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  4. Recombinant Escherichia coli Blue copper oxidase CueO (cueO)

Recombinant Escherichia coli Blue copper oxidase CueO (cueO)

  • 中文名称：
    Recombinant Escherichia coli Blue copper oxidase CueO(cueO),Yeast
  • 货号：
    CSB-YP339713ENV
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Escherichia coli Blue copper oxidase CueO(cueO),Yeast
  • 货号：
    CSB-EP339713ENV
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Escherichia coli Blue copper oxidase CueO(cueO),Yeast
  • 货号：
    CSB-EP339713ENV-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Escherichia coli Blue copper oxidase CueO(cueO),Yeast
  • 货号：
    CSB-BP339713ENV
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Escherichia coli Blue copper oxidase CueO(cueO),Yeast
  • 货号：
    CSB-MP339713ENV
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    cueO
  • Uniprot No.：
  • 别名：
    cueO; yacK; b0123; JW0119Blue copper oxidase CueO; Copper efflux oxidase
  • 种属：
    Escherichia coli (strain K12)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    29-516
  • 氨基酸序列
    AE RPTLPIPDLL TTDARNRIQL TIGAGQSTFG GKTATTWGYN GNLLGPAVKL QRGKAVTVDI YNQLTEETTL HWHGLEVPGE VDGGPQGIIP PGGKRSVTLN VDQPAATCWF HPHQHGKTGR QVAMGLAGLV VIEDDEILKL MLPKQWGIDD VPVIVQDKKF SADGQIDYQL DVMTAAVGWF GDTLLTNGAI YPQHAAPRGW LRLRLLNGCN ARSLNFATSD NRPLYVIASD GGLLPEPVKV SELPVLMGER FEVLVEVNDN KPFDLVTLPV SQMGMAIAPF DKPHPVMRIQ PIAISASGAL PDTLSSLPAL PSLEGLTVRK LQLSMDPMLD MMGMQMLMEK YGDQAMAGMD HSQMMGHMGH GNMNHMNHGG KFDFHHANKI NGQAFDMNKP MFAAAKGQYE RWVISGVGDM MLHPFHIHGT QFRILSENGK PPAAHRAGWK DTVKVEGNVS EVLVKFNHDA PKEHAYMAHC HLLEHEDTGM MLGFTV
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Probably involved in periplasmic detoxification of copper by oxidizing Cu(+) to Cu(2+) and thus preventing its uptake into the cytoplasm. Possesses phenoloxidase and ferroxidase activities and might be involved in the production of polyphenolic compounds and the prevention of oxidative damage in the periplasm.
  • 基因功能参考文献：
    1. Biochemical, spectroscopic and X-ray structural analysis of deuterated multicopper oxidase CueO has been presented. PMID: 27710945
    2. Exogenous acetate ion reaches the type II copper center in CueO through the water-excretion channel and potentially affects the enzymatic activity. PMID: 27380373
    3. the binding of copper to apo-CueO greatly stabilized the protein, indicating a transformation from an open or flexible domain arrangement with accessible copper sites to a closed structure with deeply buried copper ions. PMID: 27129241
    4. The results provides evidence that CueO possesses high Mn(II) oxidase activity and generates biogenic Mn oxide gamma-Mn3O4. PMID: 24699422
    5. At low levels of X-ray exposure, unambiguous electron density for an O atom is observed inside the trinuclear copper center (TNC); binding of oxygen to the TNC after its full reduction is observed in the case of the laccase. PMID: 24598746
    6. Laccase activities of CueO were maximally enhanced 140-fold by virtue of the synergistic effect of mild mutations at and at around the ligand groups to type I copper. PMID: 23337502
    7. These results exerted on the hydrogen bond network in CueO are discussed in comparison with proton transfers in cytochrome oxidase. PMID: 22564733
    8. The results revealed that the crude CueO catalyzed the oxidation of anthracene and benzo[a]pyrene in the same way as the fungal laccase from Trametes versicolor, but showed specific characteristics such as thermostability and copper dependence. PMID: 21120471
    9. has robust cuprous oxidase activity which is central to the mechanism by which CueO protects E coli against copper toxicity PMID: 15516598
    10. Copper toxicity towards a cueO mutant could be suppressed by addition of the superoxide quencher 1,2-dihydroxybenzene-3,5-disulfonic acid (tiron), suggesting that a primary cause of copper toxicity is the copper-catalyzed production of superoxide anions. PMID: 15708004
    11. Transcriptional profiling showed that expression of genes associated with motility was lowered in a cueO mutant while expression of genes associated with autoaggregation was elevated. PMID: 17635554
    12. Data indicate that the methionine-rich alpha-helices of CueO function as a barrier to the access of bulky organic substrates, which provides CueO with specificity as a cuprous oxidase. PMID: 17804014

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  • 亚细胞定位：
    Periplasm. Note=It is exported via the Tat pathway.
  • 蛋白家族：
    Multicopper oxidase family
  • 数据库链接：

    KEGG: ecj:JW0119

    STRING: 316385.ECDH10B_0103