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  4. Recombinant Enterobacteria phage P22 DNA-packaging protein gp3 (3)

Recombinant Enterobacteria phage P22 DNA-packaging protein gp3 (3)

  • 中文名称：
    Recombinant Enterobacteria phage P22 DNA-packaging protein gp3(3),Yeast
  • 货号：
    CSB-YP361406EDG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Enterobacteria phage P22 DNA-packaging protein gp3(3),Yeast
  • 货号：
    CSB-EP361406EDG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Enterobacteria phage P22 DNA-packaging protein gp3(3),Yeast
  • 货号：
    CSB-EP361406EDG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Enterobacteria phage P22 DNA-packaging protein gp3(3),Yeast
  • 货号：
    CSB-BP361406EDG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Enterobacteria phage P22 DNA-packaging protein gp3(3),Yeast
  • 货号：
    CSB-MP361406EDG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    3
  • Uniprot No.：
  • 别名：
    3Terminase; small subunit; DNA-packaging protein gp3
  • 种属：
    Enterobacteria phage P22 (Bacteriophage P22)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-162
  • 氨基酸序列
    AAPKGNRFW EARSSHGRNP KFESPEALWA ACCEYFEWVE ANPLWEMKAF SYQGEVIQEP IAKMRAMTIT GLTLFIDVTL ETWRTYRLRE DLSEVVTRAE QVIYDQKFSG AAADLLNANI IARDLGLKEQ SQVEDVTPDK GDRDKRRSRI KELFNRGTGR DS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    The terminase small subunit binds to the packaging initiation site and regulates the ATPase activity of the terminase large subunit. The terminase lies at a unique vertex of the procapsid and is composed of two subunits, a small terminase subunit involved in viral DNA recognition (packaging sequence), and a large terminase subunit possessing endonucleolytic and ATPase activities. Both terminase subunits heterooligomerize and are docked on the portal protein to form the packaging machine. The terminase large subunit exhibits endonuclease activity and cleaves the viral genome concatemer once the capsid is full (headful packaging). Once the capsid is packaged with the DNA, the terminase complex is substituted by neck proteins.
  • 基因功能参考文献：
    1. Results describe the purification and crystallization of P22 S-terminase, and show that it assembles into a concentration-independent nonamer of 168 kDa. PMID: 21206037
    2. Results suggest a plausible molecular mechanism for gp3 function in DNA recognition and translocation. PMID: 18775728
  • 蛋白家族：
    P22likevirus small terminase family
  • 数据库链接：

    KEGG: vg:2944227