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  4. Recombinant Human ELL-associated factor 2 (EAF2)

Recombinant Human ELL-associated factor 2 (EAF2)

  • 中文名称：
    人EAF2重组蛋白
  • 货号：
    CSB-YP856910HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人EAF2重组蛋白
  • 货号：
    CSB-EP856910HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人EAF2重组蛋白
  • 货号：
    CSB-EP856910HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人EAF2重组蛋白
  • 货号：
    CSB-BP856910HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人EAF2重组蛋白
  • 货号：
    CSB-MP856910HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    EAF2
  • Uniprot No.：
  • 别名：
    BM 040; BM040; EAF 2; EAF2; EAF2_HUMAN; Ehrlich S II transcriptional activator factor ; ELL associated factor 2; ELL-associated factor 2; Festa; Testosterone regulated apoptosis inducer and tumor suppressor; Testosterone regulated apoptosis inducer and tumor suppressor protein; Testosterone-regulated apoptosis inducer and tumor suppressor protein; TRAITS; U19; Uncharacterized bone marrow protein BM 040; Uncharacterized bone marrow protein BM040
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-260
  • 氨基酸序列
    MNSAAGFSHL DRRERVLKLG ESFEKQPRCA FHTVRYDFKP ASIDTSSEGY LEVGEGEQVT ITLPNIEGST PPVTVFKGSK KPYLKECILI INHDTGECRL EKLSSNITVK KTRVEGSSKI QYRKEQQQQQ MWNSARTPNL VKHSPSEDKM SPASPIDDIE RELKAEASLM DQMSSCDSSS DSKSSSSSSS EDSSSDSEDE DCKSSTSDTG NCVSGHPTMT QYRIPDIDAS HNRFRDNSGL LMNTLRNDLQ LSESGSDSDD
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Acts as a transcriptional transactivator of TCEA1 elongation activity. Acts as a transcriptional transactivator of ELL and ELL2 elongation activities. Potent inducer of apoptosis in prostatic and non-prostatic cell lines. Inhibits prostate tumor growth in vivo.
  • 基因功能参考文献：
    1. Simultaneous inactivation of EAF2 and p53 can act to activate STAT3 and drive prostate tumorigenesis. PMID: 29518696
    2. The results indicated that Eaf2 was able to effectively suppress H2O2induced apoptosis of HLE cells via inhibition of caspase 3 production and activation of Wnt3a signaling. PMID: 29257273
    3. EZH2 promotes tumorigenesis and malignant progression in part by activating glycolysis through an EAF2-HIF1alpha signaling axis. PMID: 27259264
    4. Concurrent p53 nuclear staining and EAF2 downregulation was associated with high Gleason score In human prostate cancer specimens. PMID: 29029019
    5. analysis showed that EAF2 and EAF1 are required for the recruitment and retention of Ku70/Ku80 to DNA damage sites and play a functional role in nonhomologous end-joining DNA repair. Findings provide evidence for EAF2 as a key factor mediating androgen protection of DNA damage via Ku70/Ku80 in prostate cancer cells. PMID: 27721405
    6. EAF2 binding does not alter Smad3 phosphorylation but causes Smad3 cytoplasmic retention, competes with Smad4 for binding to Smad3, and prevents p300-Smad3 complex formation PMID: 26370086
    7. Findings suggest that regulation of the AR signaling pathway, cell proliferation, and migration through FOXA1 represents an important mechanism of EAF2 suppression of prostate carcinogenesis. PMID: 25808853
    8. Together these findings identify a novel physical and functional interaction between EAF2 and the Rb pathway. PMID: 24727455
    9. EAF2 bound to and suppressed HIF-1alpha. PMID: 24421387
    10. the role of the EAF2 in response to simvastatin and lovastatin in HCT-116 colon cancer cells PMID: 21483694
    11. Data suggest that U19/EAF2 regulates the expression of TSP-1 via blocking p53 repression of the TSP-1 promoter. PMID: 19826414
    12. U19 is growth inhibitory and tumor suppressive and that the disruption of androgen-dependent growth inhibition via U19 down-regulation is commonly associated with prostate cancer progression. PMID: 12907652
    13. ELL-associated factors 1 and 2 are positive regulators of RNA polymerase II elongation factor ELL. PMID: 16006523
    14. ELL may be an important factor required for U19/Eaf2 function because U19/Eaf2 nuclear localization and transactivation activity are essential for its function as a transcription factor. PMID: 16114057
    15. FB1 is an important binding partner and a functional regulator of U19/EAF2, EAF1, and/or ELL. PMID: 17395368

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  • 亚细胞定位：
    Nucleus speckle.
  • 蛋白家族：
    EAF family
  • 组织特异性：
    Expressed in heart, brain, placenta, lung, skeletal muscle, kidney, pancreas, spleen, prostate, testis, small intestine, colon, adrenal, bone marrow, lymph node, spinal gland, stomach, thyroid, trachea, thymus, liver and leukocytes.
  • 数据库链接：

    HGNC: 23115

    OMIM: 607659

    KEGG: hsa:55840

    STRING: 9606.ENSP00000273668

    UniGene: Hs.477325