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  4. Recombinant Human Hemogen (HEMGN)

Recombinant Human Hemogen (HEMGN)

  • 货号：
    CSB-YP010284HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP010284HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP010284HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP010284HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP010284HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    HEMGN
  • Uniprot No.：
  • 别名：
    CT155; EDAG; EDAG-1; Erythroid differentiation-associated gene protein; Hemgn; HEMGN_HUMAN; Hemogen; Hemopoietic gene protein; NDR; Negative differentiation regulator protein
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-484
  • 氨基酸序列
    MDLGKDQSHL KHHQTPDPHQ EENHSPEVIG TWSLRNRELL RKRKAEVHEK ETSQWLFGEQ KKRKQQRTGK GNRRGRKRQQ NTELKVEPQP QIEKEIVEKA LAPIEKKTEP PGSITKVFPS VASPQKVVPE EHFSEICQES NIYQENFSEY QEIAVQNHSS ETCQHVSEPE DLSPKMYQEI SVLQDNSSKI CQDMKEPEDN SPNTCQVISV IQDHPFKMYQ DMAKREDLAP KMCQEAAVPK ILPCPTSEDT ADLAGCSLQA YPKPDVPKGY ILDTDQNPAE PEEYNETDQG IAETEGLFPK IQEIAEPKDL STKTHQESAE PKYLPHKTCN EIIVPKAPSH KTIQETPHSE DYSIEINQET PGSEKYSPET YQEIPGLEEY SPEIYQETSQ LEEYSPEIYQ ETPGPEDLST ETYKNKDVPK ECFPEPHQET GGPQGQDPKA HQEDAKDAYT FPQEMKEKPK EEPGIPAILN ESHPENDVYS YVLF
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Regulates the proliferation and differentiation of hematopoietic cells. Overexpression block the TPA-induced megakaryocytic differentiation in the K562 cell model. May also prevent cell apoptosis through the activation of the nuclear factor-kappa B (NF-kB).
  • 基因功能参考文献：
    1. Using ex vivo culture and HSC transplantation models, study finds that EDAG enhances proliferative potential of human cord blood CD34+ cells, increases survival, prevents cell apoptosis and promotes their repopulating capacity. EDAG overexpression induces rapid entry of CD34+ cells into the cell cycle. Gene expression profile analysis shows that EDAG knockdown leads to down-regulation of positive cell cycle regulators. PMID: 29324880
    2. a 7-gene signature was identified which correctly predicted the primary prefibrotic myelofibrosis group with a sensitivity of 100% and a specificity of 89%. The 7 genes included MPO, CEACAM8, CRISP3, MS4A3, CEACAM6, HEMGN, and MMP8 PMID: 27579896
    3. EDAG-1 regulates the proliferation and apoptosis of thyroid carcinoma via the PI3K/Akt signaling pathway. PMID: 27261581
    4. EDAG forms complex with GATA1 and p300 and increases GATA1 acetylation and transcriptional activity by facilitating the interaction between GATA1 and p300 PMID: 24740910
    5. EDAG enhances the protein stability of NPM1 via binding to NPM1, which plays a critical role in the anti-apoptosis of leukaemia cells. PMID: 22712502
    6. EDAG functions as a positive regulator of erythroid/megakaryocytic differentiation in 32D cells associated with the induction of GATA-1 and its target genes PMID: 20564185
    7. results suggest a novel function of nuclear negative differentiation regulator (NDR) protein in regulating hematopoietic cell development [NDR] PMID: 14730214
    8. These results suggest that EDAG regulates the proliferation and differentiation of hematopoietic cells and resists cell apoptosis through the activation of NF-kappa B. PMID: 15332117
    9. Hemgn promoter contains critical regulatory elements for its transcription in hematopoietic tissues and Hemgn is a direct target of GATA1 in leukemia cells PMID: 16437149
    10. The stable transfected cells were identified with RT-PCR and the effect of EDAG/siRNA on the growth of the human erythroleukemia cell line HEL was analyzed. PMID: 17671716
    11. The inhibition of the EDAG gene by PMA is mediated through down-regulation of transcription factor GATA-1 . PMID: 18599389

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  • 亚细胞定位：
    Nucleus.
  • 组织特异性：
    Expressed in hematopoietic precursor cells, thyroid and spermatids (at protein level). Expressed in bone marrow, testis, thymus. Expressed in prostate cancer and ovarian cancer. Also expressed in thymus and thyroid tumors, non-Hodgkin lymphoma, various le
  • 数据库链接：

    HGNC: 17509

    OMIM: 610715

    KEGG: hsa:55363

    STRING: 9606.ENSP00000259456

    UniGene: Hs.176626