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  4. Recombinant Escherichia coli Flagellar transcriptional regulator FlhD (flhD)

Recombinant Escherichia coli Flagellar transcriptional regulator FlhD (flhD)

  • 中文名称：
    大肠杆菌flhD重组蛋白
  • 货号：
    CSB-YP359186ENV
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    大肠杆菌flhD重组蛋白
  • 货号：
    CSB-EP359186ENV
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    大肠杆菌flhD重组蛋白
  • 货号：
    CSB-EP359186ENV-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    大肠杆菌flhD重组蛋白
  • 货号：
    CSB-BP359186ENV
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    大肠杆菌flhD重组蛋白
  • 货号：
    CSB-MP359186ENV
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    flhD
  • Uniprot No.：
  • 别名：
    flhD; flbB; b1892; JW1881Flagellar transcriptional regulator FlhD
  • 种属：
    Escherichia coli (strain K12)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-116
  • 氨基酸序列
    MHTSELLKHI YDINLSYLLL AQRLIVQDKA SAMFRLGINE EMATTLAALT LPQMVKLAET NQLVCHFRFD SHQTITQLTQ DSRVDDLQQI HTGIMLSTRL LNDVNQPEEA LRKKRA
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Functions in complex with FlhC as a master transcriptional regulator that regulates transcription of several flagellar and non-flagellar operons by binding to their promoter region. Activates expression of class 2 flagellar genes, including fliA, which is a flagellum-specific sigma factor that turns on the class 3 genes. Also regulates genes whose products function in a variety of physiological pathways.
  • 基因功能参考文献：
    1. three IS elements, IS1, IS3 and IS5, transpose to multiple upstream sites within a 370 bp region of the flhDC operon control region. The data are consistent with the possibility that appropriate environmental conditions, namely those that permit but hinder flagellar rotation, result in the activation of a mutational pathway that involves IS element insertion upstream of the flhDC operon. PMID: 28100305
    2. Mutations in the flhD can exert positive or negative effects on motility. PMID: 27821046
    3. FlhD is part of flagellar regulatory network. PMID: 25275371
    4. Upregulation of fliZ by NtrC-sigma(N) was shown to be indirect and required an intact flagellar regulator flhDC. PMID: 24931910
    5. s report that not only integrations of IS insertion sequences into the regulatory region of the flhDC operon, but also a missense mutation in the lrhA gene enhances motility by relieving transcriptional repression of the flhDC operon. PMID: 23456724
    6. CsrA activates flhDC expression by protecting flhDC mRNA from RNase E-mediated cleavage. PMID: 23305111
    7. Study demonstrates that insertion sequence IS5 in Escherichia coli inserts into the upstream region of the flhDC operon in a manner that depends on whether the environment permits motility. PMID: 21390082
    8. Mutations in the flhD do not affect cell division. PMID: 20546312
    9. These results indicate that the accumulation of sigmaS is one of the causes of the repression of transcription of flhD. PMID: 20185506
    10. IS element insertion may activate transcription of the flhD operon by reducing transcriptional repression. PMID: 15516564
    11. The data indicate a wider FlhD(2)C(2) regulon, in which non-flagellar genes are bound and activated directly PMID: 15941987
    12. flhDC mutants of Escherichia coli are nonmotile, better colonize in the intestine, and better utilize sugars than wild type bacteria and Entner-Doudoroff pathway mutants. PMID: 16299298
    13. crystallographic structure of the Escherichia coli FlhDC complex has been solved to 3.0 A resolution PMID: 16337229

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    FlhD family
  • 数据库链接：

    KEGG: ecj:JW1881

    STRING: 316385.ECDH10B_2033