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  4. Recombinant Human Collagen type IV alpha-3-binding protein (COL4A3BP)

Recombinant Human Collagen type IV alpha-3-binding protein (COL4A3BP)

  • 中文名称：
    Recombinant Human Collagen type IV alpha-3-binding protein(COL4A3BP),Yeast
  • 货号：
    CSB-YP896912HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Human Collagen type IV alpha-3-binding protein(COL4A3BP),Yeast
  • 货号：
    CSB-EP896912HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Human Collagen type IV alpha-3-binding protein(COL4A3BP),Yeast
  • 货号：
    CSB-EP896912HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Human Collagen type IV alpha-3-binding protein(COL4A3BP),Yeast
  • 货号：
    CSB-BP896912HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Human Collagen type IV alpha-3-binding protein(COL4A3BP),Yeast
  • 货号：
    CSB-MP896912HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    COL4A3BP
  • Uniprot No.：
  • 别名：
    Alpha 3 type IV collagen binding protein; C43BP_HUMAN; Ceramide transfer protein; Ceramide transporter; CERT; Cert protein; CERTL; col4a3bp; Collagen type IV alpha 3 (Goodpasture antigen) binding protein; Collagen type IV alpha 3 binding protein; Collagen type IV alpha-3-binding protein; FLJ20597; Goodpasture antigen binding protein; Goodpasture antigen-binding protein; GPBP; hCERT; Lipid transfer protein CERTL; Procollagen type IV alpha 3 (Goodpasture antigen) binding protein; StAR related lipid transfer (START) domain containing 11; StAR related lipid transfer protein 11; StAR-related lipid transfer protein 11; StARD 11; StARD11; START domain containing 11; START domain containing protein 11; START domain-containing protein 11
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-624
  • 氨基酸序列
    MSDNQSWNSS GSEEDPETES GPPVERCGVL SKWTNYIHGW QDRWVVLKNN ALSYYKSEDE TEYGCRGSIC LSKAVITPHD FDECRFDISV NDSVWYLRAQ DPDHRQQWID AIEQHKTESG YGSESSLRRH GSMVSLVSGA SGYSATSTSS FKKGHSLREK LAEMETFRDI LCRQVDTLQK YFDACADAVS KDELQRDKVV EDDEDDFPTT RSDGDFLHST NGNKEKLFPH VTPKGINGID FKGEAITFKA TTAGILATLS HCIELMVKRE DSWQKRLDKE TEKKRRTEEA YKNAMTELKK KSHFGGPDYE EGPNSLINEE EFFDAVEAAL DRQDKIEEQS QSEKVRLHWP TSLPSGDAFS SVGTHRFVQK PYSRSSSMSS IDLVSASDDV HRFSSQVEEM VQNHMTYSLQ DVGGDANWQL VVEEGEMKVY RREVEENGIV LDPLKATHAV KGVTGHEVCN YFWNVDVRND WETTIENFHV VETLADNAII IYQTHKRVWP ASQRDVLYLS VIRKIPALTE NDPETWIVCN FSVDHDSAPL NNRCVRAKIN VAMICQTLVS PPEGNQEISR DNILCKITYV ANVNPGGWAP ASVLRAVAKR EYPKFLKRFT SYVQEKTAGK PILF
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Shelters ceramides and diacylglycerol lipids inside its START domain and mediates the intracellular trafficking of ceramides and diacylglycerol lipids in a non-vesicular manner.
  • 基因功能参考文献：
    1. Importance of Endoplasmic reticulum to Golgi ceramide traffic to preserve muscle cell insulin signaling, CERT as a major actor in this process. PMID: 29759974
    2. Data suggest that isolated PH and START domains of CERT interact with each other; START domain binds to PH domain at same site for PtdIns[4]P-binding; mutations disrupting PH-START interaction increase both PtdIns[4]P-binding affinity and ceramide transfer activity. (PH domain = N-terminal pleckstrin homology domain; START domain = StAR-related lipid transfer domain; PtdIns[4]P = phosphatidylinositol 4-phosphate) PMID: 28652409
    3. Cells expressing mitoCERT import ceramides into mitochondria and undergo Bax-dependent apoptosis. PMID: 27888218
    4. phosphorylation of CERT at the FFAT motif-adjacent serine affects its affinity for VAP, which may regulate the inter-organelle trafficking of ceramide in response to the perturbation of cellular sphingomyelin and/or other sphingolipids PMID: 24569996
    5. Both CERT isoforms, when immobilized, bind the globular head region of C1q and initiate the classical complement pathway. C1q binds endogenous CERTL on the surface of apoptotic cells. PMID: 24395916
    6. Structural basis for the Golgi association by the pleckstrin homology domain of the ceramide trafficking protein (CERT) PMID: 22869376
    7. CERT is at a convergence point of non-vesicular and vesicular transport processes and plays a central role within the PKD signaling network at the Golgi complex. (Review) PMID: 22226883
    8. Loss of the ceramide transfer protein augments EGF receptor signaling in breast cancer. PMID: 22472120
    9. The START domain in GPBP is important for this interaction. SAP and GPBP form complexes in blood and partly colocalize in amyloid plaques from Alzheimer disease patients. PMID: 22396542
    10. This review is focused on ceramide transfer protein (CERT) as a major regulator of ceramide flux in the cell. [review] PMID: 21707482
    11. during cellular stress, disassembly of Golgi structure together with inactivation of CERT by caspases causes a reduction in ceramide trafficking and sphingomyelin synthesis, and could contribute to the cellular response to pro-apoptotic stress PMID: 22129459
