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  4. Recombinant Human 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase (ADI1)

Recombinant Human 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase (ADI1)

  • 中文名称：
    Recombinant Human 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase(ADI1),Yeast
  • 货号：
    CSB-YP861145HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Human 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase(ADI1),Yeast
  • 货号：
    CSB-EP861145HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Human 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase(ADI1),Yeast
  • 货号：
    CSB-EP861145HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Human 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase(ADI1),Yeast
  • 货号：
    CSB-BP861145HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Human 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase(ADI1),Yeast
  • 货号：
    CSB-MP861145HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    ADI1
  • Uniprot No.：
  • 别名：
    1 2 dihydroxy 3 keto 5 methylthiopentene dioxygenase; 1; 2-dihydroxy-3-keto-5-methylthiopentene dioxygenase; Aci reductone dioxygenase; Acireductone dioxygenase (Fe(2+) requiring); Acireductone dioxygenase (Ni(2+) requiring); Acireductone dioxygenase (Ni(2+)-requiring); Acireductone dioxygenase 1; ADI 1; Adi1; ADI1; APL1; ARD; EC 1.13.; Fe ARD; FLJ10913; HMFT1638; Membrane type 1 matrix metalloproteinase cytoplasmic tail binding protein 1; Membrane-type 1 matrix metalloproteinase cytoplasmic tail-binding protein 1; MT1 MMP cytoplasmic tail binding protein 1; MTCBP 1; MTCBP-1; MTCBP1; MTND_HUMAN; Ni-ARD; SIPL; Submergence induced protein 2; Submergence induced protein 2 homolog; Submergence induced protein like factor; Submergence-induced protein 2 homolog
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-179
  • 氨基酸序列
    MVQAWYMDDA PGDPRQPHRP DPGRPVGLEQ LRRLGVLYWK LDADKYENDP ELEKIRRERN YSWMDIITIC KDKLPNYEEK IKMFYEEHLH LDDEIRYILD GSGYFDVRDK EDQWIRIFME KGDMVTLPAG IYHRFTVDEK NYTKAMRLFV GEPVWTAYNR PADHFEARGQ YVKFLAQTA
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalyzes the formation of formate and 2-keto-4-methylthiobutyrate (KMTB) from 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene). Also down-regulates cell migration mediated by MMP14. Necessary for hepatitis C virus replication in an otherwise non-permissive cell line.
  • 基因功能参考文献：
    1. High ADI1 expression is associated with malignant glioma. PMID: 30066900
    2. The thermal stability of the HsARD isozymes is a function of the metal ion identity, with Ni2+-bound HsARD being the most stable followed by Co2+ and Fe2+, and Mn2+-bound HsARD being the least stable. PMID: 28062648
    3. In summary, clinical and cell-based experiments suggested that physical interaction between MT1-MMP and ADI1 led to suppression of hepatitis C virus infection. This inhibitory effect could be reversed by ADI1 overexpression. PMID: 26537061
    4. Data elucidate a new role for MTCBP-1 regulating the intracellular function of MT1-MMP-mediated autophagy. Their inverse expression correlation with brain tumor grades could also contribute to the decreased autophagic index observed in high-grade tumors. PMID: 25640948
    5. MTCBP-1 is a new member of the Cupin superfamily with a role as an invasion suppressor down-regulated in tumors PMID: 14718544
    6. nucleo-cytoplasmic transport of hADI1 is regulated by a non-canonical nuclear export signal (NES) located in the N-terminal region of hADI1. PMID: 17212658
    7. ADI1 may check prostate cancer progression through apoptosis by an activity that does not require metal binding. PMID: 17786183
    8. 293-ADI1-CD81 cells are permissive for serum-derived HCV infection. PMID: 19626614

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  • 亚细胞定位：
    Cytoplasm. Nucleus. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Note=Localizes to the plasma membrane when complexed to MMP14.
  • 蛋白家族：
    Acireductone dioxygenase (ARD) family
  • 组织特异性：
    Detected in heart, colon, lung, stomach, brain, spleen, liver, skeletal muscle and kidney.
  • 数据库链接：

    HGNC: 30576

    OMIM: 613400

    KEGG: hsa:55256

    STRING: 9606.ENSP00000333666

    UniGene: Hs.502773