed in colon cancer cells PMID: 15336565
    15. MetAP-1 and MetAP-2 have essential functions in the control of mammalian cell proliferation PMID: 15962312
    16. A comparison of the structual differences between Type I and Type II methionine aminopeptidases. PMID: 16274222
    17. Data suggest that methionine aminopeptidase 2 plays a role in the proliferation of fibroblasts and myofibroblasts in fibrotic lung diseases and may serve as a novel pharmacologic target in idiopathic pulmonary fibrosis. PMID: 17446530
    18. MetAP2 plays an important role in tumor cell growth and may contribute to tumorigenesis PMID: 18264137
    19. Discovery, identification, and characterization of candidate pharmacodynamic markers of METAP2 inhibition are reported. PMID: 18828628
    20. Using bovine cell extract, fumagillin was found to be bound by methionine aminopeptidase (MetAP-2). PMID: 9177176

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  • 亚细胞定位：
    Cytoplasm. Note=About 30% of expressed METAP2 associates with polysomes.
  • 蛋白家族：
    Peptidase M24A family, Methionine aminopeptidase eukaryotic type 2 subfamily
  • 数据库链接：

    HGNC: 16672

    OMIM: 601870

    KEGG: hsa:10988

    STRING: 9606.ENSP00000325312

    UniGene: Hs.444986