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  4. Recombinant Human Kinesin-like protein KIF15 (KIF15), partial

Recombinant Human Kinesin-like protein KIF15 (KIF15), partial

  • 货号：
    CSB-YP868310HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP868310HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP868310HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP868310HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP868310HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    KIF15
  • Uniprot No.：
  • 别名：
    KIF15; KLP2; KNSL7Kinesin-like protein KIF15; Kinesin-like protein 2; hKLP2; Kinesin-like protein 7; Serologically defined breast cancer antigen NY-BR-62
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Plus-end directed kinesin-like motor enzyme involved in mitotic spindle assembly.
  • 基因功能参考文献：
    1. KIF15 knockdown using short hairpin RNA in two human lung adenocarcinoma cell lines induced G1/S phase cell cycle arrest and inhibited cell growth. PMID: 30439711
    2. The non-motor microtubule-binding tail domain interacts with the microtubule's E-hook tail with a rupture force higher than the stall force of the motor. This allows Kif15 dimers to productively and efficiently generate forces that could potentially slide microtubules apart. PMID: 28918951
    3. Both Kif15 and KBP are required for the alignment of all the chromosomes to the metaphase plate and the assembly of stable kinetochore fibers of the correct length. PMID: 28445502
    4. This study identified KIF15 as a critical regulator that promotes pancreatic cancer proliferation, broadening our understanding of KIF15 function in tumorigenesis. PMID: 28595260
    5. To our knowledge, KIF15 is the first kinesin to be associated with congenital thrombocytopenia. PMID: 28150392
    6. KIF15 is involved in alternative spindle assembly pathways. PMID: 27091450
    7. When intersecting microtubules are parallel, Kif15 motors can drive (biased) parallel sliding because the motor simultaneously steps on both microtubules that it cross-links. PMID: 26969727
    8. These data reveal that hKif15 is a plus-end-directed processive homotetramer that can step against loads of up to 3.5 pN. PMID: 24668168
    9. Eg5, Kif15, and dynein work together to build a bipolar spindle and reveal an important role for antagonistic motors in chromosome segregation. PMID: 25127142
    10. regulates alpha2 integrin internalization PMID: 24659801
    11. Although Kif15 contains ADP in the catalytic site, its motor-domain structure was captured in the ;ATP-like' configuration, with the neck linker docked to the catalytic core. PMID: 24419385
    12. By examining Kif15 activity in two cellular contexts, it was found that Kif15 bound to kinetochore fibers antagonizes centrosome separation while Kif15 bound to nonkinetochore MTs mediates centrosome separation. PMID: 23791727
    13. Pole separation depends on Hklp2/Kif15, an otherwise dispensable plus end-directed spindle motor and results in spindles with two centrosomal poles. PMID: 22024925
    14. Kif15 can replace all essential functions of Eg5 in bipolar spindle assembly. PMID: 19818618
    15. Hklp2 participates in the assembly and stabilization of the bipolar spindle. PMID: 19818619

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  • 亚细胞定位：
    Cytoplasm. Cytoplasm, cytoskeleton, spindle.
  • 蛋白家族：
    TRAFAC class myosin-kinesin ATPase superfamily, Kinesin family, KLP2 subfamily
  • 组织特异性：
    Expressed in testis, colon, thymus and in breast cancer.
  • 数据库链接：

    HGNC: 17273

    OMIM: 617569

    KEGG: hsa:56992

    STRING: 9606.ENSP00000324020

    UniGene: Hs.658939