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  4. Recombinant Human Methionine adenosyltransferase 2 subunit beta (MAT2B)

Recombinant Human Methionine adenosyltransferase 2 subunit beta (MAT2B)

  • 货号：
    CSB-YP882157HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP882157HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP882157HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP882157HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP882157HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    1110064C04Rik; 2410018D16Rik; AI182287; AU022853; Beta regulatory subunit of methionine adenosyltransferase; dTDP 4 keto 6 deoxy D glucose 4 reductase; DTDP-4-keto-6-deoxy-D-glucose 4-reductase; MAT II; MAT II beta; Mat2b; MAT2B_HUMAN; MATIIbeta; Methionine adenosyltransferase 2 beta subunit; Methionine adenosyltransferase 2 subunit beta; Methionine adenosyltransferase II beta; MGC12237; MSTP045; Nbla02999; OTTMUSP00000005600; Putative dTDP-4-keto-6-deoxy-D-glucose 4-reductase; Putative protein product of Nbla02999; RP23-382C18.2; SDR23E1; Short chain dehydrogenase/reductase family 23E member 1; TGR
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-334
  • 氨基酸序列
    VGREKELSI HFVPGSCRLV EEEVNIPNRR VLVTGATGLL GRAVHKEFQQ NNWHAVGCGF RRARPKFEQV NLLDSNAVHH IIHDFQPHVI VHCAAERRPD VVENQPDAAS QLNVDASGNL AKEAAAVGAF LIYISSDYVF DGTNPPYREE DIPAPLNLYG KTKLDGEKAV LENNLGAAVL RIPILYGEVE KLEESAVTVM FDKVQFSNKS ANMDHWQQRF PTHVKDVATV CRQLAEKRML DPSIKGTFHW SGNEQMTKYE MACAIADAFN LPSSHLRPIT DSPVLGAQRP RNAQLDCSKL ETLGIGQRTP FRIGIKESLW PFLIDKRWRQ TVFH
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Regulatory subunit of S-adenosylmethionine synthetase 2, an enzyme that catalyzes the formation of S-adenosylmethionine from methionine and ATP. Regulates MAT2A catalytic activity by changing its kinetic properties, increasing its affinity for L-methionine. Can bind NADP (in vitro).
  • 基因功能参考文献：
    1. Results show that MAT2B expression is frequently upregulated in malignant melanoma tissues. MAT2B downregulation delays tumor growth in vivo, and suppressed cell growth, colony formation ability and induced apoptosis in vitro indicating that MAT2B is critical for melanoma cell proliferation and tumorigenicity. PMID: 27573889
    2. Interaction between MAT2B and GIT1 serves as a scaffold and facilitates signaling in multiple steps of the Ras/Raf/MEK/ERK pathway PMID: 25794709
    3. Mutagenesis of MATalpha2 and MATbeta phospho-sites destabilized them and prevented hepatic stellate cell trans-differentiation. PMID: 25294683
    4. MAT2B and GIT1 form a scaffold, which recruits and activates MEK and ERK to promote growth and tumorigenesis. PMID: 23325601
    5. The structure of MAT2B reveals a short-chain dehydrogenase/reductase (SDR) core with specificity for the NADP/H cofactor, and harbors the SDR catalytic triad. PMID: 23425511
    6. Coexpression of MAT2A and MAT2B in COS-1 cells resulted in significantly increased MAT enzyme activity. PMID: 21813468
    7. Data show MAT2beta variants reside mostly in the nucleus and regulate HuR subcellular content to affect cell proliferation. PMID: 20421296
    8. Lower expression of both MAT2A and MAT2beta and interfere with leptin signaling in liver cancer cells. PMID: 18041713
    9. Splicing variants of MAT2beta are up-regulated in hepatocellular carcinoma and regulated tumor cell growth and death. PMID: 18045590
    10. a switch in MAT expression in liver cancer was accompanied by increasing expression of MAT2B PMID: 18698677
    11. differences in malignant cell survival, effected by MAT-IIbeta ablation, suggest that it may be possible to use this approach to disadvantage leukemic cell survival in vivo with little to no harm to normal cells. PMID: 18753136

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  • 蛋白家族：
    DTDP-4-dehydrorhamnose reductase family, MAT2B subfamily
  • 组织特异性：
    Widely expressed.
  • 数据库链接：

    HGNC: 6905

    OMIM: 605527

    KEGG: hsa:27430

    STRING: 9606.ENSP00000325425

    UniGene: Hs.54642