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  4. Recombinant Human Histone-lysine N-methyltransferase SUV420H2 (SUV420H2)

Recombinant Human Histone-lysine N-methyltransferase SUV420H2 (SUV420H2)

  • 中文名称：
    人KMT5C重组蛋白
  • 货号：
    CSB-YP769808HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人KMT5C重组蛋白
  • 货号：
    CSB-EP769808HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人KMT5C重组蛋白
  • 货号：
    CSB-EP769808HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人KMT5C重组蛋白
  • 货号：
    CSB-BP769808HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人KMT5C重组蛋白
  • 货号：
    CSB-MP769808HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    KMT5C
  • Uniprot No.：
  • 别名：
    FLJ98627; Histone lysine N methyltransferase SUV420H2; Histone-lysine N-methyltransferase SUV420H2; KMT5C; Lysine N methyltransferase 5C; Lysine N-methyltransferase 5C; MGC2705; Su(var)4 20 homolog 2; Su(var)4-20 homolog 2; Suppressor of variegation 4 20 homolog 2; Suppressor of variegation 4-20 homolog 2; Suv4 20h2; Suv4-20h2; SUV420H2; SV422_HUMAN
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-462
  • 氨基酸序列
    MGPDRVTARE LCENDDLATS LVLDPYLGFR THKMNVSPVP PLRRQQHLRS ALETFLRQRD LEAAYRALTL GGWTARYFQS RGPRQEAALK THVYRYLRAF LPESGFTILP CTRYSMETNG AKIVSTRAWK KNEKLELLVG CIAELREADE GLLRAGENDF SIMYSTRKRS AQLWLGPAAF INHDCKPNCK FVPADGNAAC VKVLRDIEPG DEVTCFYGEG FFGEKNEHCE CHTCERKGEG AFRTRPREPA LPPRPLDKYQ LRETKRRLQQ GLDSGSRQGL LGPRACVHPS PLRRDPFCAA CQPLRLPACS ARPDTSPLWL QWLPQPQPRV RPRKRRRPRP RRAPVLSTHH AARVSLHRWG GCGPHCRLRG EALVALGQPP HARWAPQQDW HWARRYGLPY VVRVDLRRLA PAPPATPAPA GTPGPILIPK QALAFAPFSP PKRLRLVVSH GSIDLDVGGE EL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Histone methyltransferase that specifically methylates monomethylated 'Lys-20' (H4K20me1) and dimethylated 'Lys-20' (H4K20me2) of histone H4 to produce respectively dimethylated 'Lys-20' (H4K20me2) and trimethylated 'Lys-20' (H4K20me3) and thus regulates transcription and maintenance of genome integrity. In vitro also methylates unmodified 'Lys-20' (H4K20me0) of histone H4 and nucleosomes. H4 'Lys-20' trimethylation represents a specific tag for epigenetic transcriptional repression. Mainly functions in pericentric heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin in these regions. KMT5C is targeted to histone H3 via its interaction with RB1 family proteins (RB1, RBL1 and RBL2). Facilitates TP53BP1 foci formation upon DNA damage and proficient non-homologous end-joining (NHEJ)-directed DNA repair by catalyzing the di- and trimethylation of 'Lys-20' of histone H4. May play a role in class switch reconbination by catalyzing the di- and trimethylation of 'Lys-20' of histone H4.
  • 基因功能参考文献：
    1. The analysis of human pancreatic cancer biopsies was concordant with these findings, because high levels of SUV420H2 correlated with a loss of epithelial characteristics in progressively invasive cancer. Together, these data indicate that SUV420H2 is an upstream epigenetic regulator of epithelial/mesenchymal state control. PMID: 29229751
    2. Study provides evidence that Suv420h2 controls the H4K20 methylome of osteoblasts and is critical for normal progression of osteoblastogenesis. PMID: 27862226
    3. Altogether, these results reveal Suv4-20h-mediated histone H4K20 tri-methylation as a critical determinant in the selection of active replication initiation sites in heterochromatin regions of mammalian genomes. PMID: 28778956
    4. The sequences surrounding both methylation sites do not fit to the specificity profile of SUV4-20H1. PMID: 27105552
    5. Upregulation of long non-coding RNA PAPAS in response to hypoosmotic stress does not increase H4K20me3 because of Nedd4-dependent ubiquitinylation and proteasomal degradation of Suv4-20h2. PMID: 26904956
    6. One of the most downregulated genes in response to SUV420H2 expression was the Src substrate, tensin-3, a focal adhesion protein that contributes to cancer cell migration. PMID: 25814362
    7. The crystal structure of SUV420H2 was used to characterize substrate selectivity and product specificity. PMID: 24396869
    8. SUV420H1 and SUV420H2 isoforms have different in their cellular localization and effects on myogenic differentiation PMID: 21206904
    9. The decrease in trimethylation of lysine 20 of histone H4 in breast cancer cells was accompanied by diminished expression of Suv4-20h2 histone methyltransferase. PMID: 16322686
    10. The data indicate that Suv4-20 generates nearly ubiquitous dimethylation that facilitates the DNA damage response and selective trimethylation that is involved in heterochromatin formation.[Suv4-20] PMID: 18296440
    11. occurrence of an unusual TG 3' splice site in intron 5 PMID: 17672918

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  • 亚细胞定位：
    Nucleus. Chromosome.
  • 蛋白家族：
    Class V-like SAM-binding methyltransferase superfamily, Histone-lysine methyltransferase family, Suvar4-20 subfamily
  • 数据库链接：

    HGNC: 28405

    OMIM: 613198

    KEGG: hsa:84787

    STRING: 9606.ENSP00000255613

    UniGene: Hs.590982