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  4. Recombinant Human SH3 domain-containing kinase-binding protein 1 (SH3KBP1)

Recombinant Human SH3 domain-containing kinase-binding protein 1 (SH3KBP1)

  • 中文名称：
    人SH3KBP1重组蛋白
  • 货号：
    CSB-YP842640HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人SH3KBP1重组蛋白
  • 货号：
    CSB-EP842640HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人SH3KBP1重组蛋白
  • 货号：
    CSB-EP842640HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人SH3KBP1重组蛋白
  • 货号：
    CSB-BP842640HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人SH3KBP1重组蛋白
  • 货号：
    CSB-MP842640HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    c Cbl interacting protein; Cbl interacting protein; Cbl interacting protein of 85 kDa; Cbl-interacting protein of 85 kDa; CD2 binding protein 3; CD2-binding protein 3; CD2BP3; CIN 85; CIN85; GIG 10; GIG10; HSB 1; HSB-1; HSB1; Human Src family kinase binding protein 1; Human Src family kinase-binding protein 1; MIG 18; MIG18; Migration inducing gene 18; Migration inducing gene 18 protein; SH3 domain kinase binding protein 1; SH3 domain-containing kinase-binding protein 1; SH3BP 1; SH3K1_HUMAN; SH3KBP1; Src family kinase binding protein 1; src related kinase binding protein 1
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-665
  • 氨基酸序列
    MVEAIVEFDY QAQHDDELTI SVGEIITNIR KEDGGWWEGQ INGRRGLFPD NFVREIKKEM KKDPLTNKAP EKPLHEVPSG NSLLSSETIL RTNKRGERRR RRCQVAFSYL PQNDDELELK VGDIIEVVGE VEEGWWEGVL NGKTGMFPSN FIKELSGESD ELGISQDEQL SKSSLRETTG SESDGGDSSS TKSEGANGTV ATAAIQPKKV KGVGFGDIFK DKPIKLRPRS IEVENDFLPV EKTIGKKLPA TTATPDSSKT EMDSRTKSKD YCKVIFPYEA QNDDELTIKE GDIVTLINKD CIDVGWWEGE LNGRRGVFPD NFVKLLPPDF EKEGNRPKKP PPPSAPVIKQ GAGTTERKHE IKKIPPERPE MLPNRTEEKE RPEREPKLDL QKPSVPAIPP KKPRPPKTNS LSRPGALPPR RPERPVGPLT HTRGDSPKID LAGSSLSGIL DKDLSDRSND IDLEGFDSVV SSTEKLSHPT TSRPKATGRR PPSQSLTSSS LSSPDIFDSP SPEEDKEEHI SLAHRGVDAS KKTSKTVTIS QVSDNKASLP PKPGTMAAGG GGPAPLSSAA PSPLSSSLGT AGHRANSPSL FGTEGKPKME PAASSQAAVE ELRTQVRELR SIIETMKDQQ KREIKQLLSE LDEEKKIRLR LQMEVNDIKK ALQSK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Adapter protein involved in regulating diverse signal transduction pathways. Involved in the regulation of endocytosis and lysosomal degradation of ligand-induced receptor tyrosine kinases, including EGFR and MET/hepatocyte growth factor receptor, through an association with CBL and endophilins. The association with CBL, and thus the receptor internalization, may be inhibited by an interaction with PDCD6IP and/or SPRY2. Involved in regulation of ligand-dependent endocytosis of the IgE receptor. Attenuates phosphatidylinositol 3-kinase activity by interaction with its regulatory subunit. May be involved in regulation of cell adhesion; promotes the interaction between TTK2B and PDCD6IP. May be involved in the regulation of cellular stress response via the MAPK pathways through its interaction with MAP3K4. Is involved in modulation of tumor necrosis factor mediated apoptosis. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape and migration. Has an essential role in the stimulation of B cell activation.
  • 基因功能参考文献：
    1. The multiple Src homology 3 (SH3) domains of trimeric CIN85 molecules associated with multiple SLP-65 molecules, which recruited further CIN85 trimers, thereby perpetuating the oligomerization process. PMID: 27353366
    2. these data support a role for the novel PP2Ac-CIN85 complex in supporting integrin-dependent platelet function by dampening the phosphatase activity. PMID: 27334924
    3. CIN85 promotes recycling of TGF-beta receptors and thereby positively regulates TGF-beta signaling. PMID: 26169354
    4. Results support the model that Cbl-CIN85-endophilin complex is not required for efficient internalization of EGFR, a prototype RTK. PMID: 25449262
    5. Multiple molecular forms of adaptor protein Ruk/CIN85 specifically associate with different subcellular compartments in human breast adenocarcinoma cell line. PMID: 25816594
    6. LOX-PP interacts with CIN85 via a novel SH3-binding motif and this association reduces CIN85-promoted invasion by breast cancer cells. PMID: 24167568
    7. Data show that EGFR activation leads to a pronounced src-mediated tyrosine phosphorylation of CIN85 that subsequently influences EGFR ubiquitination. PMID: 22833562
