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  4. Recombinant Human RNA-binding protein 8A (RBM8A)

Recombinant Human RNA-binding protein 8A (RBM8A)

  • 中文名称：
    人RBM8A重组蛋白
  • 货号：
    CSB-YP896536HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人RBM8A重组蛋白
  • 货号：
    CSB-EP896536HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人RBM8A重组蛋白
  • 货号：
    CSB-EP896536HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人RBM8A重组蛋白
  • 货号：
    CSB-BP896536HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人RBM8A重组蛋白
  • 货号：
    CSB-MP896536HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    RBM8A
  • Uniprot No.：
  • 别名：
    Binder of OVCA1 1; Binder of OVCA1-1; BOV 1; BOV 1A; BOV 1B; BOV 1C; BOV-1; BOV1; BOV1A; BOV1B; BOV1C; HSPC 114; HSPC114; MDS 014; MDS014; RBM 8; RBM 8A; RBM 8B; RBM8; rbm8a; RBM8A_HUMAN; RBM8B; Ribonucleoprotein RBM 8; Ribonucleoprotein RBM 8A; Ribonucleoprotein RBM8; Ribonucleoprotein RBM8A; RNA binding motif protein 8; RNA binding motif protein 8A; RNA binding motif protein 8B; RNA binding protein 8A; RNA binding protein Y14; RNA-binding motif protein 8A; RNA-binding protein 8A; RNA-binding protein Y14; ZNRP; ZRNP 1; ZRNP1
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-174
  • 氨基酸序列
    ADVLDLHEA GGEDFAMDED GDESIHKLKE KAKKRKGRGF GSEEGSRARM REDYDSVEQD GDEPGPQRSV EGWILFVTGV HEEATEEDIH DKFAEYGEIK NIHLNLDRRT GYLKGYTLVE YETYKEAQAA MEGLNGQDLM GQPISVDWCF VRGPPKGKRR GGRRRSRSPD RRRR
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Required for pre-mRNA splicing as component of the spliceosome. Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). The MAGOH-RBM8A heterodimer inhibits the ATPase activity of EIF4A3, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. The MAGOH-RBM8A heterodimer interacts with the EJC key regulator PYM1 leading to EJC disassembly in the cytoplasm and translation enhancement of EJC-bearing spliced mRNAs by recruiting them to the ribosomal 48S preinitiation complex. Its removal from cytoplasmic mRNAs requires translation initiation from EJC-bearing spliced mRNAs. Associates preferentially with mRNAs produced by splicing. Does not interact with pre-mRNAs, introns, or mRNAs produced from intronless cDNAs. Associates with both nuclear mRNAs and newly exported cytoplasmic mRNAs. The MAGOH-RBM8A heterodimer is a component of the nonsense mediated decay (NMD) pathway. Involved in the splicing modulation of BCL2L1/Bcl-X (and probably other apoptotic genes); specifically inhibits formation of proapoptotic isoforms such as Bcl-X(S); the function is different from the established EJC assembly.
  • 基因功能参考文献：
    1. Findings demonstrate that Y14 nucleoplasmic localization is regulated not only by the previously reported N-terminal localization signal but also by the C-terminal serine/arginine repeat-containing region through phosphorylation and MAGOH binding to Y14. PMID: 29330450
    2. A direct link between Y14 and p53 expression and suggests a function for Y14 in DNA damage signaling. PMID: 28361991
    3. Results demonstrated that RBM8A is highly expressed in hepatocellular carcinoma (HCC), and patients with lower RBM8A expression level had longer overall survival and progression free survival. Also, results indicate that RBM8A promotes invasion and metastasis via epithelial-mesenchymal transition signaling pathway. PMID: 28259942
    4. Y14 may selectively and differentially modulate protein biosynthesis. PMID: 26887951
    5. this data suggests a novel role of RBM8a in the regulation of neurodevelopment. PMID: 26094033
    6. Mutations of RBM8A and TBX6 are associated with disorders of the mullerian ducts. PMID: 25813282
    7. It was demonstrated that Rbm8a is an essential neurogenesis regulator in embryonic cortical development. PMID: 25948253
    8. Depletion of RNA-binding protein RBM8A (Y14) causes cell cycle deficiency and apoptosis in human tumor cells. PMID: 23970407
    9. Y14 positively regulates signals for TNF-alpha-induced IL-6 production at multiple steps beyond an exon junction complex protein. PMID: 23817415
    10. Y14 mutants, which are deficient in binding to Magoh, could still be localized to the nucleus, suggesting the existence of both the nuclear import pathway and function for Y14 unaccompanied by Magoh PMID: 22355610
    11. Data show that Y14 interacts directly with the decapping factor Dcp2 and the 5' cap structure of mRNAs via different but overlapping domains. PMID: 23115303
    12. findings show that the two regulatory SNPs result in diminished RBM8A transcription in vitro and that Y14 expression is reduced in platelets from individuals with thrombocytopenia with absent radii (TAR) PMID: 22366785
    13. Y14 provides a regulatory link between pre-mRNA splicing and snRNP biogenesis. PMID: 21209085
    14. conserved physical linkage in medaka and human genomes PMID: 12054603
    15. Tethered function analysis shows that the Y14/hUpf3b interaction is essential for nonsense-mediated decay, while surprisingly the interaction between hUpf3b and hUpf2 is not. PMID: 12718880
    16. antagonistic post-translational modifications of Y14 may be involved in the remodeling of Y14-containing mRNPs PMID: 16100109
    17. The stable association of multiprotein exon junction complex core with RNA is maintained by inhibition of eIF4AIII ATPase activity by MAGOH-Y14. PMID: 16170325
    18. crystal structure of a tetrameric exon junction core complex containing the DEAD-box adenosine triphosphatase eukaryotic initiation factor 4AIII bound to an ATP analog, MAGOH, Y14, a fragment of MLN51, and a polyuracil mRNA mimic PMID: 16931718
    19. Increased TAP binding correlates with increased SF2/ASF binding, but not increased REF/Aly or Y14 binding. PMID: 18243119
    20. Y14 interacts with STAT3 and regulates the transcriptional activation of STAT3 by influencing the tyrosine-phosphorylation of STAT3. PMID: 18503751
    21. These results indicate that MAGOH regulates the transcriptional activation of STAT3 by interfering complex formation between STAT3 and Y14. PMID: 19254694

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  • 亚细胞定位：
    Nucleus. Nucleus speckle. Cytoplasm.
  • 蛋白家族：
    RBM8A family
  • 组织特异性：
    Ubiquitous.
  • 数据库链接：

    HGNC: 9905

    OMIM: 605313

    KEGG: hsa:9939

    STRING: 9606.ENSP00000333001

    UniGene: Hs.591455