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  4. Recombinant Escherichia coli Purine nucleoside phosphorylase DeoD-type (deoD)

Recombinant Escherichia coli Purine nucleoside phosphorylase DeoD-type (deoD)

  • 中文名称：
    Recombinant Escherichia coli Purine nucleoside phosphorylase DeoD-type(deoD),Yeast
  • 货号：
    CSB-YP364680ENV
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Escherichia coli Purine nucleoside phosphorylase DeoD-type(deoD),Yeast
  • 货号：
    CSB-EP364680ENV-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Escherichia coli Purine nucleoside phosphorylase DeoD-type(deoD),Yeast
  • 货号：
    CSB-BP364680ENV
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Escherichia coli Purine nucleoside phosphorylase DeoD-type(deoD),Yeast
  • 货号：
    CSB-MP364680ENV
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    deoD
  • Uniprot No.：
  • 别名：
    deoD; pup; b4384; JW4347; Purine nucleoside phosphorylase DeoD-type; PNP; EC 2.4.2.1; Inosine phosphorylase
  • 种属：
    Escherichia coli (strain K12)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-239
  • 氨基酸序列
    ATPHINAEM GDFADVVLMP GDPLRAKYIA ETFLEDAREV NNVRGMLGFT GTYKGRKISV MGHGMGIPSC SIYTKELITD FGVKKIIRVG SCGAVLPHVK LRDVVIGMGA CTDSKVNRIR FKDHDFAAIA DFDMVRNAVD AAKALGIDAR VGNLFSADLF YSPDGEMFDV MEKYGILGVE MEAAGIYGVA AEFGAKALTI CTVSDHIRTH EQTTAAERQT TFNDMIKIAL ESVLLGDKE
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalyzes the reversible phosphorolytic breakdown of the N-glycosidic bond in the beta-(deoxy)ribonucleoside molecules, with the formation of the corresponding free purine bases and pentose-1-phosphate. Acts on 6-amino and 6-oxopurines including deoxyinosine, deoxyguanosine, deoxyadenosine, adenosine, guanosine, and inosine. Does not act on xanthosine. May also catalyze a phosphate-dependent transfer of the pentose moiety from one purine base to another.
  • 基因功能参考文献：
    1. These studies also indicate an important role of the phenyl ring of Phe159 for FRET in the wild-type purine nucleoside phosphorylase, which does not exist in the Ala159 mutant, and for the effective association of PNP with formycin A . PMID: 25754135
    2. Data suggest that in active sites of purine nucleoside phosphorylase complexed with phosphate and formycin A, recombinant enzyme exhibits unexpected binding site for phosphate and exhibits stoichiometry of two phosphate molecules per enzyme subunit. PMID: 22569248
    3. The deoD gene was cloned from Escherichia coli MG1665 & overexpressed in E. coli BL 21(DE3). PMID: 22113014
    4. in solution similar to the crystal, E. coli PNP is a hexameric molecule and previous suggestions for coexistence of two oligomeric forms are incorrect. PMID: 16751611
    5. Role of ionization of the phosphate cosubstrate on phosphorolysis by purine nucleoside phosphorylase (PNP) PMID: 17639373

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  • 蛋白家族：
    PNP/UDP phosphorylase family
  • 数据库链接：

    KEGG: ecj:JW4347

    STRING: 316385.ECDH10B_4542