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  4. Recombinant Bacillus subtilis Flagellar motor switch phosphatase FliY (fliY)

Recombinant Bacillus subtilis Flagellar motor switch phosphatase FliY (fliY)

  • 中文名称：
    枯草芽孢杆菌fliY重组蛋白
  • 货号：
    CSB-YP326115BRJ
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    枯草芽孢杆菌fliY重组蛋白
  • 货号：
    CSB-EP326115BRJ
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    枯草芽孢杆菌fliY重组蛋白
  • 货号：
    CSB-EP326115BRJ-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    枯草芽孢杆菌fliY重组蛋白
  • 货号：
    CSB-BP326115BRJ
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    枯草芽孢杆菌fliY重组蛋白
  • 货号：
    CSB-MP326115BRJ
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    fliY
  • Uniprot No.：
  • 别名：
    fliY; cheD; BSU16320Flagellar motor switch phosphatase FliY; EC 3.-.-.-; CheY-P phosphatase FliY; Flagellar motor switch protein FliY
  • 种属：
    Bacillus subtilis (strain 168)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-378
  • 氨基酸序列
    MENNRLSQDE IDALLNGTGS TLDEPEIPEV DDLSEMERDA IGEIGNISFG SSATALSTLL NQKVDITTPS VTVIPRSKIS DAFPEPYVAI EVNYTEGFSG SNLLVVEQSD AAIIADLMIG GDGKGADPSL GEIHLSAVQE AMNQMMGSAA TSMSTVFSKK IDISPPRVEL LDVTEEKGTD RIPDDEMLVK VSFNLKVGEL IDSSIMQLYP LTFAKDLISS LMNSESAEEE ETVQPEVTYE QPKEPVTPEP RIEPKQQQQP PKRQGTAKKA APVQVSPVEF SAFDPNEAVQ APIHNLDMLL DIPLSITVEL GRTKRSVKEI LELSAGSIIE LDKLAGEPVD ILVNQRIVAK GEVVVIEENF GVRVTDILSQ AERINNLK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Component of the flagellar switch. Binds CheY-P and increases its hydrolysis rate in vitro. May function constitutively to remove CheY-P around the flagellar switch to maintain an optimal level of CheY-P whereas CheC may function after addition of an attractant to cope with increased levels of CheY-P.
  • 亚细胞定位：
    Cell membrane; Peripheral membrane protein; Cytoplasmic side.
  • 蛋白家族：
    FliN/MopA/SpaO family
  • 数据库链接：

    KEGG: bsu:BSU16320

    STRING: 224308.Bsubs1_010100009006