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  4. Recombinant Human RAS guanyl-releasing protein 4 (RASGRP4)

Recombinant Human RAS guanyl-releasing protein 4 (RASGRP4)

  • 中文名称：
    人RASGRP4重组蛋白
  • 货号：
    CSB-YP819468HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人RASGRP4重组蛋白
  • 货号：
    CSB-EP819468HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人RASGRP4重组蛋白
  • 货号：
    CSB-EP819468HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人RASGRP4重组蛋白
  • 货号：
    CSB-BP819468HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人RASGRP4重组蛋白
  • 货号：
    CSB-MP819468HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    GRP4_HUMAN; Guanyl nucleotide releasing protein 4; RAS guanyl releasing protein 4; RAS guanyl-releasing protein 4; RASGRP4
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-673
  • 氨基酸序列
    MNRKDSKRKS HQECTGKIGG RGRPRQVRRH KTCPSPREIS KVMASMNLGL LSEGGCSEDE LLEKCIQSFD SAGSLCHEDH MLNMVLAMHS WVLPSADLAA RLLTSYQKAT GDTQELRRLQ ICHLVRYWLM RHPEVMHQDP QLEEVIGRFW ATVAREGNSA QRRLGDSSDL LSPGGPGPPL PMSSPGLGKK RKVSLLFDHL ETGELAQHLT YLEFRSFQAI TPQDLRSYVL QGSVRGCPAL EGSVGLSNSV SRWVQVMVLS RPGPLQRAQV LDKFIHVAQR LHQLQNFNTL MAVTGGLCHS AISRLKDSHA HLSPDSTKAL LELTELLASH NNYARYRRTW AGCAGFRLPV LGVHLKDLVS LHEAQPDRLP DGRLHLPKLN NLYLRLQELV ALQGQHPPCS ANEDLLHLLT LSLDLFYTED EIYELSYARE PRCPKSLPPS PFNAPLVVEW APGVTPKPDR VTLGRHVEQL VESVFKNYDP EGRGTISQED FERLSGNFPF ACHGLHPPPR QGRGSFSREE LTGYLLRASA ICSKLGLAFL HTFHEVTFRK PTFCDSCSGF LWGVTKQGYR CRECGLCCHK HCRDQVKVEC KKRPGAKGDA GPPGAPVPST PAPHASCGSE ENHSYTLSLE PETGCQLRHA WTQTESPHPS WETDTVPCPV MDPPSTASSK LDS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Functions as a cation- and diacylglycerol (DAG)-regulated nucleotide exchange factor activating Ras through the exchange of bound GDP for GTP. May function in mast cells differentiation.
  • 基因功能参考文献：
    1. Ras guanine nucleotide-releasing protein 4 is aberrantly expressed in the fibroblast-like synoviocytes of patients with rheumatoid arthritis and controls their proliferation. PMID: 25330932
    2. Identification of defective isoforms of hRasGRP4 transcripts in rheumatoid arthritis(RA) patients raises the possibility that dysregulated expression of hRasGRP4 in developing monocytes plays a pathogenic role in this subset of RA patients. PMID: 21933395
    3. GPCR activation of Ras and PI3Kc in neutrophils depends on PLCb2/b3 and the RasGEF RasGRP4. PMID: 22728827
    4. RasGRP4 plays a role in myeloid cell signaling growth regulation pathways that are responsive to diacylglycerol levels PMID: 11880369
    5. identification of defective variants of this signaling protein in asthma, mastocytosis, and mast cell leukemia patients and demonstration of the importance of RasGRP4 in mast cell development and function PMID: 11956218
    6. RasGRP4 regulates the expression of prostaglandin D2 in mast cells PMID: 12493770
    7. RasGRP4 translocates to varied intracellular compartments via its DAG/PMA-binding domain to regulate signaling pathways that control gene and protein expression in MCs, including the cell's ability to respond to IL-13. PMID: 18024961
    8. RasGRP4 cooperates with AML1 mutations in T cell leukemogenesis as a class I mutation PMID: 19350351
    9. RasGRP4 is a cation-dependent, guanine nucleotide exchange factor and diacylglycerol/phorbol ester receptor that is selectively expressed in mast cells and their progenitors. RasGRP4 regulates the final stages of mast cell development. PMID: 11956218

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  • 亚细胞定位：
    Cytoplasm. Cell membrane. Note=Recruited to membranes upon activation by DAG.
  • 蛋白家族：
    RASGRP family
  • 组织特异性：
    Expressed by mast cells and their progenitors (at protein level). Specifically expressed in mononuclear leukocytes. Highly expressed in myeloid cells compared to lymphoid cells. Also detected in heart, skeletal muscle, spleen, liver, placenta and lung. No
  • 数据库链接：

    HGNC: 18958

    OMIM: 607320

    KEGG: hsa:115727

    STRING: 9606.ENSP00000465742

    UniGene: Hs.130434