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  4. Recombinant Drosophila melanogaster Protein split ends (spen)

Recombinant Drosophila melanogaster Protein split ends (spen)

  • 中文名称：
    黑腹果蝇spen重组蛋白
  • 货号：
    CSB-YP854742DLU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    黑腹果蝇spen重组蛋白
  • 货号：
    CSB-EP854742DLU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    黑腹果蝇spen重组蛋白
  • 货号：
    CSB-EP854742DLU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    黑腹果蝇spen重组蛋白
  • 货号：
    CSB-BP854742DLU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    黑腹果蝇spen重组蛋白
  • 货号：
    CSB-MP854742DLU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    spen
  • Uniprot No.：
  • 别名：
    spen; CG18497; Protein split ends
  • 种属：
    Drosophila melanogaster (Fruit fly)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Probable corepressor protein, which regulates different key pathways such as the EGF receptor and Wg pathways. Involved in neuronal cell fate, survival and axon guidance, cell cycle regulation and repression of head identity in the embryonic trunk. May act with the Hox gene Deformed and the EGF receptor signaling pathway. Positive regulator of the Wg pathway in larval tissues but not in embryonic tissues. May act as a transcriptional corepressor protein, which repress transcription via the recruitment of large complexes containing histone deacetylase proteins.
  • 基因功能参考文献：
    1. Spen is an important survival factor that ensures spatial control of the apoptotic wave that is necessary for the correct patterning and formation of the Drosophila eye. PMID: 25872184
    2. Split ends is a tissue/promoter specific regulator of Wingless signaling PMID: 12783785
    3. spen function is required for the correct formation and positioning of veins and mechanosensory bristles both on the anterior wing margin and on the notum, and for the maintenance of planar polarity PMID: 15596016
    4. Dosage-sensitive genetic interactions further support a model of functional antagonism between NITO and SPEN during Drosophila eye development. PMID: 16547102
    5. Plays a role in modulating output from the Notch and epidermal growth factor receptor pathways to ensure appropriate patterning during eye development. PMID: 17588724

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    RRM Spen family
  • 组织特异性：
    Ubiquitous. Expressed prior to cellularization in stage 3 embryos, and in blastoderm cells, including pole cells. Expressed throughout the rest of embryogenesis. Later, it is expressed at higher level in epidermal cells and CNS.
  • 数据库链接：

    KEGG: dme:Dmel_CG18497

    STRING: 7227.FBpp0077781

    UniGene: Dm.3450