    12. the presence of IncD, CERT, VAPA/B, and potentially additional host and/or bacterial factors, at points of contact between the ER and the inclusion membrane provides a specialized microenvironment favorable to bacterial development. PMID: 21731489
    13. We hypothesize that sphingomyelin acquired by CERT-dependent transport of ceramide and subsequent conversion to SM is necessary for C. trachomatis replication whereas SM acquired by GBF1-dependent pathway is essential for inclusion growth and stability. PMID: 21909260
    14. GPBP directs myofibril formation through interaction with intracellular downstream effector 130-kDa GPBP-interacting protein PMID: 21832087
    15. PKD negatively regulates HCV secretion/release by attenuating OSBP and CERT functions by phosphorylation inhibition. This study identifies the key role of the Golgi components in the HCV maturation process. PMID: 21285358
    16. the membrane matrix surrounding ceramide, that is ceramide miscibility, is largely affecting the transfer activity of CERT PMID: 20875392
    17. hBVR is a regulator of the TNF-alpha-GPBP-collagen type IV signaling cascade PMID: 20177069
    18. Crystal structures of the CERT START domain in complex with HPAs ((1R,3R)-N-(3-Hydroxy-1-hydroxymethyl-3-phenylpropyl)alkanamide) of varying acyl chain lengths. PMID: 20036255
    19. CERT mediates the intracellular trafficking of ceramide in a non-vesicular manner PMID: 14685229
    20. results indicate that CERT can mediate transfer of various types of ceramides that naturally exist and their close relatives PMID: 15596449
    21. crypticity is a feature of the quaternary structure of two subsets of alpha3alpha4alpha5(IV) NC1 hexamers: autoantibody-reactive M-hexamers containing only monomer subunits and autoantibody-impenetrable D-hexamers composed of dimer and monomer subunits PMID: 15917228
    22. Results identify a human-specific TNF-responsive transcriptional unit that locates GPBP in the signalling cascade of TNF and substantiates previous observations, which independently related TNF and GPBP with human autoimmunity. PMID: 16218959
    23. A clinical evaluation of probands and their relatives of the five families carrying mutations in either the COL4A3 or the COL4A4 gene was carried out to underline the natural history of the autosomal recessive ATS. PMID: 16338941
    24. Regulation of ceramide transport protein by oxysterol binding protein, and VAMP reveals a novel mechanism for ceramide transport and sphingophingomyelin synthesis in the Golgi apparatus. PMID: 16571669
    25. The Golgi-targeting domain and endoplasmic reticulum-interacting FFAT amino acid motif of CERT spatially restrict the random ceramide transfer activity of the START domain in cells. PMID: 16895911
    26. Data show that CERT receives multiple phosphorylations at a serine-repeat motif, a possibe site for casein kinase I, and that the phosphorylation down-regulates the ER-to-Golgi transport of ceramide. PMID: 17442665
    27. Regulation of secretory transport by PKD-mediated phosphorylation of CERT is reported. PMID: 17591919
    28. GPBP regulates GBM collagen organization and its elevated expression causes dissociation and subsequent accumulation of IgA on the GBM. PMID: 17916599
    29. CERT is a physiological substrate of PP2Cepsilon and that dephosphorylation of CERT by PP2Cepsilon may play an important role in the regulation of ceramide trafficking from the ER to the Golgi apparatus. PMID: 18165232
    30. analysis of the mechanism by which CERT can distinguish ceramide from other lipid types yet still recognize multiple species of ceramides PMID: 18184806
    31. healthy individuals have low frequencies of unstimulated alpha3(IV)NC1-reactive T cells with similar specificities to the autoreactive T cells found in patients with Goodpasture disease PMID: 18216317
    32. CERT can attenuate a key metabolic protective mechanism against ceramide-induced apoptosis in keratinocytes PMID: 18411267
    33. Goodpasture antigen-binding protein is a soluble exportable protein that interacts with type IV collagen PMID: 18772132
    34. These results indicate that CKIgamma2 hyperphosphorylates the serine-repeat motif of CERT, thereby inactivating CERT and down-regulating the synthesis of sphingomyelin. PMID: 19005213
    35. the allergen causing the most food-induced anaphylaxis in Italy PMID: 19494524

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  • 相关疾病：
    Mental retardation, autosomal dominant 34 (MRD34)
  • 亚细胞定位：
    Cytoplasm. Golgi apparatus. Endoplasmic reticulum.
  • 组织特异性：
    Widely expressed.
  • 数据库链接：

    HGNC: 2205

    OMIM: 604677

    KEGG: hsa:10087

    STRING: 9606.ENSP00000369862

    UniGene: Hs.270437