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  4. Recombinant Human FAS-associated factor 1 (FAF1)

Recombinant Human FAS-associated factor 1 (FAF1)

  • 中文名称：
    人FAF1重组蛋白
  • 货号：
    CSB-YP892359HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人FAF1重组蛋白
  • 货号：
    CSB-EP892359HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人FAF1重组蛋白
  • 货号：
    CSB-EP892359HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人FAF1重组蛋白
  • 货号：
    CSB-BP892359HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人FAF1重组蛋白
  • 货号：
    CSB-MP892359HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    CGI 03; FAF1; FAF1 protein; FAF1_HUMAN; Fas (TNFRSF6) associated factor 1; FAS associated factor 1; Fas associated protein factor 1; FAS-associated factor 1; FLJ37524; hFAF1; HFAF1s; Protein FAF1; TNFRSF6 associated factor 1; UBX domain protein 3A; UBX domain-containing protein 12; UBX domain-containing protein 3A; UBXD12; UBXN3A
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-650
  • 氨基酸序列
    MASNMDREMI LADFQACTGI ENIDEAITLL EQNNWDLVAA INGVIPQENG ILQSEYGGET IPGPAFNPAS HPASAPTSSS SSAFRPVMPS RQIVERQPRM LDFRVEYRDR NVDVVLEDTC TVGEIKQILE NELQIPVSKM LLKGWKTGDV EDSTVLKSLH LPKNNSLYVL TPDLPPPSSS SHAGALQESL NQNFMLIITH REVQREYNLN FSGSSTIQEV KRNVYDLTSI PVRHQLWEGW PTSATDDSMC LAESGLSYPC HRLTVGRRSS PAQTREQSEE QITDVHMVSD SDGDDFEDAT EFGVDDGEVF GMASSALRKS PMMPENAENE GDALLQFTAE FSSRYGDCHP VFFIGSLEAA FQEAFYVKAR DRKLLAIYLH HDESVLTNVF CSQMLCAESI VSYLSQNFIT WAWDLTKDSN RARFLTMCNR HFGSVVAQTI RTQKTDQFPL FLIIMGKRSS NEVLNVIQGN TTVDELMMRL MAAMEIFTAQ QQEDIKDEDE REARENVKRE QDEAYRLSLE ADRAKREAHE REMAEQFRLE QIRKEQEEER EAIRLSLEQA LPPEPKEENA EPVSKLRIRT PSGEFLERRF LASNKLQIVF DFVASKGFPW DEYKLLSTFP RRDVTQLDPN KSLLEVKLFP QETLFLEAKE
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Ubiquitin-binding protein. Required for the progression of DNA replication forks by targeting DNA replication licensing factor CDT1 for degradation. Potentiates but cannot initiate FAS-induced apoptosis.
  • 基因功能参考文献：
    1. Data show that activated proto-oncogene protein Akt (AKT) directly phosphorylates Fas associated factor 1 (FAF1) reduces FAF1 at the plasma membrane and results in an increase in TGF-beta type II receptor (TbetaRII) at the cell surface. PMID: 28443643
    2. FAF1 was vital in the proliferation of NP cells by modulating the Erk signaling pathway, which suggests that FAF1 may be a novel marker in the early diagnosis of Intervertebral disc degeneration. PMID: 29257270
    3. Helicobacter pylori, a risk factor for gastric cancer, down-regulated FAF1 expression via NF-kappaB signaling in gastric carcinoma cells, promoting tumor growth. PMID: 28030825
    4. Knockdown of FAF1 suppressed Sanguinarine (SNG)-induced inhibition of cell proliferation, invasion, and migration and induction of cell cycle arrest and apoptosis in NSCLC cells. SNG also inhibited implanted tumor growth and increased FAF1 expression in tumors in vivo. PMID: 28296008
    5. These data demonstrate the presence of an FAF1-PARP1 axis that is involved in oxidative stress-induced necrosis and in the pathology of Parkinson's disease. PMID: 27662363
    6. FAF1 physically interacts with XIAP. FAF1 attenuates XIAP-mediated NF-kappaB activation. PMID: 28414080
    7. confirmation of the association between rs17106184 (FAF1) and Type 2 Diabetes in a northern Han Chinese population PMID: 27589775
    8. FAF1 plays a novel role in negatively regulating virus-induced IFN-beta production and the antiviral response by inhibiting the translocation of active, phosphorylated IRF3 from the cytosol to the nucleus PMID: 26811330
    9. Progression of the DNA replication fork is coordinated by FAF1. FAF1 binds to the licensing factor CDT-1 and additional ubiquitylated proteins, thus promoting CDC48-dependent turnover and disassembly of DNA replication factor complexes. PMID: 26842564
    10. Report a novel role for FAF1 in regulating CD40-induced NFkappaB activation via a negative feedback loop. PMID: 24810049
    11. The repertoire of VAPB interactors is more diverse than previously anticipated and link VAPB to the function of ATPase complexes such as p97/FAF1 and ASNA1/transmembrane-domain recognition complex. PMID: 24885147
