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  4. Recombinant Arabidopsis thaliana Probable WRKY transcription factor 70 (WRKY70)

Recombinant Arabidopsis thaliana Probable WRKY transcription factor 70 (WRKY70)

  • 中文名称：
    拟南芥WRKY70重组蛋白
  • 货号：
    CSB-YP862927DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    拟南芥WRKY70重组蛋白
  • 货号：
    CSB-EP862927DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    拟南芥WRKY70重组蛋白
  • 货号：
    CSB-EP862927DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    拟南芥WRKY70重组蛋白
  • 货号：
    CSB-BP862927DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    拟南芥WRKY70重组蛋白
  • 货号：
    CSB-MP862927DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    WRKY70
  • Uniprot No.：
  • 别名：
    WRKY70; At3g56400; T5P19.50Probable WRKY transcription factor 70; WRKY DNA-binding protein 70
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-294
  • 氨基酸序列
    MDTNKAKKLK VMNQLVEGHD LTTQLQQLLS QPGSGLEDLV AKILVCFNNT ISVLDTFEPI SSSSSLAAVE GSQNASCDND GKFEDSGDSR KRLGPVKGKR GCYKRKKRSE TCTIESTILE DAFSWRKYGQ KEILNAKFPR SYFRCTHKYT QGCKATKQVQ KVELEPKMFS ITYIGNHTCN TNAETPKSKT CDHHDEIFMD SEDHKSPSLS TSMKEEDNPH RHHGSSTEND LSLVWPEMVF EEDYHHQASY VNGKTSTSID VLGSQDLMVF GGGGDFEFSE NEHFSIFSSC SNLS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcription factor involved in senescence, biotic and abiotic stress responses by modulating various phytohormones signaling pathways. Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element. Binds to the 5'-[CT]GACTTTT-3' motif in promoters of target genes to induce their expression. Plays an important but not indispensable role in jasmonate and salicylic acid signaling. Regulates positively the salicylic acid (SA)-mediated signal pathway, but negatively the jasmonic acid (JA)-mediated signal pathway, thus determining the balance between these mutually antagonistic pathways. Together with WRKY46, WRKY53 and WRKY54, prevents defense response to the necrotrophic pathogens P.carotovorum and B.cinerea, but promotes defense responses (including SA-induced pathogenesis-related (PR) genes expression) against biotrophic/hemibiotrophic SA-monitored pathogens (e.g. P.syringae, E.carotovora subsp. carotovora SCC3193 and E.cichoracearum), probably by regulating negatively the JA/ET and positively the SA signaling pathways. Contributes to the suppression of jasmonic acid (MeJA)-induced expression of JA-responsive genes (e.g. PDF1.2). Promotes susceptibility to JA-monitored pathogens (e.g. A.brassicicola), probably by facilitating SA-controlled suppression of JA-mediated defense. Represses the biosynthesis of the phytoalexin camalexin and indol-3-ylmethyl glucosinolate (IGS). Represses both SA and JA/ethylene (ET) mediated defense marker genes expression. Negative regulator of SA biosynthesis. Negative regulator of EDS1-dependent defense against E.amylovora. Required for RPP4-mediated disease resistance and basal defense against H.parasitica, probably via late up-regulation (LURP) of resistance genes (e.g. CML10/CaBP22 and LURP1). Probably involved in defense responses toward insects (e.g. P.xylostella and B.brassicae). Together with WRKY54, negative regulator of developmental senescence, probably via the regulation of several senescence-associated markers genes. Together with WRKY46 and WRKY54, promotes brassinosteroid (BR)-regulated plant growth but prevent drought response by modulating gene expression. In collaboration with WRKY54, prevents stomatal closure and, consequently, osmotic stress tolerance. Regulates rhizobacterium B.cereus AR156-induced systemic resistance (ISR) to P.syringae pv. tomato DC3000.
  • 基因功能参考文献：
    1. Bacillus cereus AR156 treatment significantly stimulated the transcription of WRKY70, but suppressed that of WRKY11 in Arabidopsis leaves. They were shown to be required for AR156 enhancing the activation of cellular defence responses. PMID: 26433201
    2. A lower expression level of WRKY70 leads to significantly higher MYC2 expression through salicylic acid (SA)-jasmonic acid (JA) cross-talk. PMID: 25339349
    3. AtMYB44 regulates defense responses by transcriptional activation of downstream gene, WRKY70. PMID: 23603962
    4. Data indicate that the nucleotides CGAC are essential for WRKY70-activated gene expression. PMID: 24104863
    5. AtMYB44 modulates antagonistic interaction by activating SA-mediated defenses and repressing JA-mediated defenses through direct control of WRKY70 PMID: 23067202
    6. WRKY70 is required for snc2-1D-mediated resistance. PMID: 20841424
    7. WRKY70 has a pivotal role in determining the balance between SA-dependent and JA-dependent defense pathways. PMID: 16623907
    8. To expand understanding on how transcriptional control coordinates leaf senescence, AtWRKY70, a gene encoding a WRKY transcription factor that functions as a negative regulator of developmental senescence was characterized. PMID: 17310369
    9. The inactivation of the WRKY70 gene in wrky70-1 mutant does not alter the responses of both jasmonic acid (JA) and salicylic acid (SA), and that wrky70 mutation is unable to restore the coi1 mutant in JA responses. PMID: 18713432

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    WRKY group III family
  • 组织特异性：
    Expressed in leaves and flowers.
  • 数据库链接：

    KEGG: ath:AT3G56400

    STRING: 3702.AT3G56400.1

    UniGene: At.5242