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  4. Recombinant Danio rerio Protein lin-28 homolog A (lin28a)

Recombinant Danio rerio Protein lin-28 homolog A (lin28a)

  • 中文名称：
    斑马鱼lin28a重组蛋白
  • 货号：
    CSB-YP768841DIL
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    斑马鱼lin28a重组蛋白
  • 货号：
    CSB-EP768841DIL
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    斑马鱼lin28a重组蛋白
  • 货号：
    CSB-EP768841DIL-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    斑马鱼lin28a重组蛋白
  • 货号：
    CSB-BP768841DIL
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    斑马鱼lin28a重组蛋白
  • 货号：
    CSB-MP768841DIL
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    lin28a
  • Uniprot No.：
  • 别名：
    lin28a; lin28; si:ch211-232d9.4; zgc:55584; Protein lin-28 homolog A; Lin-28A
  • 种属：
    Danio rerio (Zebrafish) (Brachydanio rerio)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-202
  • 氨基酸序列
    MPPANPHLNH TGGCTKTEEE EAASSEEDSG SFHGSGVCKW FNVRMGFGFL SMTHREGICL DSPVDVFVHQ SKLHMEGFRS LKEGEAVEFT FKRSSKGLES LQVTGPGGAP CVGSEKKPKG TQKRRSKGDR CFNCGGPNHH AKECQLPPQP KKCHFCQSIS HMVANCPIKA QQLSPGSQGK STTSTGEEED MSHTPLLPES TD
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    RNA-binding protein that inhibits processing of pre-let-7 miRNAs and regulates translation of mRNAs that control developmental timing, pluripotency and metabolism. Seems to recognize a common structural G-quartet (G4) feature in its miRNA and mRNA targets. 'Translational enhancer' that drives specific mRNAs to polysomes and increases the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in mRNA stabilization. Suppressor of microRNA (miRNA) biogenesis, including that of let-7. Binds specific target miRNA precursors (pre-miRNAs), recognizing an 5'-GGAG-3' motif found in their terminal loop, and recruits uridylyltransferase. This results in the terminal uridylation of target pre-miRNAs. Uridylated pre-miRNAs fail to be processed by Dicer and undergo degradation. Localized to the periendoplasmic reticulum area, binds to a large number of spliced mRNAs and inhibits the translation of mRNAs destined for the ER, reducing the synthesis of transmembrane proteins, ER or Golgi lumen proteins, and secretory proteins. Binds to and enhances the translation of mRNAs for several metabolic enzymes, increasing glycolysis and oxidative phosphorylation. Which, with the let-7 repression may enhance tissue repair in adult tissue.
  • 基因功能参考文献：
    1. The knockdown of Lin-28a or Lin-28b function by morpholino microinjection into embryos resulted in severe cell proliferation defects during early morphogenesis. PMID: 24516585
    2. The Lin-28 is induced in Muller glia within 6 h following retinal injury and is necessary for Muller glia dedifferentiation. PMID: 20935637
  • 亚细胞定位：
    Cytoplasm. Rough endoplasmic reticulum. Cytoplasm, P-body. Cytoplasm, Stress granule. Nucleus, nucleolus.
  • 蛋白家族：
    Lin-28 family
  • 数据库链接：

    KEGG: dre:394066

    STRING: 7955.ENSDARP00000089741

    UniGene: Dr.24217