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  4. Recombinant Escherichia coli Uncharacterized protein yejH (yejH)

Recombinant Escherichia coli Uncharacterized protein yejH (yejH)

  • 中文名称：
    大肠杆菌radD重组蛋白
  • 货号：
    CSB-YP327272ENV
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    大肠杆菌radD重组蛋白
  • 货号：
    CSB-EP327272ENV
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    大肠杆菌radD重组蛋白
  • 货号：
    CSB-EP327272ENV-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    大肠杆菌radD重组蛋白
  • 货号：
    CSB-BP327272ENV
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    大肠杆菌radD重组蛋白
  • 货号：
    CSB-MP327272ENV
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    radD
  • Uniprot No.：
  • 别名：
    radD; yejH; yejI; yejJ; b2184; JW2172; Putative DNA repair helicase RadD; EC 3.6.4.12
  • 种属：
    Escherichia coli (strain K12)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-586
  • 氨基酸序列
    MIFTLRPYQQ EAVDATLNHF RRHKTPAVIV LPTGAGKSLV IAELARLARG RVLVLAHVKE LVAQNHAKYQ ALGLEADIFA AGLKRKESHG KVVFGSVQSV ARNLDAFQGE FSLLIVDECH RIGDDEESQY QQILTHLTKV NPHLRLLGLT ATPFRLGKGW IYQFHYHGMV RGDEKALFRD CIYELPLRYM IKHGYLTPPE RLDMPVVQYD FSRLQAQSNG LFSEADLNRE LKKQQRITPH IISQIMEFAA TRKGVMIFAA TVEHAKEIVG LLPAEDAALI TGDTPGAERD VLIENFKAQR FRYLVNVAVL TTGFDAPHVD LIAILRPTES VSLYQQIVGR GLRLAPGKTD CLILDYAGNP HDLYAPEVGT PKGKSDNVPV QVFCPACGFA NTFWGKTTAD GTLIEHFGRR CQGWFEDDDG HREQCDFRFR FKNCPQCNAE NDIAARRCRE CDTVLVDPDD MLKAALRLKD ALVLRCSGMS LQHGHDEKGE WLKITYYDED GADVSERFRL QTPAQRTAFE QLFIRPHTRT PGIPLRWITA ADILAQQALL RHPDFVVARM KGQYWQVREK VFDYEGRFRL AHELRG
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    RadD contains helicase motifs, suggesting it may be a helicase, although that activity has not been observed (Probable). In combination with RadA is important in repair of double-strand DNA breaks (DSB). Has DNA-independent ATPase activity that is stimulated by single-stranded DNA-binding protein SSB. ATPase is stimulated by a peptide with the last 10 residues of SSB, but not when the peptide's last Phe residue is missing. Binds ssDNA; binding is slightly better in the presence of nucleotides. May be involved in resolution of branched DNA intermediates that result from template switching in postreplication gaps. Binds to DNA structures with 3 branches that resemble replication forks.
  • 基因功能参考文献：
    1. it is likely that the repair function of RadD is mediated through interaction with SSB at the site of damage. PMID: 27519413
    2. investigation of the function of yejH; established a role for yejH gene product in repair of double-strand breaks that largely overlaps that of the genes radA and recG; propose yejH be renamed radD, reflecting its role in DNA repair of radiation damage PMID: 25425430
  • 数据库链接：

    KEGG: ecj:JW2172

    STRING: 316385.ECDH10B_2342