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  4. Recombinant Human Serine/threonine-protein kinase Nek3 (NEK3)

Recombinant Human Serine/threonine-protein kinase Nek3 (NEK3)

  • 中文名称：
    人NEK3重组蛋白
  • 货号：
    CSB-YP015701HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人NEK3重组蛋白
  • 货号：
    CSB-EP015701HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人NEK3重组蛋白
  • 货号：
    CSB-EP015701HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人NEK3重组蛋白
  • 货号：
    CSB-BP015701HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人NEK3重组蛋白
  • 货号：
    CSB-MP015701HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    NEK3
  • Uniprot No.：
  • 别名：
    NEK3; Serine/threonine-protein kinase Nek3; EC 2.7.11.1; HSPK 36; Never in mitosis A-related kinase 3; NimA-related protein kinase 3
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-506
  • 氨基酸序列
    MDDYMVLRMI GEGSFGRALL VQHESSNQMF AMKEIRLPKS FSNTQNSRKE AVLLAKMKHP NIVAFKESFE AEGHLYIVME YCDGGDLMQK IKQQKGKLFP EDMILNWFTQ MCLGVNHIHK KRVLHRDIKS KNIFLTQNGK VKLGDFGSAR LLSNPMAFAC TYVGTPYYVP PEIWENLPYN NKSDIWSLGC ILYELCTLKH PFQANSWKNL ILKVCQGCIS PLPSHYSYEL QFLVKQMFKR NPSHRPSATT LLSRGIVARL VQKCLPPEII MEYGEEVLEE IKNSKHNTPR KKTNPSRIRI ALGNEASTVQ EEEQDRKGSH TDLESINENL VESALRRVNR EEKGNKSVHL RKASSPNLHR RQWEKNVPNT ALTALENASI LTSSLTAEDD RGGSVIKYSK NTTRKQWLKE TPDTLLNILK NADLSLAFQT YTIYRPGSEG FLKGPLSEET EASDSVDGGH DSVILDPERL EPGLDEEDTD FEEEDDNPDW VSELKKRAGW QGLCDR
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Protein kinase which influences neuronal morphogenesis and polarity through effects on microtubules. Regulates microtubule acetylation in neurons. Contributes to prolactin-mediated phosphorylation of PXN and VAV2. Implicated in prolactin-mediated cytoskeletal reorganization and motility of breast cancer cells through mechanisms involving RAC1 activation and phosphorylation of PXN and VAV2.
  • 基因功能参考文献：
    1. NEK3 kinase phosphorylates SNAP29 on its serine 105. NEK3-mediated phosphorylation determines membrane localization of SNAP29. Membrane-associated SNAP29 regulates Golgi and focal adhesion structures. PMID: 29454964
    2. The data demonstrate that NEK3 is overexpressed in gastric cancer, which promotes the malignancy of gastric cancer PMID: 29504992
    3. these data support a modulatory role for phosphorylation at NEK3 Thr-165 in focal adhesion maturation and/or turnover to promote breast cancer cell migration. PMID: 27489110
    4. EHD2 interacts with Nek3 [NIMA (never in mitosis in Aspergillus nidulans)-related kinase 3], a serine/threonine kinase. PMID: 21756249
    5. Molecular cloning and characterization of the human NIMA-related protein kinase 3 gene (NEK3). PMID: 12063396
    6. Nek3 insertion/deletion polymorphism with alterations at 13q14 is associated with cancers PMID: 17118778
    7. Nek3 contributes to prolactin-mediated breast cancer motility through mechanisms involving Rac1 activation and paxillin phosphorylation. PMID: 17297458

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  • 亚细胞定位：
    Cytoplasm. Cell projection, axon.
  • 蛋白家族：
    Protein kinase superfamily, NEK Ser/Thr protein kinase family, NIMA subfamily
  • 组织特异性：
    Up-regulated in malignant versus normal breast tissue. Isoform 2 shows a high level of expression in testis, ovary and brain.
  • 数据库链接：

    HGNC: 7746

    OMIM: 604044

    KEGG: hsa:4752

    STRING: 9606.ENSP00000383210

    UniGene: Hs.409989