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Recombinant Clostridium perfringens Perfringolysin O (pfo)

In Stock
  • 货号：
    CSB-EP314820CMB
  • 规格：
    ￥2328
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
    • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP314820CMB could indicate that this peptide derived from E.coli-expressed Clostridium perfringens (strain 13 / Type A) pfo.
    • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP314820CMB could indicate that this peptide derived from E.coli-expressed Clostridium perfringens (strain 13 / Type A) pfo.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 别名：
    pfo; pfoA; pfoR; CPE0163; Perfringolysin O; Theta-toxin; Thiol-activated cytolysin
  • 种属：
    Clostridium perfringens (strain 13 / Type A)
  • 蛋白长度：
    Full Length of Mature Protein
  • 来源：
    E.coli
  • 分子量：
    68.7kDa
  • 表达区域：
    29-500aa
  • 氨基酸序列
    KDITDKNQSIDSGISSLSYNRNEVLASNGDKIESFVPKEGKKTGNKFIVVERQKRSLTTSPVDISIIDSVNDRTYPGALQLADKAFVENRPTILMVKRKPININIDLPGLKGENSIKVDDPTYGKVSGAIDELVSKWNEKYSSTHTLPARTQYSESMVYSKSQISSALNVNAKVLENSLGVDFNAVANNEKKVMILAYKQIFYTVSADLPKNPSDLFDDSVTFNDLKQKGVSNEAPPLMVSNVAYGRTIYVKLETTSSSKDVQAAFKALIKNTDIKNSQQYKDIYENSSFTAVVLGGDAQEHNKVVTKDFDEIRKVIKDNATFSTKNPAYPISYTSVFLKDNSVAAVHNKTDYIETTSTEYSKGKINLDHSGAYVAQFEVAWDEVSYDKEGNEVLTHKTWDGNYQDKTAHYSTVIPLEANARNIRIKARECTGLAWEWWRDVISEYDVPLTNNINVSIWGTTLYPGSSITYN
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-SUMO-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：
        Recombinant Clostridium perfringens Perfringolysin O (pfo)是一种经重组制备的产气荚膜梭菌（Clostridium perfringens）溶原性毒素O蛋白。Perfringolysin O是产气荚膜梭菌的一个主要毒素，它能够破坏目标细胞的细胞膜，导致细胞死亡。Perfringolysin O的研究对于了解产气荚膜梭菌感染机制和溶原性毒素的生物学特性具有重要意义。
        j9九游会登录入口首页生物的重组pfo蛋白，由大肠杆菌表达系统表达，具有良好的稳定性和纯度，可以更好帮助您研究其在细胞毒性和感染过程中的作用机制，以及与宿主细胞相互作用的方式；可以被广泛应用于生物医学研究中，如细胞毒性实验、毒力机制研究和免疫学研究等。
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    A cholesterol-dependent toxin that causes cytolysis by forming pores in cholesterol-containing host membranes. After binding to target membranes, the protein assembles into a pre-pore complex. A major conformational change leads to insertion in the host membrane and formation of an oligomeric pore complex. Cholesterol is required for binding to host cell membranes, membrane insertion and pore formation; cholesterol binding is mediated by a Thr-Leu pair in the C-terminus. Can be reversibly inactivated by oxidation.
  • 亚细胞定位：
    Secreted. Host cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Thiol-activated cytolysin family
  • 数据库链接：

    KEGG: cpe:CPE0163