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  4. Recombinant Human Protein phosphatase 1 regulatory subunit 14A (PPP1R14A)

Recombinant Human Protein phosphatase 1 regulatory subunit 14A (PPP1R14A)

  • 货号：
    CSB-YP850252HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP850252HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP850252HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP850252HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP850252HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    17 kDa PKC-potentiated inhibitory protein of PP1; 17-KDa protein; 17kDa PKC potentiated inhibitory protein of PP1; CPI 17 alpha; CPI 17; CPI 17alpha; CPI-17; CPI17 alpha; CPI17; PKC potentiated inhibitory protein of PP1; PKC potentiated inhibitory protein of PP1, 17-KD; PP14A_HUMAN; PPP1INL; Ppp1r14a; Protein kinase C-potentiated inhibitor of protein phosphatase 1, 17-KD; Protein kinase C-potentiated inhibitor protein of 17 kDa; Protein phosphatase 1; Protein phosphatase 1 regulatory (inhibitor) subunit 14A; Protein phosphatase 1 regulatory subunit 14A; Regulatory subunit 14A
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-147
  • 氨基酸序列
    MAAQRLGKRV LSKLQSPSRA RGPGGSPGGL QKRHARVTVK YDRRELQRRL DVEKWIDGRL EELYRGMEAD MPDEINIDEL LELESEEERS RKIQGLLKSC GKPVEDFIQE LLAKLQGLHR QPGLRQPSPS HDGSLSPLQD RARTAHP
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Inhibitor of PPP1CA. Has over 1000-fold higher inhibitory activity when phosphorylated, creating a molecular switch for regulating the phosphorylation status of PPP1CA substrates and smooth muscle contraction.
  • 基因功能参考文献：
    1. CPI-17 drives Ras activity and tumorigenesis in melanomas in a two-fold way; inactivation of the tumor suppressor merlin and activation of the growth promoting ERM family. PMID: 27793041
    2. The s call the mutual sequestration mechanism through which pCPI-17 and myosin light-chain phosphatase interact inhibition by unfair competition: myosin light-chain phosphatase protects pCPI-17 from other phosphatases, while pCPI-17 blocks other substrates from the active site of myosin light-chain phosphatase. PMID: 28387646
    3. Study finds that CPI-17 (PPP1R14A protein phosphatase 1 regulatory inhibitor subunit 14A, 17 kDa) is not expressed in the healthy peripheral nervous system or in nontumour pathologies of the peripheral nervous system. CPI-17 up-regulation, however, is observed in over 90% of schwannomas, but not in neurofibroma and only rarely in malignant peripheral nerve sheath tumour. PMID: 27248983
    4. CPI-17 has a role in restoring skin homoeostasis in cutaneous field of cancerization lesions PMID: 23800065
    5. analysis of the interaction between plakoglobin and CPI-17, which is affected by the phosphorylation status of CPI-17 in human lung microvascular endothelial cells PMID: 23583905
    6. The N-terminal 21-residue tail domain of CPI-17 is necessary for nuclear localization. Phospho-mimetic Asp-substitution of CPI-17 at Ser12 attenuates the nuclear import. PMID: 23541585
    7. The study characterized the CPI-17 promoter and identified binding sites for GATA-6 and nuclear factor kappa B (NF-kappaB). PMID: 23275439
    8. a novel signaling cascade that links RhoA-mediated calcium sensitivity to MEF2-dependent myocardin expression in VSMCs through a mechanism involving p38 MAPK, PP1alpha, and CPI-17. PMID: 22275376
    9. RACK1 may play a role in PKC/CPI-17 signaling pathway. PMID: 20875397
    10. cAMP/PKA regulates the endothelial barrier via inhibition of the contractile machinery, mainly by the activation of MLCP via inhibition of CPI-17 and RhoA/Rock. PMID: 20202976
    11. mapped to chromosome 19q13.13-q13.2 PMID: 11931393
    12. CPI-17 binds directly to protein kinase C and casein kinase I. PMID: 15003508
    13. importance of PKC/CPI-17-mediated pathway in histamine-triggered cytoskeletal rearrangements leading to lung microvascular barrier compromise. PMID: 15234908
    14. CPI-17 siRNA decreased the level of merlin phosphorylation and consequently Ras and ERK activity in human tumor cell lines PMID: 16885985
    15. These data indicate that 14,15-epoxyeicosatrienoic acid hyperpolarizes airway smooth muscle cells and relaxes precontracted human bronchi and intracellular effects are related to a PKC-dependent process involving a lower phosphorylation level of CPI-17. PMID: 17237191
    16. Human pregnancy is characterized by increases in PKN1 expression and PPP1R14A phosphorylation in the myometrium. PMID: 17301291
    17. These results are consistent with a key molecular role for CPI-17 in airway hyperresponsiveness, in the absence of bronchial wall remodeling. PMID: 18757304
    18. NF2 is rendered inactive by phosphorylation of Ser 518 and this can be explained at least in part by an increased expression of CPI-17. PMID: 18835652

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    PP1 inhibitor family
  • 组织特异性：
    Isoform 1 is detected in aorta and testis. Isoform 2 is detected in aorta.
  • 数据库链接：

    HGNC: 14871

    OMIM: 608153

    KEGG: hsa:94274

    STRING: 9606.ENSP00000301242

    UniGene: Hs.631569