    8. this study indicates that high levels of Ruk(l)/CIN85 contribute to the conversion of breast adenocarcinoma cells into a more malignant phenotype via modulation of the Src/Akt pathway. PMID: 22791810
    9. Data show that CIN85 (c-Cbl interacting protein of 85 kDa) is constitutively associated with c-Cbl, Cbl-b, and B-cell linker in B cells. PMID: 22262777
    10. The CIN85 was constitutively associated with Hrs, and this strengthens the hypothesis of a functional role of CIN85 in endosomal EGFR sorting. PMID: 21635887
    11. the ubiquitination and degradation of stimulated FcgammaRIIa mediated by c-Cbl are positively regulated by the adaptor protein CIN85 in a PKC-dependent manner and these events contribute to the termination of FcgammaRIIa signaling. PMID: 21372129
    12. the N- and C-termini are important for the structure, stability, and function of the third SH3 domain of CIN85 PMID: 21446695
    13. ARAP1 associated with CIN85 affects epidermal growth factor receptor endocytic trafficking. PMID: 21275903
    14. High CIN85 is associated with the development and progression of head and neck squamous cell carcinoma. PMID: 20927317
    15. CIN85 binding to a C-terminal motif within hTTP leads to the increased phosphorylation of hTTP, possibly through enhanced association with MEKK4 PMID: 20221403
    16. Ruk/CIN85 induces PAI-1 expression via modulation of HIF-1alpha stability PMID: 20216986
    17. The ORF3 protein of hepatitis E virus was found to interact with CIN85 and compete with the formation of the growth factor receptor-Cbl-CIN85 complex, resulting in the reduced ubiquitination of CIN85. PMID: 20130058
    18. Cbl-CIN85-endophilin complex mediates ligand-induced downregulation of EGF receptors PMID: 11894095
    19. The endophilin-CIN85-Cbl complex mediates ligand-dependent downregulation of c-Met PMID: 11894096
    20. CIN85 participates in Cbl-b-mediated down-regulation of receptor tyrosine kinases PMID: 12177062
    21. Cbl-directed monoubiquitination of CIN85 is involved in regulation of ligand-induced degradation of EGF receptors. PMID: 12218189
    22. Cd2 antigen is linked to CAPZ via this protein and CMS. PMID: 12690097
    23. forms complexes with endonucleases in HEK293 cells PMID: 12946264
    24. CIN85 association with Dab2 is essential for its recruitment to clathrin coat and appears to be modulated by growth factor stimulation PMID: 14596919
    25. Data show that infected cell protein 0 (ICP0) binds CIN85 in a reciprocal manner and that the complexes pulled down by ICP0 also contain Cbl. PMID: 15824310
    26. CIN85 might play an important role in HSV-1 infection. PMID: 16223483
    27. Taken together, these results indicate a novel role for CIN85 in the regulation of cellular stress response via the MAPK pathways. PMID: 16256071
    28. CIN85, a Cbl-interacting protein, is a component of AMAP1-mediated breast cancer invasion machinery. PMID: 17255943
    29. a new role for CIN85 in regulating Syk protein levels in RBL-2H3 cells through the activation of the ubiquitin-proteasome pathway and provide a mechanism for this regulation involving c-Cbl ligase activity. PMID: 17675467
    30. These results suggest that endogenous Ruk/CIN85 may be involved in regulation of specific membrane trafficking processes. PMID: 18266907
    31. Results suggest that competition between Cbl and ubiquitin binding to CIN85 regulates Cbl function and EGFR endocytosis. PMID: 18680311
    32. Epidermal Growth Factor stimulation increases association of Cbl and Ruk/CIN85, which is dependent on tyrosine phosphorylation of the C-terminal region of Proto-Oncogene Proteins Cbl. PMID: 19166927
    33. detailed high-resolution solution structural study of CIN85A and CIN85B binding to proline-arginine peptides derived from the cognate ligands Cbl and Cbl-b PMID: 19268472
    34. CIN85 (SH3-domain kinase binding protein 1) associates with TNF receptor 1 via Src and modulates TNF-alpha-induced apoptosis. PMID: 15707590

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  • 亚细胞定位：
    Cytoplasm. Cytoplasm, cytoskeleton. Cytoplasmic vesicle membrane; Peripheral membrane protein. Cell junction, synapse, synaptosome. Cell junction, focal adhesion.
  • 组织特异性：
    Ubiquitously expressed. Also expressed in some cancer cell lines.
  • 数据库链接：

    HGNC: 13867

    OMIM: 300374

    KEGG: hsa:30011

    STRING: 9606.ENSP00000380921

    UniGene: Hs.726365