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  4. Recombinant Human Nucleolar and coiled-body phosphoprotein 1 (NOLC1)

Recombinant Human Nucleolar and coiled-body phosphoprotein 1 (NOLC1)

  • 货号：
    CSB-YP015929HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP015929HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP015929HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP015929HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP015929HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    NOLC1
  • Uniprot No.：
  • 别名：
    140 kDa nucleolar phosphoprotein; HCV NS5A trans regulated protein 13; HCV NS5A transactivated protein 13; HCV NS5A-transactivated protein 13; Hepatitis C virus NS5A transactivated protein 13; Hepatitis C virus NS5A-transactivated protein 13; KIAA0035; NOLC 1; NOLC1; NOLC1_HUMAN; NOPP 130; NOPP 140; NOPP130; Nopp140; NS5ATP13; Nucleolar 130 kDa protein; Nucleolar and coiled body phosphoprotein 1; Nucleolar and coiled body phosphprotein 1; Nucleolar and coiled-body phosphoprotein 1; Nucleolar phosphoprotein p130; Nucleolar protein p130; P130
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-699
  • 氨基酸序列
    MADAGIRRVV PSDLYPLVLG FLRDNQLSEV ANKFAKATGA TQQDANASSL LDIYSFWLKS AKVPERKLQA NGPVAKKAKK KASSSDSEDS SEEEEEVQGP PAKKAAVPAK RVGLPPGKAA AKASESSSSE ESSDDDDEED QKKQPVQKGV KPQAKAAKAP PKKAKSSDSD SDSSSEDEPP KNQKPKITPV TVKAQTKAPP KPARAAPKIA NGKAASSSSS SSSSSSSDDS EEEKAAATPK KTVPKKQVVA KAPVKAATTP TRKSSSSEDS SSDEEEEQKK PMKNKPGPYS SVPPPSAPPP KKSLGTQPPK KAVEKQQPVE SSEDSSDESD SSSEEEKKPP TKAVVSKATT KPPPAKKAAE SSSDSSDSDS SEDDEAPSKP AGTTKNSSNK PAVTTKSPAV KPAAAPKQPV GGGQKLLTRK ADSSSSEEES SSSEEEKTKK MVATTKPKAT AKAALSLPAK QAPQGSRDSS SDSDSSSSEE EEEKTSKSAV KKKPQKVAGG AAPSKPASAK KGKAESSNSS SSDDSSEEEE EKLKGKGSPR PQAPKANGTS ALTAQNGKAA KNSEEEEEEK KKAAVVVSKS GSLKKRKQNE AAKEAETPQA KKIKLQTPNT FPKRKKGEKR ASSPFRRVRE EEIEVDSRVA DNSFDAKRGA AGDWGERANQ VLKFTKGKSF RHEKTKKKRG SYRGGSISVQ VNSIKFDSE
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Nucleolar protein that acts as a regulator of RNA polymerase I by connecting RNA polymerase I with enzymes responsible for ribosomal processing and modification. Required for neural crest specification: following monoubiquitination by the BCR(KBTBD8) complex, associates with TCOF1 and acts as a platform to connect RNA polymerase I with enzymes responsible for ribosomal processing and modification, leading to remodel the translational program of differentiating cells in favor of neural crest specification. Involved in nucleologenesis, possibly by playing a role in the maintenance of the fundamental structure of the fibrillar center and dense fibrillar component in the nucleolus. It has intrinsic GTPase and ATPase activities.
  • 基因功能参考文献：
    1. In this study, the disordered feature of Nopp140 and the effect of CK2alpha on the structure of Nopp140 were examined using single-molecule fluorescence resonance energy transfer (smFRET) and electron paramagnetic resonance (EPR). PMID: 27297113
    2. D120 and R195 of H5N1 influenza virus NS1 were crucial for the binding to host NOLC1. PMID: 25645906
    3. pRb2/p130 cytoplasmic delocalization can lead to cell cycle deregulation PMID: 25205458
    4. our study demonstrated that DNA methylation is a key mechanism of silenced NOLC1 expression in human hepatocellular carcinoma cells, and NOLC1 gene hypermethylation of the four CpG dinucleotides is a potential biomarker for hepatocellular carcinoma. PMID: 23970161
    5. A Nopp140 fragment (residues 568-596) and inositol hexakisphosphate competitively bind to the catalytic subunit of CK2 (CK2alpha), and phospho-Ser574 of Nopp140 significantly enhances its interaction with CK2alpha. PMID: 24218616
    6. H5N1 viral NS1 interacts with host NOLC1 protein. PMID: 23188192
    7. Structural disorder and local order of hNopp140 PMID: 22906532
    8. The regulation of NOCL1 protein is a critical role for the transcription NF-kB and cAMP response element-bing protein PMID: 21266110
    9. NOPP140 is involved in a PKA signaling pathway that leads to agp activation PMID: 12167624
    10. These data show that Nopp140 reversibly associates with snoRNPs in a phosphorylation-dependent manner, but this association has no effect on pseudouridylation of rRNA. PMID: 12446766
    11. Hypoxia induces substantial p130 dephosphorylation and nuclear accumulation, leading to the formation of E2F4/p130 complexes and increased occupancy of E2F4 and p130 at the RAD51 and BRCA1 promoters. PMID: 17001309
    12. hNopp140 serves as a negative regulator of CK2 and InsP(6) stimulates the activity of CK2 by blocking the interaction between hNopp140 and CK2 PMID: 17038328
    13. Evolutionarily conserved multisubunit protein complex that contains p130 and E2F4 mediates the repression of cell cycle-dependent genes in quiescence. PMID: 17531812
    14. hNopp140, a scaffold protein, is involved in the nucleolar assembly, fusion, and maintenance PMID: 18253863
    15. InsP(6) specifically binds to CK2alpha and disrupts the interaction between CK2alpha and Nopp140 with an IC(50) value of 25 microM, thereby attenuating the Nopp140-mediated repression of CK2 activity. PMID: 18790693
    16. The functional deficiency in spinal muscular atrophy cells is associated with decreased localization of the snoRNP chaperone Nopp140 in Cajal bodies that correlates with disease severity. PMID: 19129172
    17. Nopp140 could be a component implicated in the early steps of pre-rRNA processing. PMID: 19381672
    18. both NOLC1 and tumor protein 53 work together synergistically to activate the MDM2 promoter in NPC cells. PMID: 19541936

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  • 亚细胞定位：
    Nucleus, nucleolus. Cytoplasm.
  • 数据库链接：

    HGNC: 15608

    OMIM: 602394

    KEGG: hsa:9221

    STRING: 9606.ENSP00000359024

    UniGene: Hs.523238