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  4. Recombinant Human E3 ubiquitin-protein ligase CHIP (STUB1)

Recombinant Human E3 ubiquitin-protein ligase CHIP (STUB1)

  • 中文名称：
    人STUB1重组蛋白
  • 货号：
    CSB-YP892480HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人STUB1重组蛋白
  • 货号：
    CSB-EP892480HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人STUB1重组蛋白
  • 货号：
    CSB-EP892480HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人STUB1重组蛋白
  • 货号：
    CSB-BP892480HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人STUB1重组蛋白
  • 货号：
    CSB-MP892480HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Antigen NY CO 7; Antigen NY-CO-7; C terminus of Hsp70-interacting protein; Carboxy terminus of Hsp70 interacting protein; Carboxy terminus of Hsp70-interacting protein; Carboxy terminus of Hsp70p interacting protein; CHIP; CHIP_HUMAN; CLL associated antigen KW 8; CLL-associated antigen KW-8; E3 ubiquitin protein ligase CHIP; E3 ubiquitin-protein ligase CHIP; Heat shock protein A binding protein 2 (c terminal); HSPABP2; NY CO 7; PP1131; SDCCAG7; Serologically defined colon cancer antigen 7; STIP1 homology and U Box containing protein 1; STIP1 homology and U box containing protein 1 E3 ubiquitin protein ligase; STIP1 homology and U box-containing protein 1; STUB 1; STUB1; UBOX 1; UBOX1
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-303
  • 氨基酸序列
    MKGKEEKEGG ARLGAGGGSP EKSPSAQELK EQGNRLFVGR KYPEAAACYG RAITRNPLVA VYYTNRALCY LKMQQHEQAL ADCRRALELD GQSVKAHFFL GQCQLEMESY DEAIANLQRA YSLAKEQRLN FGDDIPSALR IAKKKRWNSI EERRIHQESE LHSYLSRLIA AERERELEEC QRNHEGDEDD SHVRAQQACI EAKHDKYMAD MDELFSQVDE KRKKRDIPDY LCGKISFELM REPCITPSGI TYDRKDIEEH LQRVGHFDPV TRSPLTQEQL IPNLAMKEVI DAFISENGWV EDY
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    E3 ubiquitin-protein ligase which targets misfolded chaperone substrates towards proteasomal degradation. Collaborates with ATXN3 in the degradation of misfolded chaperone substrates: ATXN3 restricting the length of ubiquitin chain attached to STUB1/CHIP substrates and preventing further chain extension. Ubiquitinates NOS1 in concert with Hsp70 and Hsp40. Modulates the activity of several chaperone complexes, including Hsp70, Hsc70 and Hsp90. Mediates transfer of non-canonical short ubiquitin chains to HSPA8 that have no effect on HSPA8 degradation. Mediates polyubiquitination of DNA polymerase beta (POLB) at 'Lys-41', 'Lys-61' and 'Lys-81', thereby playing a role in base-excision repair: catalyzes polyubiquitination by amplifying the HUWE1/ARF-BP1-dependent monoubiquitination and leading to POLB-degradation by the proteasome. Mediates polyubiquitination of CYP3A4. Ubiquitinates EPHA2 and may regulate the receptor stability and activity through proteasomal degradation. Acts as a co-chaperone for HSPA1A and HSPA1B chaperone proteins and promotes ubiquitin-mediated protein degradation. Negatively regulates the suppressive function of regulatory T-cells (Treg) during inflammation by mediating the ubiquitination and degradation of FOXP3 in a HSPA1A/B-dependent manner. Likely mediates polyubiquitination and downregulates plasma membrane expression of PD-L1/CD274, an immune inhibitory ligand critical for immune tolerance to self and antitumor immunity. Negatively regulates TGF-beta signaling by modulating the basal level of SMAD3 via ubiquitin-mediated degradation. May regulate myosin assembly in striated muscles together with UBE4B and VCP/p97 by targeting myosin chaperone UNC45B for proteasomal degradation. Mediates ubiquitination of RIPK3 leading to its subsequent proteasome-dependent degradation.
  • 基因功能参考文献：
    1. Data suggest that the molecular basis of genetic defects in CHIP that cause SCAR16 is that most mutations observed in patients with SCAR16 result in destabilization of CHIP leading to down-regulation of it's biquitination function. (SCAR16 = spinocerebellar ataxia autosomal recessive type 16) PMID: 29317501
    2. these findings suggest that CHIP plays a role in negative regulation of PINK1 stability and may suppress PINK1's cytoprotective effect during staurosporine-induced mammalian cell death. PMID: 29242192
    3. Results identified a new role of CHIP in adipocyte differentiation. CHIP interacts with and mediates the ubiquitylation of PPARgamma , which results in negative effects on adipogenesis. PMID: 28059128
