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  4. Recombinant Human E3 SUMO-protein ligase NSE2 (NSMCE2)

Recombinant Human E3 SUMO-protein ligase NSE2 (NSMCE2)

  • 中文名称：
    人NSMCE2重组蛋白
  • 货号：
    CSB-YP836260HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人NSMCE2重组蛋白
  • 货号：
    CSB-EP836260HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人NSMCE2重组蛋白
  • 货号：
    CSB-EP836260HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人NSMCE2重组蛋白
  • 货号：
    CSB-BP836260HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人NSMCE2重组蛋白
  • 货号：
    CSB-MP836260HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    NSMCE2
  • Uniprot No.：
  • 别名：
    C8orf36; E3 SUMO-protein ligase NSE2; hMMS21; hNSE2; methyl methanesulfonate sensitivity gene 21 ; MMS21; MMS21 homolog; non-SMC element 2; Non-SMC element 2 homolog; Non-structural maintenance of chromosomes element 2 homolog; NSE2; NSE2_HUMAN; Nsmce2
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-247
  • 氨基酸序列
    MPGRSSSNSG STGFISFSGV ESALSSLKNF QACINSGMDT ASSVALDLVE SQTEVSSEYS MDKAMVEFAT LDRQLNHYVK AVQSTINHVK EERPEKIPDL KLLVEKKFLA LQSKNSDADF QNNEKFVQFK QQLKELKKQC GLQADREADG TEGVDEDIIV TQSQTNFTCP ITKEEMKKPV KNKVCGHTYE EDAIVRMIES RQKRKKKAYC PQIGCSHTDI RKSDLIQDEA LRRAIENHNK KRHRHSE
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    E3 SUMO-protein ligase component of the SMC5-SMC6 complex, a complex involved in DNA double-strand break repair by homologous recombination. Is not be required for the stability of the complex. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines and mediates sumoylation of shelterin complex (telosome) components which is proposed to lead to shelterin complex disassembly in ALT-associated PML bodies (APBs). Acts as an E3 ligase mediating SUMO attachment to various proteins such as SMC6L1 and TSNAX, the shelterin complex subunits TERF1, TERF2, TINF2 and TERF2IP, RAD51AP1, and maybe the cohesin components RAD21 and STAG2. Required for recruitment of telomeres to PML nuclear bodies. SUMO protein-ligase activity is required for the prevention of DNA damage-induced apoptosis by facilitating DNA repair, and for formation of APBs in ALT cell lines. Required for sister chromatid cohesion during prometaphase and mitotic progression.
  • 基因功能参考文献：
    1. Absence of NSMCE2 affects survival upon topoisomerase II inhibitor etoposide-induced DNA double-strand breaks double-strand breaks. PMID: 27792189
    2. our study identified novel PVT1-NSMCE2 and CCDC26-NSMCE2 fusion genes that may play functional roles in leukemia. PMID: 25245984
    3. support a role for NSMCE2 in recovery from DNA damage and raise the possibility that loss of its function produces dwarfism through reduced tolerance of replicative stress PMID: 25105364
    4. show that the Smc5/6 subunit Mms21 sumoylates multiple lysines of the cohesin subunit Scc1 PMID: 22751501
    5. Studies indicate that Nse1 may function as a ubiquitin ligase, and is targeted to chromatin through its interaction with the Smc5/6 complex. PMID: 21550342
    6. Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator) PMID: 20379614
    7. Observational study of gene-disease association. (HuGE Navigator) PMID: 20460480
    8. the human SMC5/6 complex and the SUMO ligase activity of hMMS21 are required for the prevention of DNA damage-induced apoptosis by facilitating DNA repair in human cells PMID: 16055714
    9. MMS21 dependent sumoylation is integral and important to the cohesion mechanism and mitotic progression and that this function appears to be independent of SMC6. PMID: 19502785
    10. Observational study of gene-disease association. (HuGE Navigator) PMID: 19549807
    11. Observational study of gene-disease association. (HuGE Navigator) PMID: 18163389

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  • 相关疾病：
    Seckel syndrome 10 (SCKL10)
  • 亚细胞定位：
    Nucleus. Chromosome, telomere. Nucleus, PML body.
  • 蛋白家族：
    NSE2 family
  • 数据库链接：

    HGNC: 26513

    OMIM: 617246

    KEGG: hsa:286053

    STRING: 9606.ENSP00000287437

    UniGene: Hs.388297