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  4. Recombinant Escherichia coli HTH-type transcriptional regulator YjiE (yjiE)

Recombinant Escherichia coli HTH-type transcriptional regulator YjiE (yjiE)

  • 中文名称：
    大肠杆菌yjiE重组蛋白
  • 货号：
    CSB-YP340088ENV
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    大肠杆菌yjiE重组蛋白
  • 货号：
    CSB-EP340088ENV
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    大肠杆菌yjiE重组蛋白
  • 货号：
    CSB-EP340088ENV-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    大肠杆菌yjiE重组蛋白
  • 货号：
    CSB-BP340088ENV
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    大肠杆菌yjiE重组蛋白
  • 货号：
    CSB-MP340088ENV
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    yjiE
  • Uniprot No.：
  • 别名：
    yjiE; qseD; b4327; JW4290; HTH-type transcriptional regulator YjiE; Hypochlorite-response regulator protein YjiE; Quorum-sensing regulator protein D
  • 种属：
    Escherichia coli (strain K12)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-303
  • 氨基酸序列
    MDDCGAILHN IETKWLYDFL TLEKCRNFSQ AAVSRNVSQP AFSRRIRALE QAIGVELFNR QVTPLQLSEQ GKIFHSQIRH LLQQLESNLA ELRGGSDYAQ RKIKIAAAHS LSLGLLPSII SQMPPLFTWA IEAIDVDEAV DKLREGQSDC IFSFHDEDLL EAPFDHIRLF ESQLFPVCAS DEHGEALFNL AQPHFPLLNY SRNSYMGRLI NRTLTRHSEL SFSTFFVSSM SELLKQVALD GCGIAWLPEY AIQQEIRSGK LVVLNRDELV IPIQAYAYRM NTRMNPVAER FWRELRELEI VLS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Protects cells from HOCl (hypochlorite) stress but not peroxide or diamide stress. Decreases the intracellular load of reactive oxygen species by up-regulating genes involved in methionine and cysteine biosynthesis and down-regulating Fur-regulated genes involved in iron acquisition. Has also been suggested to down-regulate expression of the flagellar regulon, decreasing motility, but this activity was not confirmed in a second study.
  • 基因功能参考文献：
    1. Data indicate that YjiE renamed HypT (hypochlorite-responsive transcription factor) oligomers are the activation-competent species, whereas the dodecamers are a storage form. PMID: 24275662
    2. findings show that YjiE is the first HOCl-specific transcription factor that increases cellular viability by reducing the intracellular iron levels PMID: 22223481
  • 数据库链接：

    KEGG: ecj:JW4290

    STRING: 316407.85677070