    4. The overexpression of CHIP significantly increased the migration and invasion of the DU145 cells, which is possible due to activation of the AKT signaling pathway and upregulation of vimentin. The expression level of CHIP was observed to be increased in human prostate cancer tissues compared with the adjacent normal tissue. PMID: 29693147
    5. Here the s show that the mammalian ubiquitin ligase C-terminal Hsp70-interacting protein (CHIP), if freed from chaperones during acute stress, can dock on cellular membranes thus performing a proteostasis sensor function. PMID: 29091030
    6. These results suggest that in the early response to stressful stimuli, MLK4beta-MLK3 binding is important for regulating MLK3 activity and MAPK signalling, and after prolonged periods of stress exposure, MLK4beta and MLK3 proteins decline via CHIP-dependent degradation. PMID: 28757353
    7. Prostate cancer cells expressing an S273A mutant of CHIP have attenuated AR degradation upon 2-ME treatment compared with cells expressing wild-type CHIP, supporting the idea that CHIP phosphorylation by Aurora A activates its E3 ligase activity for the AR PMID: 28536143
    8. CHIP role in lung cancer radioresistance.p21 is a bona fide ubiquitylation substrate for CHIP. PMID: 28232384
    9. PC-1 works in conjunction with E3 ligase CHIP to regulate androgen receptor stability and activity. PMID: 27835608
    10. Some STUB1 mutations known to cause spinocerebellar ataxia, autosomal recessive 16 have a profound impact on the protein structure, stability, and ability of CHIP to dimerize in vitro. PMID: 28396517
    11. These findings provide clinical and imaging support for the notion that CHIP is a crucial converging point of manifold neurodegenerative processes, corresponding with its universal biological function in neurodegeneration and reveal the second STUB1 family with ataxia plus hypogonadism. PMID: 28193273
    12. the CHIP/CLEC-2 axis may play an important role in the modulation of immune response. PMID: 27443248
    13. The overexpression of CHIP is a potent prognostic factor of a good prognosis in ER-positive breast cancer patients in the postmenopausal phase. PMID: 27334118
    14. these findings indicate that the stability of the DDIAS protein is regulated by CHIP/HSP70-mediated proteasomal degradation and that CHIP overexpression stimulates the apoptosis of lung cancer cells in response to DNA-damaging agents PMID: 28079882
    15. Study reveals a mechanism that the Warburg effect is regulated by CHIP through its function as an E3 ligase, which mediates the degradation of PKM2 during tumor progression. PMID: 28346425
    16. The E3 ubiquitin ligase STUB1 is a negative regulator of both RUNX1 and RUNX1-RUNX1T1. Activation of STUB1 could be a promising therapeutic strategy for RUNX1-RUNX1T1 leukemia. PMID: 28536267
    17. Low CHIP expression is associated with metastasis of glioblastoma. PMID: 27546621
    18. Data show that carboxyl-terminus of Hsp70-interacting protein (CHIP) promotes polyubiquitination of transglutaminase 2 (TG2) and its subsequent proteasomal degradation. PMID: 26568304
    19. Consistent with reduced transcription factor EB (TFEB) activity, accumulation of phosphorylated TFEB in STUB1-deficient cells resulted in reduced autophagy and reduced mitochondrial biogenesis. These studies reveal that the ubiquitin-proteasome pathway participates in regulating autophagy and lysosomal functions by regulating the activity of TFEB. PMID: 28754656
    20. we report the identification of an unconventional p14ARF degradation pathway induced by the chaperone HSP90 in association with the E3 ubiquitin ligase C-terminus of HSP70-interacting protein (CHIP). PMID: 27793846
    21. C terminus of Hsc70-interacting protein (CHIP) selectively interacted with epidermal growth factor receptor (EGFR) mutants and simultaneously induced their ubiquitination and proteasomal degradation. PMID: 27475501
    22. Study reveals an important function of CHIP-mediated proteolysis in insulin and IGF1 signaling; upon proteotoxic stress conditions and during aging, CHIP is recruited toward disposal of misfolded proteins, reducing its capacity to degrade the INSR; identify a degradation pathway that controls the level of active DAF-2/INSR in C. elegans, Drosophila and human cells. PMID: 28431247
    23. Overexpression of CHIP decreased intracellular protein levels of both G2385R mutant and wild-type LRRK2, while short interfering RNA CHIP knockdown had the opposite effect PMID: 28320779
    24. CHIP directly regulates the stability of CD166 protein through the ubiquitin proteasome system. PMID: 28279658
