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  4. Recombinant Human Mediator of RNA polymerase II transcription subunit 25 (MED25)

Recombinant Human Mediator of RNA polymerase II transcription subunit 25 (MED25)

  • 中文名称：
    人MED25重组蛋白
  • 货号：
    CSB-YP741144HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人MED25重组蛋白
  • 货号：
    CSB-EP741144HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人MED25重组蛋白
  • 货号：
    CSB-EP741144HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人MED25重组蛋白
  • 货号：
    CSB-BP741144HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人MED25重组蛋白
  • 货号：
    CSB-MP741144HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    MED25
  • Uniprot No.：
  • 别名：
    ACID1; Activator interaction domain 1 protein; Activator interaction domain-containing protein 1; Activator-recruited cofactor 92 kDa component; ARC/mediator transcriptional coactivator subunit ; ARC/mediator transcriptional coactivator subunit ARC92; ARC92; BVSYS; CMT2B2; DKFZp434K0512; FLJ10193; med25; MED25 protein; MED25_HUMAN; Mediator complex subunit 25; Mediator of RNA polymerase II transcription subunit 25; Mediator of RNA polymerase II transcription; subunit 25 homolog (S cerevisiae); Mediator of RNA polymerase II transcription; subunit 25 homolog (yeast); MGC70671; p78; prostate-derived protein 78 kDa; PTOV2; TCBAP0758
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-747
  • 氨基酸序列
    MVPGSEGPAR AGSVVADVVF VIEGTANLGP YFEGLRKHYL LPAIEYFNGG PPAETDFGGD YGGTQYSLVV FNTVDCAPES YVQCHAPTSS AYEFVTWLDG IKFMGGGGES CSLIAEGLST ALQLFDDFKK MREQIGQTHR VCLLICNSPP YLLPAVESTT YSGCTTENLV QQIGERGIHF SIVSPRKLPA LRLLFEKAAP PALLEPLQPP TDVSQDPRHM VLVRGLVLPV GGGSAPGPLQ SKQPVPLPPA APSGATLSAA PQQPLPPVPP QYQVPGNLSA AQVAAQNAVE AAKNQKAGLG PRFSPITPLQ QAAPGVGPPF SQAPAPQLPP GPPGAPKPPP ASQPSLVSTV APGSGLAPTA QPGAPSMAGT VAPGGVSGPS PAQLGAPALG GQQSVSNKLL AWSGVLEWQE KPKPASVDAN TKLTRSLPCQ VYVNHGENLK TEQWPQKLIM QLIPQQLLTT LGPLFRNSRM VQFHFTNKDL ESLKGLYRIM GNGFAGCVHF PHTAPCEVRV LMLLYSSKKK IFMGLIPYDQ SGFVNGIRQV ITNHKQVQQQ KLEQQQRGMG GQQAPPGLGP ILEDQARPSQ NLLQLRPPQP QPQGTVGASG ATGQPQPQGT AQPPPGAPQG PPGAASGPPP PGPILRPQNP GANPQLRSLL LNPPPPQTGV PPPQASLHHL QPPGAPALLP PPHQGLGQPQ LGPPLLHPPP AQSWPAQLPP RAPLPGQMLL SGGPRGPVPQ PGLQPSVMED DILMDLI
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for RARA/RXRA-mediated transcription.
  • 基因功能参考文献：
    1. FOS strongly binds to the same MED25 site as ETV4 activation domain and JUN interacts with the other two MED25 sites. PMID: 28728983
    2. Med25, a variable member of the Mediator complex, is a coactivator of ligand-activated ERalpha that interacts with ERalpha through its C-terminal LXXLL motif after BPA exposure, and is functionally involved in BPA-induced transcriptional regulation of CYP2C9 expression and enzyme activity. PMID: 27273787
    3. the critical region of chromosome 19 a homozygous variant (c.418C>T, p.Arg140Trp) in Mediator complex subunit 25 predicted as deleterious. PMID: 25527630
    4. A model is proposed in which the N-terminal transactivation domain of ERM binds the MED25 VP16 H1 binding pocket in a helical conformation. PMID: 26130716
    5. Homozygous MED25 mutation implicated in eye-intellectual disability syndrome. PMID: 25792360
    6. MED25 is important for regulating the epigenetic landscape resulting in transcriptional activation of a highly inducible gene, CYP2C9 PMID: 25391650
    7. Med25 is identified as a new coactivator of ERalpha that is required for ERalpha-mediated regulation of CYP2C9 expression PMID: 24960263
    8. Mediator subunit MED25 plays a critical role in this process and identify a MED25 domain that serves as a docking site on Mediator for the ATF6alpha transcription activation domain. PMID: 23864652
    9. the identification of MED25 as one of the HNF4alpha binding partners in pancreatic ss-cells leading to insulin secretion which is impaired in MODY patients PMID: 22952853
    10. solution NMR structure of protein MED25(391-543), comprising the activator interacting domain of subunit 25 of mediator, is presented with the measurement of polypeptide backbone heteronuclear 15N-{1H} NOEs to identify fast internal motional modes PMID: 21785987
    11. The regulatory interaction between Sox9, Wwp2 and Med25 defines the Sox9 transcriptional mechanisms of chondrogenesis in the forming palate. PMID: 21427722
    12. Preliminary NMR chemical shift mapping showed that VP16 H2 (VP16C) interacts with MED25 ACID through one face of the beta-barrel, defined by strands B4-B7-B6. PMID: 20974256
    13. A hydrophobic furrow, formed by a beta-barrel and two alpha-helices in MED25 VBD, interacts tightly with VP16 TADn PMID: 21378963
    14. report the NMR structure of the Mediator subunit Med25 (also called Arc92) activator interaction domain (ACID) and analyze the structural and functional interaction of ACID with the archetypical acidic transcription activator VP16 PMID: 21378965
    15. These results define a role for Med25 and the Mediator complex in the regulation of xenobiotic metabolism and lipid homeostasis. PMID: 21135126
    16. Lana-1-induced serum response element activation was inhibited by the dominant-negative N-terminal domain of the human MED25 mediator subunit, suggesting that this subunit mediates Lana-1-induced serum response element activation. PMID: 20089804
    17. cloned a prostate-derived cDNA encoding a novel protein with a predicted molecular weight of 78 kDa (P(78)), and precisely mapped the cDNA sequence to chromosome 19 PMID: 12163014
    18. biochemical purification and cloning of ARC92; identified as a specific cellular interaction partner of the VP16 activation domain PMID: 14983011
    19. data suggest that MED25, in cooperation with CBP and Mediators through its distinct domains, imposes a selective advantage on RAR/RXR activation PMID: 17641689
    20. Data show that Med25 is coordinately expressed with Pmp22 gene dosage and expression in transgenic mice and rats, and suggest a potential role of this protein in the molecular etiology of Charcot-Marie-Tooth disease. PMID: 19290556

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  • 相关疾病：
    Charcot-Marie-Tooth disease 2B2 (CMT2B2); Basel-Vanagaite-Smirin-Yosef syndrome (BVSYS)
  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    Mediator complex subunit 25 family
  • 组织特异性：
    Ubiquitously expressed. Highest levels in brain, heart, kidney, peripheral leukocytes, placenta, skeletal muscle and spleen.
  • 数据库链接：

    HGNC: 28845

    OMIM: 605589

    KEGG: hsa:81857

    STRING: 9606.ENSP00000326767

    UniGene: Hs.656639