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  4. Recombinant Human Proton-coupled amino acid transporter 1 (SLC36A1), partial

Recombinant Human Proton-coupled amino acid transporter 1 (SLC36A1), partial

  • 中文名称：
    人SLC36A1重组蛋白
  • 货号：
    CSB-YP768756HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人SLC36A1重组蛋白
  • 货号：
    CSB-EP768756HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人SLC36A1重组蛋白
  • 货号：
    CSB-EP768756HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人SLC36A1重组蛋白
  • 货号：
    CSB-BP768756HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人SLC36A1重组蛋白
  • 货号：
    CSB-MP768756HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    SLC36A1
  • Uniprot No.：
  • 别名：
    Dct1; hPAT1; LYAAT1; PAT1; Proton-coupled amino acid transporter 1; Proton/amino acid transporter 1; S36A1_HUMAN; Slc36a1; Solute carrier family 36 member 1; TRAMD3
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Neutral amino acid/proton symporter. Has a pH-dependent electrogenic transport activity for small amino acids such as glycine, alanine and proline. Besides small apolar L-amino acids, it also recognizes their D-enantiomers and selected amino acid derivatives such as gamma-aminobutyric acid.
  • 基因功能参考文献：
    1. A mechanism through which amino acids may suppress the expression of PAT1 on lysosomes by inducing protein cleavage to remove a targeting signal. PMID: 28670736
    2. Data indicate that the glycosylation-deficient mutant of amino acid transporter PAT1 (PAT1(3)(NQ) ) is unstable and is degraded mainly via the endoplasmic reticulum-associated degradation pathway in HEK293 cells. PMID: 28117901
    3. Sertraline is an apparent non-competitive inhibitor of PAT1-mediated transport. PMID: 23962042
    4. Data suggest that PAT1 in enterocytes is responsible for intestinal absorption of some of the alkaloids from areca nut (i.e., arecaidine, guvacine, isoguvacine). PMID: 23488788
    5. estrogen attenuates the activity of PAT1 by directly closing PAT1 PMID: 22975709
    6. In hPAT1 expressing oocytes Gly-Tyr, Gly-Pro, and Gly-Phe inhibited currents induced by drug substances. PMID: 22853447
    7. PATs function as part of an amino acid-sensing engine that drives mTORC1 activation from endosomal and lysosomal membranes PMID: 22574197
    8. In human cells, SLC36A1 localized on intracellular membranes is required for amino acid-dependent mTORC1 activation and cell proliferation. SLC36A1 also has similar in vivo growth regulatory activity to fly SLC36 AAAPs when expressed in Drosophila. PMID: 20498635
    9. PAT1 expressed exclusively in apical membrane of Caco-2 cells. hPAT1 may be responsible for H(+)-coupled amino acid transport expressed in apical membrane of Caco-2 cells. PMID: 12527723
    10. A high-capacity imino acid carrier localized at the small intestinal luminal membrane that transports nutrients and amino acids and imino acids. PMID: 15521011
    11. Amino acid uptake via hPAT1 is inhibited by activators of the cAMP pathway indirectly through inhibition of NHE3 activity. PMID: 15754324
    12. PAT1a, which is 99.0% identical to protein interacting with APP tail 1 (PAT1), is a functional link between the transport and processing of beta-amyloid precursor protein APP and its two paralogs APLP1 and APLP2 in vivo. PMID: 17050537
    13. His-55 might be responsible for binding and translocation of H+ in the course of cellular amino acid uptake by PAT1. PMID: 18230330
    14. This study is the first to demonstrate both taurine uptake via PAT1 and functional expression of PAT1 at the apical membrane of the intestinal epithelium. PMID: 19074966
    15. Data conclude that hPAT1 might be responsible for the intestinal absorption of beta-GPA thereby allowing its oral administration. PMID: 19358571
    16. The role of N-glycosylation in transport function and surface targeting of the human solute carrier PAT1 PMID: 19409386
    17. a disulfide bridge is essential for the transport function of the human proton-coupled amino acid transporter hPAT1 PMID: 19549785

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  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein. Lysosome membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Amino acid/polyamine transporter 2 family
  • 数据库链接：

    HGNC: 18761

    OMIM: 606561

    KEGG: hsa:206358

    STRING: 9606.ENSP00000243389

    UniGene: Hs.269004