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  4. Recombinant Escherichia coli Maltoporin (lamB), partial

Recombinant Escherichia coli Maltoporin (lamB), partial

  • 中文名称：
    大肠杆菌lamB重组蛋白
  • 货号：
    CSB-YP365810ENV
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    大肠杆菌lamB重组蛋白
  • 货号：
    CSB-EP365810ENV
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    大肠杆菌lamB重组蛋白
  • 货号：
    CSB-EP365810ENV-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    大肠杆菌lamB重组蛋白
  • 货号：
    CSB-BP365810ENV
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    大肠杆菌lamB重组蛋白
  • 货号：
    CSB-MP365810ENV
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    lamB
  • Uniprot No.：
  • 别名：
    lamB; malB; b4036; JW3996; Maltoporin; Lambda receptor protein; Maltose-inducible porin
  • 种属：
    Escherichia coli (strain K12)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Involved in the transport of maltose and maltodextrins. Indispensable for translocation of maltodextrins (alpha 1-4 linked polyglucosyls) containing more than three glucosyl moieties. A hydrophobic path ('greasy slide') of aromatic residues serves to guide and select the sugars for transport through the channel. Also acts as a receptor for several bacteriophages including lambda. Binds maltosaccharides; when LamB binds starch in soft agar, it inhibits motility.
  • 基因功能参考文献：
    1. Our data indicate that the negative regulation by LamB is widely detected in antibiotic-resistant E. coli. LamB serves as a porin to influx an antibiotic and is interacted with Odp1. PMID: 24412198
    2. Results report the crystallization of E. coli maltoporin in a new crystal form; the crystals belonged to the trigonal R3 space group and diffracted to 1.9 A resolution. PMID: 21206039
    3. downregulation of LamB expression in the CTC-resistant E. coli strain and the increased antibiotic resistance in its gene-deleted strain suggested a negative regulation mechanism in E. coli in response to CTC PMID: 20718490
    4. demonstrated that LamB moves along the bacterial surface and that these movements are restricted by an underlying dynamic spiral pattern PMID: 15341654
    5. all aromatic residues provide some steric hindrance for ion transport through LamB PMID: 16095613

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  • 亚细胞定位：
    Cell outer membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Porin LamB (TC 1.B.3) family
  • 数据库链接：

    KEGG: ecj:JW3996

    STRING: 316385.ECDH10B_4225