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  4. Recombinant Human LIM/homeobox protein Lhx3 (LHX3)

Recombinant Human LIM/homeobox protein Lhx3 (LHX3)

  • 中文名称：
    人LHX3重组蛋白
  • 货号：
    CSB-YP871386HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人LHX3重组蛋白
  • 货号：
    CSB-EP871386HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人LHX3重组蛋白
  • 货号：
    CSB-EP871386HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人LHX3重组蛋白
  • 货号：
    CSB-BP871386HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人LHX3重组蛋白
  • 货号：
    CSB-MP871386HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    LHX3
  • Uniprot No.：
  • 别名：
    CPHD 3; CPHD3; DKFZp762A2013; LHX 3; LHX3; LHX3_HUMAN; LIM 3; LIM homeobox 3; LIM homeobox gene 3; LIM homeobox protein 3; LIM/homeobox protein Lhx3; LIM/homeodomain protein LHX3; Lim3; M2 LHX3; mLim-3; mLIM3; P LIM
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-397
  • 氨基酸序列
    MLLETGLERD RARPGAAAVC TLGGTREIPL CAGCDQHILD RFILKALDRH WHSKCLKCSD CHTPLAERCF SRGESVYCKD DFFKRFGTKC AACQLGIPPT QVVRRAQDFV YHLHCFACVV CKRQLATGDE FYLMEDSRLV CKADYETAKQ REAEATAKRP RTTITAKQLE TLKSAYNTSP KPARHVREQL SSETGLDMRV VQVWFQNRRA KEKRLKKDAG RQRWGQYFRN MKRSRGGSKS DKDSVQEGQD SDAEVSFPDE PSLAEMGPAN GLYGSLGEPT QALGRPSGAL GNFSLEHGGL AGPEQYRELR PGSPYGVPPS PAAPQSLPGP QPLLSSLVYP DTSLGLVPSG APGGPPPMRV LAGNGPSSDL STGSSGGYPD FPASPASWLD EVDHAQF
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Acts as a transcriptional activator. Binds to and activates the promoter of the alpha-glycoprotein gene, and synergistically enhances transcription from the prolactin promoter in cooperation with POU1F1/Pit-1. Required for the establishment of the specialized cells of the pituitary gland and the nervous system. Involved in the development of interneurons and motor neurons in cooperation with LDB1 and ISL1.
  • 基因功能参考文献：
    1. Study did not identify HESX1 and LHX3 mutations by Sanger in brazilian patients with combined pituitary hormone deficiency PMID: 28734020
    2. Further functional studies show that forced expression of LHX3 in lung cancer cells obviously promotes cell proliferation and invasion, whereas inhibits cell apoptosis. In summary, LHX3 is an early-stage and radiosensitivity prognostic biomarker, and a novel potential oncogene in lung adenocarcinoma . PMID: 28731174
    3. investigated the specific mutations in PROP1, POU1F1, LHX3, and HESX1 genes in patients with combined pituitary hormone deficiency (CPHD) in Turkey PMID: 25500790
    4. LHX3 is upregulated in high-grade oligodendroglioma. PMID: 25399296
    5. The downstream enhancer region of LHX3 in regulating gene expression at the cellular level during development, is reported. PMID: 24100213
    6. Data suggest that low serum FSH (follicle stimulating hormone) levels in men with SNP in promoter region of FSHB (FSH beta subunit; -211G/T) result from reduced LHX3 binding to FSHB promoter and down-regulation of FSHB transcription in gonadotrophs. PMID: 23766128
    7. The present study was unable to confirm a significant association of all of the three SNPs, rs12338076 in LHX3-QSOX2, and rs1457595 and rs17032362 in IGF1, with adult height in our study population. PMID: 22503243
    8. descriprion of pediatric patients with combined pituitary hormone deficiency with a novel mutation in LHX3; the T194R mutation affects a critical residue in the LHX3 protein; study extends understanding of phenotypic features, molecular mechanism and developmental course associated with mutations in the LHX3 gene PMID: 22286346
    9. This study of the first nonconsanguineous patient with LHX3 mutations demonstrates the pleiotropic roles of LHX3 during development and its full involvement in the complex disease phenotype of syndromic combined pituitary hormone deficiency. PMID: 22238406
    10. This study establishes ISL1 as a novel transcriptional regulator of LHX3 and describes a potential mechanism for regulation by PITX1. PMID: 22194342
    11. LHX3 mutations are a rare cause of hypopituitarism. We report on a patient with a novel LHX3 mutation in exon 2 with the phenotype of combined pituitary hormone deficiency with short neck and sensorineural hearing impairment. [review] PMID: 21249393
    12. A genome-wide association study in 19 633 Japanese subjects identified LHX3-QSOX2 and IGF1 as adult height loci. PMID: 20189936
    13. Mutations in LHX3 are associated with a short neck combination with GHD (growth hormone deficiecy), PRL(prolactin) and TSH (thyroid stimulating hormone) and gonadotropin deficiency p. 278 PMID: 14646405
    14. Mutations within LHX3 are associated with recessive combined pituitary hormone deficiency, with sparing of cortisol secretion... P. 207 PMID: 14714741
    15. LHX3 LIM homeodomain transcription factor is involved in activation of the FSH beta-subunit gene in the pituitary gonadotrope cell. PMID: 15271874
    16. identified unique amino acids within LHX3 that are important for its transcriptional activity and are phosphorylated PMID: 15517599
    17. LHX3 is expressed at all stages of early development. PMID: 15567726
    18. Specificity protein 1 is a regulator of both promoters through interaction with GC boxes and also, a distal element within intron 1a that is recognized by nuclear factor I is critical for hLHX3b promoter function. PMID: 16179410
    19. The presence of a hypointense pituitary lesion and other clinical findings broadens the phenotype associated with LHX3 gene mutation. PMID: 16394081
    20. Lhx3expression of Isl-1 and Lhx3, together with steroidogenic factor 1 (SF-1), culminates in the activation of both the rat as well as human GnRH-R promoter, suggesting that this combination is evolutionarily conserved among mammals PMID: 16613990
    21. in 7 combined pituitary hormone deficiency patients from 4 consanguineous pedigrees, 4 novel, recessive mutations were identified: a deletion of the entire gene, mutations causing truncated proteins & a mutation causing a substitution in the homeodomain PMID: 17327381
    22. LHX3 mutations is associated with sensorineural hearing loss and interaction between LHX3 and SOX2 may contribute to the development of the inner ear and the anterior pituitary. PMID: 18407919
    23. Although the LIM interaction domain of Ldb1 (Ldb1(LID)) and Isl1(LBD) share low levels of sequence homology, X-ray and NMR structures reveal that they bind Lhx3 in an identical manner, that is, Isl1(LBD) mimics Ldb1(LID). PMID: 18583962
    24. Existence of cervical vertebral malformations are revealed, responsible for the rigid neck and the development of scoliosis in LIM3 mutated patients. PMID: 19126629
    25. The Lhx3 gene encodes two isoforms, LHX3a and LHX3b, that differ in their amino-terminal sequences. A novel LHX3 protein (M2-LHX3) is identified and it is determined that this molecule is generated by an internal translation initiation codon. PMID: 11470784

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  • 相关疾病：
    Pituitary hormone deficiency, combined, 3 (CPHD3)
  • 亚细胞定位：
    Nucleus.
  • 数据库链接：

    HGNC: 6595

    OMIM: 221750

    KEGG: hsa:8022

    STRING: 9606.ENSP00000360811

    UniGene: Hs.148427