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  4. Recombinant Human E3 ubiquitin-protein ligase LNX (LNX1)

Recombinant Human E3 ubiquitin-protein ligase LNX (LNX1)

  • 中文名称：
    人LNX1重组蛋白
  • 货号：
    CSB-YP848393HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人LNX1重组蛋白
  • 货号：
    CSB-EP848393HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人LNX1重组蛋白
  • 货号：
    CSB-EP848393HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人LNX1重组蛋白
  • 货号：
    CSB-BP848393HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人LNX1重组蛋白
  • 货号：
    CSB-MP848393HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    LNX1
  • Uniprot No.：
  • 别名：
    LNX1; LNX; PDZRN2; UNQ574/PRO1136; E3 ubiquitin-protein ligase LNX; EC 2.3.2.27; Ligand of Numb-protein X 1; Numb-binding protein 1; PDZ domain-containing RING finger protein 2; RING-type E3 ubiquitin transferase LNX
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-728
  • 氨基酸序列
    MNQPESANDP EPLCAVCGQA HSLEENHFYS YPEEVDDDLI CHICLQALLD PLDTPCGHTY CTLCLTNFLV EKDFCPMDRK PLVLQHCKKS SILVNKLLNK LLVTCPFREH CTQVLQRCDL EHHFQTSCKG ASHYGLTKDR KRRSQDGCPD GCASLTATAP SPEVSAAATI SLMTDEPGLD NPAYVSSAED GQPAISPVDS GRSNRTRARP FERSTIRSRS FKKINRALSV LRRTKSGSAV ANHADQGREN SENTTAPEVF PRLYHLIPDG EITSIKINRV DPSESLSIRL VGGSETPLVH IIIQHIYRDG VIARDGRLLP GDIILKVNGM DISNVPHNYA VRLLRQPCQV LWLTVMREQK FRSRNNGQAP DAYRPRDDSF HVILNKSSPE EQLGIKLVRK VDEPGVFIFN VLDGGVAYRH GQLEENDRVL AINGHDLRYG SPESAAHLIQ ASERRVHLVV SRQVRQRSPD IFQEAGWNSN GSWSPGPGER SNTPKPLHPT ITCHEKVVNI QKDPGESLGM TVAGGASHRE WDLPIYVISV EPGGVISRDG RIKTGDILLN VDGVELTEVS RSEAVALLKR TSSSIVLKAL EVKEYEPQED CSSPAALDSN HNMAPPSDWS PSWVMWLELP RCLYNCKDIV LRRNTAGSLG FCIVGGYEEY NGNKPFFIKS IVEGTPAYND GRIRCGDILL AVNGRSTSGM IHACLARLLK ELKGRITLTI VSWPGTFL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of NUMB. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates ubiquitination of isoform p66 and isoform p72 of NUMB, but not that of isoform p71 or isoform p65.; Isoform 2 provides an endocytic scaffold for IGSF5/JAM4.
  • 基因功能参考文献：
    1. study provided the evidences that LNX1 signaling plays important roles in regulating the stemness of colon cancer cells PMID: 29190716
    2. LNX was closely related to human gliomas and suggested it plays an important role in gliomas by notch signaling. PMID: 16324299
    3. LNX1-mediated ubiquitination and degradation of PBK inhibited cell proliferation and enhanced sensitivity to doxorubicin-induced apoptosis. PMID: 22889411
    4. identify and confirm six novel LNX1 binding partners: KCNA4, PAK6, PLEKHG5, PKC-alpha1, TYK2 and PBK, and suggest that LNX1 functions as a signalling scaffold. PMID: 22087225
    5. The s found a high level of LNX1 and LNX2 mRNAs in endocarditis, the principal manifestation of chronic Q fever, but not in acute Q fever. PMID: 22066909
    6. LNX1 and LNX2 interact with CD8alpha and promote its ubiquitylation and endocytosis PMID: 22045731
    7. Down-regulation of LNX could result in cell cycle arrest in G0/G1 phase through inhibition of beta-catenin, MAPK, NFkappaB, c-Myc-dependent pathway and activation of p53, TGF-beta-dependent pathway. PMID: 21104141
    8. LNX and RhoC might be part of a larger protein complex that would have important functions in signaling transduction about regulating the transcriptional activities of AP-1. PMID: 19701800
    9. identifed ligand of Numb protein X as an Np9-interacting partner; findings point to the possibility that Np9 affects tumorigenesis through the LNX/Numb/Notch pathway PMID: 15367597
    10. The presence of multiple protein binding domains involved in signal transduction and interaction with Numb and SKIP suggested an important role for LNX in tumorogenesis. PMID: 16002321
    11. The interaction of c-Src with LNX1 depends on the C-terminal PDZ ligand of c-Src. PMID: 17936276
    12. Both gene sequence alterations and amplifications of LNX1 and Numbl are present in a subset of human gliomas. PMID: 18940473
    13. A direct interaction between ErbB2 and mouse LNX1 was identified, suggesting that LNX1 may be involved in neuregulin-1/ErbB signaling in perisynaptic Schwann cells. PMID: 16122940
    14. The tyrosine phosphorylation of the LDNPAY sequence motif in mouse LNX could generate a binding site for the phosphorylation-dependent binding of other PTB domain-containing proteins. PMID: 9535908
    15. Describes various glioblastoma multiforms containing the amplicon on chromosomal segment 4q12, including the LNX1 gene. PMID: 17504929

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  • 亚细胞定位：
    Cytoplasm.
  • 组织特异性：
    Expressed in heart, placenta, kidney, pancreas and brain.
  • 数据库链接：

    HGNC: 6657

    OMIM: 609732

    KEGG: hsa:84708

    STRING: 9606.ENSP00000263925

    UniGene: Hs.518760