    12. long-chain unsaturated fatty acids also induced polymerization of Fas-associated factor 1 (FAF1), the only other mammalian protein that contains a UAS domain homologous to that of Ubxd8 PMID: 23720822
    13. Data indicate that Npl4-Ufd1 heterodimer is required for VCP-FAF1 interaction. PMID: 23293021
    14. hFAF1 inhibits tumor formation by increasing the degradation of Hsp70 mediated via its UBA domain. PMID: 22876279
    15. Fas-associated factor 1 is a scaffold protein that promotes beta-transducin repeat-containing protein (beta-TrCP)-mediated beta-catenin ubiquitination and degradation. PMID: 22730322
    16. Wild-type p97/VCP ND1 in complex with FAF1 UBX crystallized into very thin sheet-shaped crystals which turned out to be of poor diffraction quality PMID: 22102026
    17. The s report the first in vitro confirmed non-homotypic interaction between NLRP12 pyrin domain and the pro-apoptotic protein Fas-associated factor 1 (FAF-1), which links the innate immune system to apoptotic signaling. PMID: 21978668
    18. In transgenic mice in which FAF1 is expressed in immature olfactory sensory neurons (OSNs), FAF1 not only misroutes OSN axons to deep layers of the olfactory bulb but also disrupts the glomerular layer PMID: 21849551
    19. FAF1 and UBXD7 only bind to p97-UFD1/NPL4, but not free p97, thus demonstrating for the first time a hierarchy in p97-cofactor interactions PMID: 21645854
    20. the crystal structure of human FAF1 UBX domain at 2.9A resolution is reported. PMID: 21414298
    21. Mutations in FAF1 is associated with cleft palate and is necessary for lower jaw development in zebrafish. PMID: 21295280
    22. Data show that miR-24 regulates apoptosis by targeting FAF1 in cancer cells. PMID: 20195546
    23. the p97/VCP N domain was crystallized in complex with the FAF1 UBX domain. X-ray data were collected to 2.60 A resolution and the crystals belonged to space group C222(1), with unit-cell parameters a = 58.24, b = 72.81, c = 132.93 A. PMID: 20057067
    24. report FAF1 UBX domain crystallization and X-ray data collection. X-ray data were collected to 3.00 A resolution and the crystals belonged to space group F4(1)32, with unit-cell parameters a = b = c = 176.40 A. PMID: 20124726
    25. Determined the crystal structure of the ubiquitin-associated domain of human FAF1 (hFAF1-UBA) and examined its interaction with ubiquitin and ubiquitin-like proteins using nuclear magnetic resonance. PMID: 19722279
    26. FAF1 is a member of Fas-DISC acting upstream of caspase-8 PMID: 12702723
    27. phosphorylation by protein kinase CK2 influencing transport into nucleus PMID: 12832043
    28. FAS-associated factor 1 is specifically reduced in human gastric carcinomas PMID: 12963981
    29. a novel function of FAF1: NF-kappaB activity suppressor by cytoplasmic retention of NF-kappaB p65 via physical interaction. PMID: 14600157
    30. hFAF1 prevents cells from recovery after stress by binding to and inhibiting the chaperone activity of Hsp70 PMID: 15596450
    31. hFAF1 may serve as a scaffolding protein that regulates protein degradation in the ubiquitin-proteasome pathway. PMID: 15743842
    32. FAF1 functions as a negative regulator of an NF-kappaB signal pathway that involves PYPAF1 and ASC. PMID: 17046979
    33. FAF1 robustly suppresses NF-kappaB activation through the inhibition of IKK activation in combination with previously reported cytoplasmic retention of NF-kappaB p65 PMID: 17684021
    34. Fas expression was reduced and caspase-10 activity was decreased in both patients PMID: 17999750
    35. Changes in FAF1 expression were specific to PD-related alpha-synuclein pathology and nigral cell loss. These studies, together with previous genetic linkage studies, highlight the potential significance of FAF1 in pathogenesis of idiopathic PD. PMID: 18573343
    36. analysis of the negative feedback regulation of Aurora-A via phosphorylation of Fas-associated factor-1 PMID: 18790738

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  • 亚细胞定位：
    Nucleus.
  • 组织特异性：
    Most abundant in testis, slightly less abundant in skeletal muscle and heart, followed by prostate, thymus, ovary, small intestine, and colon. Not detected in the peripheral blood leukocytes.
  • 数据库链接：

    HGNC: 3578

    OMIM: 604460

    KEGG: hsa:11124

    STRING: 9606.ENSP00000360843

    UniGene: Hs.530402