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  4. Recombinant Human Probable methyltransferase BCDIN3D (BCDIN3D)

Recombinant Human Probable methyltransferase BCDIN3D (BCDIN3D)

  • 货号：
    CSB-YP767206HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP767206HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP767206HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP767206HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP767206HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    BCDIN3D
  • Uniprot No.：
  • 别名：
    BCDIN3 domain containing; BCDIN3 domain-containing protein; BCDIN3D; BN3D2_HUMAN; Probable methyltransferase BCDIN3D
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-292
  • 氨基酸序列
    MAVPTELDGG SVKETAAEEE SRVLAPGAAP FGNFPHYSRF HPPEQRLRLL PPELLRQLFP ESPENGPILG LDVGCNSGDL SVALYKHFLS LPDGETCSDA SREFRLLCCD IDPVLVKRAE KECPFPDALT FITLDFMNQR TRKVLLSSFL SQFGRSVFDI GFCMSITMWI HLNHGDHGLW EFLAHLSSLC HYLLVEPQPW KCYRAAARRL RKLGLHDFDH FHSLAIRGDM PNQIVQILTQ DHGMELICCF GNTSWDRSLL LFRAKQTIET HPIPESLIEK GKEKNRLSFQ KQ
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    O-methyltransferase that specifically monomethylates 5'-monophosphate of cytoplasmic histidyl tRNA (tRNA(His)), acting as a capping enzyme by protecting tRNA(His) from cleavage by DICER1. Also able, with less efficiently, to methylate the 5' monophosphate of a subset of pre-miRNAs, acting as a negative regulator of miRNA processing. The 5' monophosphate of pre-miRNAs is recognized by DICER1 and is required for pre-miRNAs processing: methylation at this position reduces the processing of pre-miRNAs by DICER1. Was also reported to mediate dimethylation of pre-miR-145; however dimethylation cannot be reproduced by another group which observes a monomethylation of pre-miR-145.
  • 基因功能参考文献：
    1. BCDIN3D might serve as an important prognostic factor for triple-negative breast cancer PMID: 27259993
    2. BCDIN3D acts as a cytoplasmic tRNAHis-specific 5-methylphosphate capping enzyme. PMID: 28119416
    3. Human BCDIN3D monomethylates the 5'-monophosphate of cytoplasmic tRNAHis in vitro. In BCDN3D-knockout cells, established byCRISPR/Cas9 editing, the methyl moiety at the 5'-monophosphate of cytoplasmic tRNAHis is lost, and the exogenous expression of BCDIN3D in the knockout cells restores the modification in cytoplasmic tRNAHis. PMID: 28119416
    4. BCDIN3D depletion leads to lower pre-miR-145 and concomitantly increased mature miR-145 levels in breast cancer cells, which suppresses their tumorigenic phenotypes. PMID: 23063121
  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Methyltransferase superfamily
  • 数据库链接：

    HGNC: 27050

    KEGG: hsa:144233

    STRING: 9606.ENSP00000335201

    UniGene: Hs.142736