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  4. Recombinant Arabidopsis thaliana FHA domain-containing protein DDL (DDL)

Recombinant Arabidopsis thaliana FHA domain-containing protein DDL (DDL)

  • 中文名称：
    Recombinant Arabidopsis thaliana FHA domain-containing protein DDL(DDL),Yeast
  • 货号：
    CSB-YP823826DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Arabidopsis thaliana FHA domain-containing protein DDL(DDL),Yeast
  • 货号：
    CSB-EP823826DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Arabidopsis thaliana FHA domain-containing protein DDL(DDL),Yeast
  • 货号：
    CSB-EP823826DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Arabidopsis thaliana FHA domain-containing protein DDL(DDL),Yeast
  • 货号：
    CSB-BP823826DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Arabidopsis thaliana FHA domain-containing protein DDL(DDL),Yeast
  • 货号：
    CSB-MP823826DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    DDL
  • Uniprot No.：
  • 别名：
    DDL; At3g20550; K10D20.9; FHA domain-containing protein DDL; Protein DAWDLE
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-314
  • 氨基酸序列
    MAPSSRSPSP RTKRLRRARG EKEIGRSRER EDDGREREKR NSRERDRDIG RDRDRERKGE GERDREVGDK RRRSGREDTE KRRRTRTDDE RYSRGRHERS TSPSDRSHRS SRRSPERAIA SRHDEGSNAR GGSEEPNVEE DSVARMRAVE EALAAKKKEE PSFELSGKLA EETNRYRGIT LLFNEPPEAR KPSERWRLYV FKDGEPLNEP LCLHRQSCYL FGRERRIADI PTDHPSCSKQ HAVIQYREME KEKPDGMMGK QVKPYIMDLG STNKTYINES PIEPQRYYEL FEKDTIKFGN SSREYVLLHE NSAE
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Involved in the microRNA (miRNA) and short interfering RNA (siRNA) biogenesis. May facilitate DCL1 to access or recognize primary miRNAs. Binds RNA non-specifically.
  • 基因功能参考文献：
    1. identified DDL as a physiological target of protein PARylation in plant immunity, which is uncoupled from its function in plant development PMID: 27797852
    2. the recognition of the target residue by the canonical binding cleft of the DDL FHA domain as the key molecular event to instate FHA domain-mediated protein-protein interaction in plant miRNA processing PMID: 23313986
    3. DDL is expressed in the root and shoot meristems and the reduced size of the root apical meristem in ddl plants suggests a role early in organ development. PMID: 16679419
    4. propose that DDL participates in miRNA biogenesis by facilitating DCL1 to access or recognize pri-miRNAs PMID: 18632581
  • 亚细胞定位：
    Nucleus.
  • 组织特异性：
    Expressed in roots, lateral roots, vascular strands of roots and leaves, vegetative meristems, pollen and developing seeds.
  • 数据库链接：

    KEGG: ath:AT3G20550

    STRING: 3702.AT3G20550.1

    UniGene: At.27430