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  4. Recombinant Arabidopsis thaliana Inositol-pentakisphosphate 2-kinase (IPK1)

Recombinant Arabidopsis thaliana Inositol-pentakisphosphate 2-kinase (IPK1)

  • 中文名称：
    拟南芥IPK1重组蛋白
  • 货号：
    CSB-YP842418DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    拟南芥IPK1重组蛋白
  • 货号：
    CSB-EP842418DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    拟南芥IPK1重组蛋白
  • 货号：
    CSB-EP842418DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    拟南芥IPK1重组蛋白
  • 货号：
    CSB-BP842418DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    拟南芥IPK1重组蛋白
  • 货号：
    CSB-MP842418DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    IPK1
  • Uniprot No.：
  • 别名：
    IPK1; At5g42810; MJB21.19; Inositol-pentakisphosphate 2-kinase; EC 2.7.1.158; Inositol-1,3,4,5,6-pentakisphosphate 2-kinase; Ins(1,3,4,5,6)P5 2-kinase; AtIPK1; InsP5 2-kinase
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-451
  • 氨基酸序列
    MEMILEEKDA SDWIYRGEGG ANLVLAYAGS SPLFVGKVIR IQKARRNDKA IKNANGVVSV LTSDEQHLWR ENNELISSPN KEVLEQRYVK NVIIPLLGPK HVDAGVRVSV SKEFLECVDK KVTKQRPLWR VNAANVDTSH DSALILNDHS LFSQGISSGG DCISVEIKPK CGFLPTSRFI GKENMLKTSV SRFKMHQLLK LEYNEISEES EYDPLDLFSG SKESVLEAIK ALYSTPQNNF RVFLNGSLIL GGSGESTGRT SPEIGYAFED ALKGFIQSED GHRTECFLQL VSDAVYGSGV LDRLLEIQKL DKLDIEGAIH SYYDLINQPC PICKEGKPLE AELSLHALPL DESLKIVKEY LIAATAKDCS IMISFQSRNA WDSEPSGDYV SLKPTNQTFD YKVHFIDLSL KPLKRMESYY KLDKKIISFY NRKQKAENTA EQIGNSKPSH S
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Phosphorylates Ins(1,3,4,5,6)P5 at position 2 to form Ins(1,2,3,4,5,6)P6 (InsP6 or phytate). Phytate is a regulator of intracellular signaling, a highly abundant animal antinutrient, and a phosphate store in plant seeds. Also phosphorylates Ins(1,3,4,6)P4 and Ins(1,4,5,6)P4 to produce Ins(1,2,3,4,6)P5 and Ins(1,2,4,5,6)P5.
  • 基因功能参考文献：
    1. a particular mechanism of Arsenic(V) toxicity existed in atipk1-1 mutant. PMID: 26264234
    2. Expression of the Gle1 variants with enhanced Myo-inositol-1,2,3,4,5,6-hexakisphosphate (InsP(6)) sensitivity rescues the mRNA export defect of the ipk1 (inositol 1,3,4,5,6-pentakisphosphate 2-kinase) InsP(6)-deficient mutant. PMID: 25670768
    3. IPK is indeed a member of the plant terpenoid metabolic network. PMID: 26216978
    4. AtIPK1 has important roles in growth and phosphate (Pi) homeostasis. PMID: 25155524
    5. Conformational changes in inositol 1,3,4,5,6-pentakisphosphate 2-kinase upon substrate binding: role of N-terminal lobe and enantiomeric substrate preference. PMID: 22745128
    6. Report that limited proteolysis has revealed the presence of multiple conformational states in the IPK1 catalytic cycle and propose a model for recognition of IP substrate by IPK1. PMID: 22362712
    7. The isolation and characterization of ATIPK1 is reported. PMID: 16223361

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  • 蛋白家族：
    IPK1 type 2 family
  • 组织特异性：
    Strongly expressed in leaves and cauline leaves. Weakly expressed in siliques and flowers. In flower, it is expressed in the major organs of developing flower buds. Strongly expressed in sepals, petals, in the male and female organs of immature and mature
  • 数据库链接：

    KEGG: ath:AT5G42810

    STRING: 3702.AT5G42810.1

    UniGene: At.8456