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  4. Recombinant Drosophila melanogaster Protein slit (sli)

Recombinant Drosophila melanogaster Protein slit (sli)

  • 中文名称：
    黑腹果蝇sli重组蛋白
  • 货号：
    CSB-YP338562DLU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    黑腹果蝇sli重组蛋白
  • 货号：
    CSB-EP338562DLU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    黑腹果蝇sli重组蛋白
  • 货号：
    CSB-EP338562DLU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    黑腹果蝇sli重组蛋白
  • 货号：
    CSB-BP338562DLU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    黑腹果蝇sli重组蛋白
  • 货号：
    CSB-MP338562DLU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    sli
  • Uniprot No.：
  • 别名：
    sli; CG43758; Protein slit; dSlit) [Cleaved into: Protein slit N-product; Protein slit C-product]
  • 种属：
    Drosophila melanogaster (Fruit fly)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    A short-range repellent, controlling axon crossing of the midline and a long-range chemorepellent, controlling mesoderm migration and patterning away from the midline. May interact with extracellular matrix molecules. Repulsive ligand for the guidance receptor roundabout (robo) and prevents inappropriate midline crossing by Robo-expressing axons.
  • 基因功能参考文献：
    1. Vascular cells produce Slit that activates Robo receptors in the posterior signalling centre of the larval haematopoietic organ, the lymph gland. PMID: 27193394
    2. loss of Atg16 or its binding partner Rab19 impairs Slit production, which normally inhibits enteroendocrine cell generation by activating Robo signaling in stem cells. PMID: 28982685
    3. repulsive Slit-Robo2-Ena signaling exerts an extrusive force downstream of JNK to eliminate scrib cells from epithelia by disrupting E-cadherin. PMID: 27997825
    4. These results suggest that Slit cleaveage, and the formation of Slit-N oligomers on the tendon cell surfaces direct muscle elongation, provide a stop signal for the approaching muscle. PMID: 26554435
    5. Slit-binding is necessary for its repulsive signaling output PMID: 26335920
    6. a Robo2-mediated mechanism, independent of signaling, is essential to limiting Slit distribution and which might be relevant to the regulation of Slit-mediated short-range signaling in additional systems PMID: 26400093
    7. Data show that Slit cleavage restricts its distribution to tendon cells, allowing it to function as a short-range repellent that directs muscle migration and patterning, and promotes their halt upon reaching the target site. PMID: 25813540
    8. these findings show that the polarized localization of Mp controls the direction, timing, and presumably the extent of Slit/Robo activity and signaling at the luminal membrane of the heart cardioblasts. PMID: 23825967
    9. Distinct functional domains of the Abelson tyrosine kinase control axon guidance responses to Netrin and Slit to regulate the assembly of neural circuits PMID: 23720041
    10. the Slit/Roundabout pathway, best known for its role in regulating axonal guidance, is essential for proper gonad formation PMID: 21377458
    11. during normal development, gradients of Slit protein repel axons away from compartment boundaries - in consequence, the bristles from each segment send their nerves to the CNS in separated sets PMID: 20667917
    12. At the CNS midline, Slit functions as a repellent for tracheal branches and this function is mediated primarily by Robo. Robo2 is necessary for the tracheal response to the attractive Slit signal and its function is antagonized by Robo. PMID: 12397103
    13. midline-secreted Slit activates Robo/Robo2 signalling to control the migration of the vmd1a sensory precursor cells PMID: 15147761
    14. schizo regulates Slit expression via endocytosis PMID: 15148300
    15. loss of a neutralizing effect on the Netrin-mediated attraction is responsible for the longitudinal axonal tracts entering the midline in slit mutants PMID: 15716500
    16. Slit and Robo proteins contribute to heart morphogenesis by guiding cell alignment and adhesion and/or by inhibiting cell mixing between the bilateral compartments of heart cell progenitors and ensuring proper polarity of the myocardial epithelium. PMID: 16360689
    17. slit is required for heart assembly PMID: 16516189
    18. Results describe the role of Slit and its transmembrane receptors Roundabout (Robo) and Roundabout2 (Robo2) during morphogenesis of the Drosophila heart tube, a process analogous to early heart formation in vertebrates. PMID: 16888037
    19. Slit is present outside of the midline along the longitudinal and commissural tracts. PMID: 17565966
    20. Data show that on binding of Slit to Robo, the adaptor protein Cables is recruited to Robo-associated Abl and forms a multimeric complex by binding directly to N-cadherin-associated beta-catenin. PMID: 17618275
    21. Slit and Robo control the development of dendrites in the embryonic Central Nervous System PMID: 17933790
    22. Our data show that Slit and Robo pathways function in lumen formation as a repulsive signal to antagonize E-Cad-mediated cell adhesion. PMID: 18663139
    23. Slit, through Robo, provides an extrinsic signal to coordinate the growth rate and the branching level of space-filling neurons, thus allowing them to appropriately cover their target field. PMID: 18817767
    24. Dlp, which lacks chondroitin sulfate (CS)modifications, participates in the transfer of Slit from its site of expression to the target cells, where CS-modified Sdc concentrates and presents the ligand. PMID: 19574454

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  • 亚细胞定位：
    Secreted.
  • 组织特异性：
    In embryos, highest expression occurs around the midline glia and low expression is observed around CNS axons lateral to the midline. Expression can be seen on the commissural axons traversing the glial cells but it is absent from the cell bodies of these
  • 数据库链接：

    KEGG: dme:Dmel_CG43758

    STRING: 7227.FBpp0303578

    UniGene: Dm.4729