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  4. Recombinant Arabidopsis thaliana Probable WRKY transcription factor 53 (WRKY53)

Recombinant Arabidopsis thaliana Probable WRKY transcription factor 53 (WRKY53)

  • 中文名称：
    拟南芥WRKY53重组蛋白
  • 货号：
    CSB-YP890447DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    拟南芥WRKY53重组蛋白
  • 货号：
    CSB-EP890447DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    拟南芥WRKY53重组蛋白
  • 货号：
    CSB-EP890447DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    拟南芥WRKY53重组蛋白
  • 货号：
    CSB-BP890447DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    拟南芥WRKY53重组蛋白
  • 货号：
    CSB-MP890447DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    WRKY53
  • Uniprot No.：
  • 别名：
    WRKY53; At4g23810; F9D16.280Probable WRKY transcription factor 53; WRKY DNA-binding protein 53
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-324
  • 氨基酸序列
    MEGRDMLSWE QKTLLSELIN GFDAAKKLQA RLREAPSPSS SFSSPATAVA ETNEILVKQI VSSYERSLLL LNWSSSPSVQ LIPTPVTVVP VANPGSVPES PASINGSPRS EEFADGGGSS ESHHRQDYIF NSKKRKMLPK WSEKVRISPE RGLEGPQDDV FSWRKYGQKD ILGAKFPRSY YRCTHRSTQN CWATKQVQRS DGDATVFEVT YRGTHTCSQA ITRTPPLASP EKRQDTRVKP AITQKPKDIL ESLKSNLTVR TDGLDDGKDV FSFPDTPPFY NYGTINGEFG HVESSPIFDV VDWFNPTVEI DTTFPAFLHE SIYY
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcription factor. Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element. May regulate the early events of leaf senescence. Negatively regulates the expression of ESR/ESP. Together with WRKY46 and WRKY70, promotes resistance to P.syringae, probably by enhancing salicylic acid (SA)- dependent genes. Contributes to the suppression of jasmonic acid (MeJA)-induced expression of PDF1.2.
  • 基因功能参考文献：
    1. The findings provide strong support for the direct regulatory roles of TGA3 and WRKY53 in the salicylic acid (SA) and NPR1-dependent activation of a Caulimoviral promoter (CmYLCV). PMID: 28913593
    2. activated expression inhibits stomatal closure by reducing H2O2 content and facilitates stomatal opening by promoting starch degradation PMID: 25861729
    3. WRKY53 is a master regulator of age-induced leaf senescence. PMID: 25395454
    4. ATL31 plays a role in senescence together with the WRKY53 transcription factor. PMID: 24399238
    5. WHY1 was an upstream regulator of WRKY53 during leaf senescence. PMID: 23922267
    6. These results suggest that WRKY53 and ESR mediate negative crosstalk between pathogen resistance and senescence, which is most likely governed by the jasmonic acid and salicylic acid equilibrium. PMID: 17369373
    7. WRKY53 and HSPRO2 appear to function downstream of salicylic acid and to be negatively regulated by signaling through jasmonic acid and ethylene. PMID: 17977154
    8. Heterochromatic silencing by histone methylation of WRKY53 controls leaf senescence. PMID: 19143996

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    WRKY group III family
  • 数据库链接：

    KEGG: ath:AT4G23810

    STRING: 3702.AT4G23810.1

    UniGene: At.2572