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  4. Recombinant Arabidopsis thaliana Double-stranded RNA-binding protein 4 (DBR4)

Recombinant Arabidopsis thaliana Double-stranded RNA-binding protein 4 (DBR4)

  • 中文名称：
    Recombinant Arabidopsis thaliana Double-stranded RNA-binding protein 4(DBR4),Yeast
  • 货号：
    CSB-YP818138DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Arabidopsis thaliana Double-stranded RNA-binding protein 4(DBR4),Yeast
  • 货号：
    CSB-EP818138DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Arabidopsis thaliana Double-stranded RNA-binding protein 4(DBR4),Yeast
  • 货号：
    CSB-EP818138DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Arabidopsis thaliana Double-stranded RNA-binding protein 4(DBR4),Yeast
  • 货号：
    CSB-BP818138DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Arabidopsis thaliana Double-stranded RNA-binding protein 4(DBR4),Yeast
  • 货号：
    CSB-MP818138DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    DBR4
  • Uniprot No.：
  • 别名：
    DBR4; At3g62800; F26K9.230Double-stranded RNA-binding protein 4; dsRNA-binding protein 4; AtDRB4
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-355
  • 氨基酸序列
    MDHVYKGQLQ AYALQHNLEL PVYANEREGP PHAPRFRCNV TFCGQTFQSS EFFPTLKSAE HAAAKIAVAS LTPQSPEGID VAYKNLLQEI AQKESSLLPF YATATSGPSH APTFTSTVEF AGKVFSGEEA KTKKLAEMSA AKVAFMSIKN GNSNQTGSPT LPSERQEDVN SNVKSSPQEI HSQPSSKVVM TPDTPSKGIK VNEDEFPDLH DAPASNAKEI NVALNEPENP TNDGTLSALT TDGMKMNIAS SSLPIPHNPT NVITLNAPAA NGIKRNIAAC SSWMPQNPTN DGSETSSCVV DESEKKKLIM GTGHLSIPTG QHVVCRPWNP EITLPQDAEM LFRDDKFIAY RLVKP
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Double-stranded RNA-binding protein involved in RNA-mediated post-transcriptional gene silencing (PTGS). Functions in the trans-acting small interfering RNAs (ta-siRNAs) biogenesis by binding and assisting DICER-LIKE 4 (DCL4). Required for DCL4 activity. Required for the 21 nucleotide ta-siRNAs production of the TAS3 transcript in leaves but not in flowers. Plays an important role in silencing RNA of both DNA and RNA viruses. Involved with argonaute 7 (AGO7) and RDR6 in turnip crinkle virus (TCV) silencing. May not be directly involved in viral siRNA production. May stabilize the 21 nucleotide viral siRNAs and deliver them to the RISC complex. Targeted by the viral silencing suppressor (VSR) transactivator/viroplasmin (TAV) protein of the cauliflower mosaic virus (CaMV) that inactivates DRB4 function in RNA silencing. Probably not involved in the guide strand selection from RNA duplexes. Involved in leaf morphology through its function in ta-siRNA-mediated silencing.
  • 基因功能参考文献：
    1. High DRB4 expression is associated with defense against turnip crinkle virus. PMID: 29513740
    2. Data indicate a potential role of the C-terminal region of double-stranded-RNA-binding protein 4 (DRB4) in protein:protein interaction as it possesses six PxxP motifs, binds to Zn2+ and contains a small structural domain. PMID: 28575480
    3. The DRB7.1-DRB4 complex can antagonize RNase III activity and the production of siRNAs in plants. PMID: 28232389
    4. Turnip crinkle virus infection promotes the cytosolic accumulation of the otherwise primarily nuclear DRB4, and this in turn inhibits HRT-DRB4 interaction. PMID: 24614040
    5. DCL4 and its interacting partner dsRNA-binding protein 4 (DRB4) participate in the antiviral response to Turnip yellow mosaic virus (TYMV), and that both proteins are required for TYMV-derived small RNA production. PMID: 21883552
    6. DRB4 is specifically required for DCL4 activity in vitro. dsRNA-binding domains are critical for DRB4 function. PMID: 21270136
    7. a fraction of P6 immunoprecipitates with DRB4 in CaMV-infected cells (viral P6). PMID: 18615098
    8. implicate DRB4 in antiviral RNA silencing PMID: 18799732

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  • 亚细胞定位：
    Nucleus.
  • 组织特异性：
    Expressed in roots, leaf vasculature, shoot apical meristem (SAM) and developing anthers.
  • 数据库链接：

    KEGG: ath:AT3G62800

    STRING: 3702.AT3G62800.1

    UniGene: At.34049