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  4. Recombinant Drosophila melanogaster Homeobox protein extradenticle (exd)

Recombinant Drosophila melanogaster Homeobox protein extradenticle (exd)

  • 中文名称：
    黑腹果蝇exd重组蛋白
  • 货号：
    CSB-YP328114DLU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    黑腹果蝇exd重组蛋白
  • 货号：
    CSB-EP328114DLU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    黑腹果蝇exd重组蛋白
  • 货号：
    CSB-EP328114DLU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    黑腹果蝇exd重组蛋白
  • 货号：
    CSB-BP328114DLU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    黑腹果蝇exd重组蛋白
  • 货号：
    CSB-MP328114DLU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    exd
  • Uniprot No.：
  • 别名：
    exd; CG8933Homeobox protein extradenticle; Dpbx
  • 种属：
    Drosophila melanogaster (Fruit fly)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-376
  • 氨基酸序列
    MEDPNRMLAH TGGMMAPQGY GLSGQDDGQN AGSENEVRKQ KDIGEILQQI MSISEQSLDE AQARKHTLNC HRMKPALFSV LCEIKEKTVL SIRNTQEEEP PDPQLMRLDN MLIAEGVAGP EKGGGGAAAA SAAAASQGGS LSIDGADNAI EHSDYRAKLA QIRQIYHQEL EKYEQACNEF TTHVMNLLRE QSRTRPITPK EIERMVQIIH KKFSSIQMQL KQSTCEAVMI LRSRFLDARR KRRNFSKQAS EILNEYFYSH LSNPYPSEEA KEELARKCGI TVSQVSNWFG NKRIRYKKNI GKAQEEANLY AAKKAAGASP YSMAGPPSGT TTPMMSPAPP QDSMGYPMGS GGYDQQQPYD NSMGGYDPNL HQDLSP
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcription factor which acts with the selector homeodomain proteins altering the regulation of downstream target genes such as wingless (wg), teashirt (tsh) and decapentaplegic (dpp), thus affecting segmental identity. Delimits the eye field and prevent inappropriate eye development. Required for proper localization of chordotonal organs within the peripheral nervous system.
  • 基因功能参考文献：
    1. Hth functions together with its co-factor Extradenticle to repress the R8-specific factor Senseless in dorsal rim area R8 cells, allowing expression of an ultraviolet-sensitive R7 Rhodopsin. PMID: 24496628
    2. cooperative binding sites for En, with the homeodomain-containing Hox cofactors Extradenticle (Exd) and Homothorax (Hth) was localized, within two CRMs that drive similar expression patterns PMID: 22537495
    3. In the flight muscles, exd and hth are genetically upstream of another muscle identity gene, salm, and are direct transcriptional regulators of the signature flight muscle structural gene, Actin88F. PMID: 22975331
    4. Using computational platform, SELEX-seq, study shows that all eight Drosophila Hox proteins obtain novel recognition properties when they bind DNA with the dimeric cofactor Extradenticle-Homothorax. PMID: 22153072
    5. Homothorax and extradenticle control dendritic and axonal targeting of olfactory projection neurons in the Drosophila brain. PMID: 21801717
    6. Selection of distinct Hox-Extradenticle interaction modes fine-tunes Hox protein activity. PMID: 21262810
    7. Hox repression of a target gene: extradenticle-independent, additive action through multiple monomer binding sites. PMID: 12070087
    8. in thoracic segments, Exd is required for repression (as well as activation) by En. This is consistent with in vitro results showing that Exd is involved in recognition of positive and negative targets. PMID: 12506003
    9. we show that the functions of both of the Hox cofactors Extradenticle and Homothorax play essential roles in repression by Engrailed. PMID: 12506004
    10. Labial, Ultrabithorax, Antennapedia, Extradenticle and Homothorax are regulated in a signal transduction pathway in developing Drosophila brain PMID: 15172684
    11. homothorax/extradenticle is repressed in the body regions where eyg is active PMID: 15634705
    12. distinct Hox/Exd/Hth complexes select different binding sites, and a given complex recognizes a consensus DNA sequence PMID: 15753213
    13. Extradenticle and Homothorax selector proteins control branchless/FGF expression in mesodermal bridge-cells PMID: 16007069
    14. nuclear EXD is more extensively phosphorylated than the cytoplasmic form, suggesting that EXD is a target of signal transduction by protein kinases PMID: 16403493
    15. Exd contains an NLS mask that contributes to its cytoplasmic localization. PMID: 17277370
    16. This finding highlights that the Hox protein Ubx has multiple ways to interact with the Exd cofactor and suggests that flexibility in Hox-PBC contacts contributes to specify and diversify Hox protein function. PMID: 17942685

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  • 亚细胞定位：
    Nucleus. Note=Nuclear translocation requires interaction with hth.
  • 蛋白家族：
    TALE/PBX homeobox family
  • 组织特异性：
    Prior to full germband retraction it is ubiquitously present, after germband retraction, mostly present in the anterior portion of the ventral nerve cord.
  • 数据库链接：

    KEGG: dme:Dmel_CG8933

    STRING: 7227.FBpp0073940

    UniGene: Dm.174