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  4. Recombinant Arabidopsis thaliana Mannan endo-1,4-beta-mannosidase 7 (MAN7)

Recombinant Arabidopsis thaliana Mannan endo-1,4-beta-mannosidase 7 (MAN7)

  • 中文名称：
    拟南芥MAN7重组蛋白
  • 货号：
    CSB-YP861819DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    拟南芥MAN7重组蛋白
  • 货号：
    CSB-EP861819DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    拟南芥MAN7重组蛋白
  • 货号：
    CSB-EP861819DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    拟南芥MAN7重组蛋白
  • 货号：
    CSB-BP861819DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    拟南芥MAN7重组蛋白
  • 货号：
    CSB-MP861819DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    MAN7
  • Uniprot No.：
  • 别名：
    MAN7; At5g66460; K1F13.12Mannan endo-1,4-beta-mannosidase 7; EC 3.2.1.78; Beta-mannanase 7; Endo-beta-1,4-mannanase 7; AtMAN7
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    26-431
  • 氨基酸序列
    LPSGG FVRTKGVQFS LNGYPYYANG FNAYWLMYVA SDPSQRSKIS TAFQDASRHG LTVARTWAFS DGGYRALQYS PGSYNEDMFQ GLDFALAEAR RHGIKIILSF ANNYESFGGR KQYVDWARSR GRPVSSEDDF FTDSLVKDFY KNHIKAVLNR FNTFTKVHYK DDPTIMAWEL MNEPRCPSDP SGRAIQAWIT EMAAHVKSLD RNHLLEAGLE GFYGQSSPQS KTLNPPGQFG TDFIANNRIP GIDFVTVHSY PDEWFPDSSE QSQMDFLNKW LDAHIQDAQN VLHKPIILAE FGKSMKKPGY TPAQRDIVFN TVYSKIYGSA KRGGAAAGGL FWQLLVNGID NFQDGYGIIL SQSSSTVNVI SQQSRKLTLI RKIFARMINV EKWKRARGQG QVGKRGHKIN N
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Required for both, loosening of the micropylar endosperm, and rupture of the seed coat in germinating seeds. May participate in the hydrolysis of the mannans in the cell wall of germinating seeds.
  • 基因功能参考文献：
    1. Data indicate that the expression of AtMAN7 gene (At5g66460) gene drastically reduced in the knock-out lines of AtbZIP44 (At1g75390). PMID: 23461773
    2. AtMAN7, AtMAN5 and specially AtMAN6 are important for the germination of A. thaliana seeds by facilitating the hydrolysis of the mannan-rich endosperm cell walls. [AtMAN7] PMID: 20878180
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Glycosyl hydrolase 5 (cellulase A) family
  • 组织特异性：
    Expressed in stems, flowers, siliques and seeds. Expressed in root vasculature, leaf hydathodes, anther filaments, stigma, sepal vasculature, at the base and apical parts of siliques, and replum. Expressed in the micropylar endosperm and radicle tip in ea
  • 数据库链接：

    KEGG: ath:AT5G66460

    STRING: 3702.AT5G66460.1

    UniGene: At.28865