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  4. Recombinant Escherichia coli ATP-dependent Clp protease adapter protein ClpS (clpS)

Recombinant Escherichia coli ATP-dependent Clp protease adapter protein ClpS (clpS)

  • 货号：
    CSB-YP359175ENV
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP359175ENV-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP359175ENV
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP359175ENV
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    clpS
  • Uniprot No.：
  • 别名：
    clpS; yljA; b0881; JW0865ATP-dependent Clp protease adapter protein ClpS
  • 种属：
    Escherichia coli (strain K12)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-106
  • 氨基酸序列
    MGKTNDWLDF DQLAEEKVRD ALKPPSMYKV ILVNDDYTPM EFVIDVLQKF FSYDVERATQ LMLAVHYQGK AICGVFTAEV AETKVAMVNK YARENEHPLL CTLEKA
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Involved in the modulation of the specificity of the ClpAP-mediated ATP-dependent protein degradation.
  • 基因功能参考文献：
    1. Between the halves of the clpA pseudo-dimer is a large flexible acidic loop that becomes better ordered upon binding of the small adaptor protein, ClpS PMID: 15037248
    2. the ClpAP-specific adaptor, ClpS, is essential for degradation of N-end rule substrates by ClpAP PMID: 16467841
    3. ClpAP recognizes N-end rule substrates directly, whereas ClpS modulates this degradation pathway. PMID: 17322400
    4. ClpS is an integral and essential component of the N-end rule pathway. PMID: 19317833
    5. The data suggest that ClpS has been optimized for the binding and delivery of N-degrons containing an N-terminal Phe or Leu. PMID: 19373253
    6. ClpS selectively binds N-terminal destabilizing residues and targets them for degradation by the ClpAP complex resulting in carefully regulated proteolysis PMID: 16467841

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  • 蛋白家族：
    ClpS family
  • 数据库链接：

    KEGG: ecj:JW0865

    STRING: 316385.ECDH10B_0951