Your Good Partner in Biology Research

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Escherichia coli Thiol:disulfide interchange protein DsbD (dsbD), partial

Recombinant Escherichia coli Thiol:disulfide interchange protein DsbD (dsbD), partial

  • 中文名称：
    大肠杆菌dsbD重组蛋白
  • 货号：
    CSB-YP327569ENV
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    大肠杆菌dsbD重组蛋白
  • 货号：
    CSB-EP327569ENV
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    大肠杆菌dsbD重组蛋白
  • 货号：
    CSB-EP327569ENV-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    大肠杆菌dsbD重组蛋白
  • 货号：
    CSB-BP327569ENV
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    大肠杆菌dsbD重组蛋白
  • 货号：
    CSB-MP327569ENV
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    dsbD
  • Uniprot No.：
  • 别名：
    dsbD; cutA2; cycZ; dipZ; b4136; JW5734Thiol:disulfide interchange protein DsbD; EC 1.8.1.8; C-type cytochrome biogenesis protein CycZ; Inner membrane copper tolerance protein; Protein-disulfide reductase; Disulfide reductase
  • 种属：
    Escherichia coli (strain K12)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Required to facilitate the formation of correct disulfide bonds in some periplasmic proteins and for the assembly of the periplasmic c-type cytochromes. Acts by transferring electrons from cytoplasmic thioredoxin to the periplasm, thereby maintaining the active site of DsbC, DsbE and DsbG in a reduced state. This transfer involves a cascade of disulfide bond formation and reduction steps.
  • 基因功能参考文献：
    1. The s show that the F plasmid-encoded TrbB relies on DsbD from Escherichia coli for maintenance of its C-X-X-C redox active site motif. PMID: 21742866
    2. Cys109 and Cys461 form a transient interdomain disulfide bond between nDsbD and cDsbD in the reaction cycle of DsbD PMID: 15057279
    3. kinetic analysis of DsbD-dependent cytochrome c maturation PMID: 16004871
    4. N-terminal (nDsbD) and a C-terminal (cDsbD) are likely to be correlated to the role of cDsbD as a covalently linked electron shuttle between the membrane domain and the N-terminal periplasmic domain of DsbD PMID: 16545842
    5. The structural features enabling nDsbD, a 'redox hub' with an immunoglobulin-like fold, to interact efficiently with its different thioredoxin-like partners are reviewed [review] PMID: 16786221
    6. Properties of the mutants to the positions of the amino acids in the structure of DsbD and discuss mechanisms that would interfere with the electron transfer process. PMID: 16816179
    7. Here we provide evidence that DsbD's conformation depends on its oxidation state. PMID: 17015672
    8. pK(a) value of the second cysteine in wild-type cDsbD, Cys464, is significantly higher than the maximum pH value that was studied PMID: 17544440
    9. analysis of the structure and function of DsbD PMID: 19258316

    显示更多

    收起更多

  • 亚细胞定位：
    Cell inner membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Thioredoxin family, DsbD subfamily
  • 数据库链接：

    KEGG: ecj:JW5734

    STRING: 316385.ECDH10B_4329