    25. Data show that BAG2 Inhibits CHIP-Mediated HSP72 ubiquitination in aged cells. PMID: 28042827
    26. Data show that transcription factor regulatory factor X 1 (RFX1) protein expression can be tightly regulated by polyubiquitination-mediated proteosomal degradation via STIP1 homology and U-box containing protein 1 (STUB1). PMID: 27283392
    27. CHIP may serve as a promising prognostic biomarker for non-small cell lung cancer (NSCLC] patients and it may be involved in NSCLC angiogenesis through regulating VEGF secretion and expression of VEGFR2. PMID: 27392029
    28. Cdk5-mediated phosphorylation of CHIP negatively regulates its neuroprotective function, thereby contributing to neuronal cell death progression following neurotoxic stimuli. PMID: 26206088
    29. CHIP is a bona fide negative regulator of the RIPK1-RIPK3 necrosome formation leading to desensitization of TNF-mediated necroptosis PMID: 26900751
    30. Protein-protein interactions modulate the docking-dependent E3-ubiquitin ligase activity of CHIP. PMID: 26330542
    31. CHIP is required for protein quality control (PQC) and CHIP knockdown diminished cellular PQC capacity in lens cells. PMID: 26321754
    32. Data show that the E3 ubiquitin ligase CHIP interacts with protein arginine methyltransferase-5 (PRMT5) both in vivo and in vitro. PMID: 26658161
    33. These results indicate that CHIP decreases the Kv1.5 protein level and functional channel by facilitating its degradation in concert with chaperone Hsc70 PMID: 26232501
    34. Data suggest that CHIP plays roles in the regulation of autophagic flux. PMID: 26219223
    35. detailed and systematic investigation to characterize if there are significant differences in the CHIP in vitro ubiquitination of human Hsp70 and Hsc70. PMID: 26010904
    36. CHIP stabilizes amyloid precursor protein via proteasomal degradation and p53-mediated trans-repression of BACE1. PMID: 25773675
    37. Our findings demonstrate for the first time that CHIP may be involved in RCC angiogenesis through regulating VEGF secretion and expression of VEGFR2. PMID: 26021863
    38. High expression of CHIP is associated with HBV-related Hepatocellular Carcinoma. PMID: 25987026
    39. results indicate that the post-endocytic ubiquitination of CFTR by CHIP is a critical step in the peripheral quality control of cell surface DeltaF508 CFTR PMID: 25879443
    40. these observations provide functional evidence for CHIP behaviors as a tumor suppressor in gastric cancer. PMID: 25672477
    41. CHIP docks onto Hsp70/Hsc70 and defines a bipartite mode of interaction between TPR domains and their binding partners. PMID: 25684577
    42. CHIP masks genetic variations to suppress heterogeneous Bcl-2 expression levels and prevents augmentation of the anticancer drug-resistant population of breast cancer cells. PMID: 25435366
    43. miR-1178 acts as an oncomiR in pancreatic cancer cells by inhibiting CHIP expression. PMID: 25635996
    44. CHIP/TRAF3/NIK interactions recruit NIK to E3 ligase complexes for ubiquitination and degradation, thus maintaining NIK at low levels PMID: 25792747
    45. Whether the clinical heterogeneity seen in STUB1 ARCA can be related to the location of the mutations remains to be understood PMID: 25258038
    46. These observations indicate that CHIP serves as a novel tumor suppressor by downregulating EGFR pathway in pancreatic cancer cells, decreased expression of CHIP was associated with poor prognosis in pancreatic cancer. PMID: 24722501
    47. CHIP-mediated ubiquitination of IRE1 contributes to the dynamic regulation of the unfolded protein response. PMID: 25225294
    48. From our results, we propose that inhibition of CSC properties may be one of the functions of CHIP as a suppressor of cancer progression. PMID: 25234599
    49. CHIP interacted with eIF5A and mediated its ubiquitination for degradation. PMID: 24509416
    50. Ebp1 p42 isoform regulates the proteasomal degradation of the p85 regulatory subunit of PI3K by recruiting a chaperone-E3 ligase complex HSP70/CHIP. PMID: 24651434

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  • 相关疾病：
    Spinocerebellar ataxia, autosomal recessive, 16 (SCAR16)
  • 亚细胞定位：
    Cytoplasm. Nucleus.
  • 组织特异性：
    Expressed in differentiated myotubes (at protein level). Highly expressed in skeletal muscle, heart, pancreas, brain and placenta. Detected in kidney, liver and lung.
  • 数据库链接：

    HGNC: 11427

    OMIM: 607207

    KEGG: hsa:10273

    STRING: 9606.ENSP00000219548

    UniGene: Hs.592081