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  4. Recombinant Drosophila melanogaster Protein scalloped (sd)

Recombinant Drosophila melanogaster Protein scalloped (sd)

  • 中文名称：
    黑腹果蝇sd重组蛋白
  • 货号：
    CSB-YP333498DLU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    黑腹果蝇sd重组蛋白
  • 货号：
    CSB-EP333498DLU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    黑腹果蝇sd重组蛋白
  • 货号：
    CSB-EP333498DLU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    黑腹果蝇sd重组蛋白
  • 货号：
    CSB-BP333498DLU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    黑腹果蝇sd重组蛋白
  • 货号：
    CSB-MP333498DLU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    sd
  • Uniprot No.：
  • 别名：
    sd; CG8544; Protein scalloped
  • 种属：
    Drosophila melanogaster (Fruit fly)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-440
  • 氨基酸序列
    MKNITSSSTC STGLLQLQNN LSCSELEVAE KTEQQAVGPG TIPSPWTPVN AGPPGALGSA DTNGSMVDSK NLDVGDMSDD EKDLSSADAE GVWSPDIEQS FQEALSIYPP CGRRKIILSD EGKMYGRNEL IARYIKLRTG KTRTRKQVSS HIQVLARRKL REIQAKIKVQ FWQPGLQPST SQDFYDYSIK PFPQPPYPAG KTSTAVSGDE TGIPPSQLPW EGRAIATHKF RLLEFTAFME IQRDEIYHRH LFVQLGGKPS FSDPLLETVD IRQIFDKFPE KSGGLKDLYE KGPQNAFYLV KCWADLNTDL TTGSETGDFY GVTSQYESNE NVVLVCSTIV CSFGKQVVEK VESEYSRLEN NRYVYRIQRS PMCEYMINFI QKLKNLPERY MMNSVLENFT ILQVMRARET QETLLCIAYV FEVAAQNSGT THHIYRLIKE
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcription factor which plays a key role in the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein Hippo (Hpo), in complex with its regulatory protein Salvador (Sav), phosphorylates and activates Warts (Wts) in complex with its regulatory protein Mats, which in turn phosphorylates and inactivates the Yorkie (Yki) oncoprotein. The Hippo/SWH signaling pathway inhibits the activity of the transcriptional complex formed by Scalloped (sd) and Yki and the target genes of this pathway include cyclin-E (cycE), diap1 and bantam. Sd promotes nuclear localization of Yki. Involved in the regulation of cell-specific gene expression during development, particularly in the differentiation of the nervous system. When in combination with vestigial (vg) it acts as a transcriptional activation complex that regulates gene expression in the wing. Binding to vg switches the DNA target selectivity of sd. Required autonomously for cell proliferation and viability within the wing blade. Required for proper sensory organ precursor (SOP) differentiation at the wing margin; required for correct expression of sens.
  • 基因功能参考文献：
    1. These findings suggest that spatiotemporal expression of transcription factor Sd induces differential growth regulation by Yki during wing disc development, highlighting coordination between Yki and CycE to control growth and maintain homeostasis. PMID: 28143945
    2. DNA-binding transcription factor Scalloped mediates both Yorkie overexpression and loss-of-function phenotypes yet is itself dispensable for normal eye development PMID: 29467233
    3. found that human E2F1 competes with YAP for TEAD1 binding, affecting YAP activity, indicating that this mode of cross-regulation is conserved PMID: 29207260
    4. Data show that Yorkie (Yki) and Scalloped (Sd) are required for epidermal wound closure in the larval epidermis. PMID: 28514643
    5. Vestigial protein phosphorylation of serine 215 occurs in the nucleus and requires the presence of Scalloped. PMID: 28322734
    6. Data indicate that the TEAD family transcription factor Scalloped (Scalloped) regulates PDGF/VEGFR receptor (Pvr) expression. PMID: 28322737
    7. Scalloped and Yorkie are required for cell cycle re-entry of quiescent cells after tissue damage. PMID: 26160905
    8. Yorkie and Scalloped are associated with specific cell-fate determination in the context of blood development. PMID: 25454586
    9. This study identifies a novel protein Sd-Binding-Protein, SdBP, which directly competes with Yki for binding to Sd through its TDU domains and inhibits the Sd-Yki transcriptional activity. PMID: 23999857
    10. Study describe the full breadth of SD expression during Drosophila embryogenesis, and identify a requirement for sd function in a subset of motor neurons. PMID: 23389965
    11. Yki controls normal tissue growth by relieving Sd-mediated default repression. PMID: 23725764
    12. These results support a critical role for Yki- and its partners Sd and Hth--in shaping the fate map of the eye epithelium. PMID: 21811580
    13. The identified nuclear localization signal is likely a classical bipartite signal. PMID: 21731746
    14. dE2F1 is needed for Yki/Sd-dependent full activation of these target genes, and a de2f1 mutation strongly blocks yki-induced proliferation in vivo. PMID: 21325133
    15. DNA target selectivity of Scalloped. PMID: 20725144
    16. the Vestigial/Scalloped transcription complex has a role during wing development in Drosophila melanogaster PMID: 12782275
    17. Drosophila sd is involved in wing development as well as neural development. PMID: 15126402
    18. Expression of the VGDeltaACT construct in the wing alters the cellular localization of VG and produces a mutant phenotype, indicating that the construct is able to antagonize the normal function of the SD/VG complex. PMID: 17110491
    19. Inactivation of Sd diminishes Hpo target gene expression and reduces organ size, whereas a constitutively active Sd promotes tissue overgrowth. PMID: 18258485
    20. identify the TEAD/TEF family protein Scalloped (Sd) as a DNA-binding transcription factor that partners with Yki to mediate the transcriptional output of the Hpo growth-regulatory pathway PMID: 18258486
    21. Interaction between YKI and SD increases SD transcriptional activity both ex vivo in Drosophila S2 cells and in vivo in Drosophila wing discs and promotes YKI nuclear localization. PMID: 18313299
    22. Data show that both Vestigial and Scalloped are required for muscle differentiation, as loss of either leads to a loss of a subset of cardiac or somatic muscle cells in developing embryos, and also that Sd, Vg, and Dmef2 can interact directly. PMID: 18987343
    23. at least one of the modifiers is linked to the vestigial region and demonstrate that the background effects modify the spatial distribution of known sd target genes in a genotype-dependent manner PMID: 19064709

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  • 亚细胞定位：
    Nucleus.
  • 组织特异性：
    Subset of neuroblasts in the central nervous system and in the peripheral sense organs of the embryo. Expressed in the developing wing primordia initially along the D/V wing boundary, and by the late third larval instar, maximal expression is seen in cell
  • 数据库链接：

    KEGG: dme:Dmel_CG8544

    STRING: 7227.FBpp0301994

    UniGene: Dm.6072