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  4. Recombinant Escherichia coli GTPase ObgE/CgtA (obgE)

Recombinant Escherichia coli GTPase ObgE/CgtA (obgE)

  • 中文名称：
    大肠杆菌obgE重组蛋白
  • 货号：
    CSB-YP331590ENV
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    大肠杆菌obgE重组蛋白
  • 货号：
    CSB-EP331590ENV
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    大肠杆菌obgE重组蛋白
  • 货号：
    CSB-EP331590ENV-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    大肠杆菌obgE重组蛋白
  • 货号：
    CSB-BP331590ENV
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    大肠杆菌obgE重组蛋白
  • 货号：
    CSB-MP331590ENV
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    obgE
  • Uniprot No.：
  • 别名：
    obgE; cgtA; obg; yhbZ; b3183; JW3150; GTPase ObgE/CgtA; EC 3.6.5.-; GTP-binding protein Obg
  • 种属：
    Escherichia coli (strain K12)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-390
  • 氨基酸序列
    MKFVDEASIL VVAGDGGNGC VSFRREKYIP KGGPDGGDGG DGGDVWMEAD ENLNTLIDYR FEKSFRAERG QNGASRDCTG KRGKDVTIKV PVGTRVIDQG TGETMGDMTK HGQRLLVAKG GWHGLGNTRF KSSVNRTPRQ KTNGTPGDKR ELLLELMLLA DVGMLGMPNA GKSTFIRAVS AAKPKVADYP FTTLVPSLGV VRMDNEKSFV VADIPGLIEG AAEGAGLGIR FLKHLERCRV LLHLIDIDPI DGTDPVENAR IIISELEKYS QDLATKPRWL VFNKIDLLDK VEAEEKAKAI AEALGWEDKY YLISAASGLG VKDLCWDVMT FIIENPVVQA EEAKQPEKVE FMWDDYHRQQ LEEIAEEDDE DWDDDWDEDD EEGVEFIYKR
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    An abundant, essential GTPase which binds GTP, GDP and ppGpp with moderate affinity. Has high guanosine nucleotide exchange rate constants for GTP and GDP, and a relatively low GTP hydrolysis rate stimulated by the 50S ribosomal subunit. It is estimated there are 34000 molecules in log-phase cells and 5600 molecules in stationary-phase cells. Required for chromosome segregation. Plays a role in the stringent response, perhaps by sequestering 50S ribosomal subunits and decreasing protein synthesis, and a non-essential role in the late steps of ribosome biogenesis, perhaps acting as a checkpoint for correct 50S subunit synthesis (Probable). Overexpression increases bacterial persistence (in exponential and stationary phase) in response to antibiotics. Cells expressing high levels of Obg are more likely to form persister cells upon antibiotic exposure; this requires alarmone (p)ppGpp and acts via induced HokB, depolarizing cells, which probably reduces metabolic activity and induces persistence. The persister phenotype can be separated from the essential phenotype.; Required for correct chromosome partitioning; in temperature-sensitive (ts) mutant nucleoids do not partition but remain in the middle of cell, cells elongate but do not divide. Overexpression protects cells against UV damage. Ts mutants have impaired plasmid and lamdba phage replication, possibly via effects on DnaA. Regulates DnaA levels. Genetic interactions of Val-168 and a C-terminal insertion mutant with the double-strand break repair factors recA and recBCD, and with seqA suggests that ObgE, either directly or indirectly, promotes replication fork stability. May protect replication forks from stress induced by toxic levels of hydroxyl radicals. Initiation of DNA replication continues in ObgE-depleted cells.; Binds to pre-50S ribosomal subunits in a salt-dependent manner. Acts as a ribosome anti-association factor; guanosine nucleotides stimulate ObgE 50S subunit binding and also ObgE-mediated 70S ribosome dissociation (GDP
  • 基因功能参考文献：
    1. Biophysical analysis, using small angle X-ray scattering and multi-angle light scattering experiments, revealed that ObgE is a monomer in solution, regardless of the bound nucleotide. In contrast to recent suggestions, our biochemical analyses further indicate that ObgE is neither activated by K(+) ions nor by homodimerization. PMID: 28223358
    2. The s report that expression of a mutant isoform of the essential GTPase ObgE causes rapid loss of viability in Escherichia coli. PMID: 26695632
    3. ObgE might act as a checkpoint in final stages of the 50S subunit assembly under normal growth conditions, and, as a (p)ppGpp effector, might also have a regulatory role in the production of the 50S subunit under stressed conditions. PMID: 24844575
    4. In this study we apply a new semiquantitative proteomic approach to show that CgtA(E) is required for optimal incorporation of certain late-assembly ribosomal proteins into the large ribosomal subunit. PMID: 16980477
    5. ObgE is required to license chromosome segregation and subsequent cell cycle events. PMID: 17578452
    6. Study proposes that, in addition to its role as a 50S assembly factor, CgtA promotes SpoT (p)ppGpp degradation activity on the ribosome and that the loss of CgtA from the ribosome is necessary for maximal (p)ppGpp accumulation under stress conditions. PMID: 17616600
    7. ObgE changes the ratio of ppGpp to pppGpp in the cell during a response to amino acid starvation. PMID: 19555460

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    TRAFAC class OBG-HflX-like GTPase superfamily, OBG GTPase family
  • 数据库链接：

    KEGG: ecj:JW3150

    STRING: 316385.ECDH10B_3357