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  4. Recombinant Escherichia coli Purine nucleoside phosphorylase DeoD-type (deoD)

Recombinant Escherichia coli Purine nucleoside phosphorylase DeoD-type (deoD)

  • 中文名称：
    Recombinant Escherichia coli Purine nucleoside phosphorylase DeoD-type(deoD)
  • 货号：
    CSB-EP364680ENVe0
  • 规格：
    ￥2328
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    deoD
  • Uniprot No.：
  • 别名：
    deoD; pup; b4384; JW4347; Purine nucleoside phosphorylase DeoD-type; PNP; EC 2.4.2.1; Inosine phosphorylase
  • 种属：
    Escherichia coli (strain K12)
  • 蛋白长度：
    Full Length of Mature Protein
  • 来源：
    E.coli
  • 分子量：
    52.8kDa
  • 表达区域：
    2-239aa
  • 氨基酸序列
    ATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVRGMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLRDVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLFYSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAERQTTFNDMIKIALESVLLGDKE
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal GST-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalyzes the reversible phosphorolytic breakdown of the N-glycosidic bond in the beta-(deoxy)ribonucleoside molecules, with the formation of the corresponding free purine bases and pentose-1-phosphate. Acts on 6-amino and 6-oxopurines including deoxyinosine, deoxyguanosine, deoxyadenosine, adenosine, guanosine, and inosine. Does not act on xanthosine. May also catalyze a phosphate-dependent transfer of the pentose moiety from one purine base to another.
  • 基因功能参考文献：
    1. These studies also indicate an important role of the phenyl ring of Phe159 for FRET in the wild-type purine nucleoside phosphorylase, which does not exist in the Ala159 mutant, and for the effective association of PNP with formycin A . PMID: 25754135
    2. Data suggest that in active sites of purine nucleoside phosphorylase complexed with phosphate and formycin A, recombinant enzyme exhibits unexpected binding site for phosphate and exhibits stoichiometry of two phosphate molecules per enzyme subunit. PMID: 22569248
    3. The deoD gene was cloned from Escherichia coli MG1665 & overexpressed in E. coli BL 21(DE3). PMID: 22113014
    4. in solution similar to the crystal, E. coli PNP is a hexameric molecule and previous suggestions for coexistence of two oligomeric forms are incorrect. PMID: 16751611
    5. Role of ionization of the phosphate cosubstrate on phosphorolysis by purine nucleoside phosphorylase (PNP) PMID: 17639373

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  • 蛋白家族：
    PNP/UDP phosphorylase family
  • 数据库链接：

    KEGG: ecj:JW4347

    STRING: 316385.ECDH10B_4542