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SYNCRIP Recombinant Monoclonal Antibody

  • 货号：
    CSB-RA984434A0HU
  • 规格：
    ￥1320
  • 图片：
    • IHC image of CSB-RA984434A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
    • IHC image of CSB-RA984434A0HU diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
    • Immunofluorescence staining of Hela Cells with CSB-RA984434A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
  • 其他：

产品详情

  • 产品描述：

    SYNCRIP, also known as hnRNP Q or NSAP1)0, is an evolutionarily conserved RBP across eukaryotic organisms and participates in regulating various aspects of neuronal development and plasticity. It plays important role in neuronal, myeloid leukemia stem cell, and muscular development. Abnormal expression of SYNCRIP is associated with immune response disorders and neurodegenerative disorders. On the RNA side, SYNCRIP has multiple roles in the control of RNA metabolism through recognizing a variety of sequences and regulating pre-mRNA splicing, translation, transport as well as degradation.

    The main steps in the production of this SYNCRIP recombinant antibody include immunization; harvest of positive spleen cells; obtaining the antibody sequence by screening and sequencing; expression of the target antibody in mammalian cells; purification. The SYNCRIP antibody was produced recombinantly and has many advantages: high reproducibility, specificity and scalability. And has been validated in ELISA, IHC, IF.

  • Uniprot No.：
    O60506
  • 基因名：
    SYNCRIP
  • 别名：
    Heterogeneous nuclear ribonucleoprotein Q (hnRNP Q) (Glycine- and tyrosine-rich RNA-binding protein) (GRY-RBP) (NS1-associated protein 1) (Synaptotagmin-binding, cytoplasmic RNA-interacting protein), SYNCRIP, HNRPQ NSAP1
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide derived from human hnRNP Q
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    9C7
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:50-1:200
    IF 1:20-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Heterogenous nuclear ribonucleoprotein (hnRNP) implicated in mRNA processing mechanisms. Component of the CRD-mediated complex that promotes MYC mRNA stability. Isoform 1, isoform 2 and isoform 3 are associated in vitro with pre-mRNA, splicing intermediates and mature mRNA protein complexes. Isoform 1 binds to apoB mRNA AU-rich sequences. Isoform 1 is part of the APOB mRNA editosome complex and may modulate the postranscriptional C to U RNA-editing of the APOB mRNA through either by binding to A1CF (APOBEC1 complementation factor), to APOBEC1 or to RNA itself. May be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. Interacts in vitro preferentially with poly(A) and poly(U) RNA sequences. Isoform 3 may be involved in cytoplasmic vesicle-based mRNA transport through interaction with synaptotagmins. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma activation assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation; seems not to be essential for GAIT complex function.
  • 基因功能参考文献：
    1. A cryptic RNA-binding domain mediates Syncrip recognition and exosomal partitioning of miRNA targets. PMID: 29483512
    2. our data indicate that hnRNP Q1 is a novel trans-acting factor that binds to Aurora-A mRNA 5'-UTRs and regulates its translation, which increases cell proliferation and contributes to tumorigenesis in colorectal cancer PMID: 28079881
    3. SYNCRIP is required for survival of leukemia cells. SYNCRIP controls the myeloid leukemia stem cell program. PMID: 28436985
    4. The acidic domain is a unique structural feature of the splicing factor SYNCRIP. PMID: 27081926
    5. hnRNPQ6 is required for APOBEC1-enhanced IL8 production. PMID: 25100733
    6. The hnRNP Q is a novel substrate of SHP2 and the SHP2 activity may be also involved in RNA metabolisms via dephosphorylation of hnRNP Q. PMID: 23946508
    7. The inhibitory effect of hnRNP Q on YB-1 mRNA translation can be explained by its ability to pro-mote YB-1 binding to the regulatory element within the YB-1 mRNA 3' UTR. PMID: 23980891
    8. These data indicate that hnRNP Q can stimulate the protein production of HIV-1 Rev-dependent mRNAs without changing mRNA levels and mRNA export, respectively. PMID: 23679954
    9. Data demonstrate that expression levels of hnRNP A1, Q, K, R, and U influence HIV-1 production by persistently infected PMID: 19808671
    10. The overexpression of NSAP1 specifically enhanced HCV IRES-dependent translation, and knockdown of NSAP1 by use of a small interfering RNA specifically inhibited the translation of HCV mRNA. PMID: 15340051
    11. SYNCRIP is transported within the dendrite as a component of mRNA granules PMID: 15475564
    12. methylation of hnRNPQ is important for its nuclear localization PMID: 16765914
    13. Results suggest hnRNPQ interacts via its acidic domain (AcD) with Apobec1 and that this interaction is regulated by AcD phosphorylation. PMID: 17010310
    14. Data demonstrate that hnRNP Q is a splicing modulator of SMN2, further underscoring the potential of hnRNP Q as a therapeutic target for spinal muscular atrophy. PMID: 18794368
    15. Results demonstrate that galectin-3 stabilizes hnRNP Q via complex formation, and reduction in the hnRNP Q level leads to slow proliferation and less susceptibility to 5-FU. PMID: 19137262
    16. SYNCRIP participates in both RNA replication and translation in HCV life cycle. PMID: 19232660

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  • 亚细胞定位：
    Cytoplasm. Microsome. Endoplasmic reticulum. Nucleus.; [Isoform 1]: Nucleus, nucleoplasm.; [Isoform 2]: Nucleus, nucleoplasm.; [Isoform 3]: Nucleus, nucleoplasm.
  • 组织特异性：
    Ubiquitously expressed. Detected in heart, brain, pancreas, placenta, spleen, lung, liver, skeletal muscle, kidney, thymus, prostate, uterus, small intestine, colon, peripheral blood and testis.
  • 数据库链接：

    HGNC: 16918

    KEGG: hsa:10492

    STRING: 9606.ENSP00000358635

    UniGene: Hs.571177