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  4. Recombinant Human Serine/threonine-protein kinase haspin (GSG2)

Recombinant Human Serine/threonine-protein kinase haspin (GSG2)

  • 中文名称：
    人HASPIN重组蛋白
  • 货号：
    CSB-YP819485HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人HASPIN重组蛋白
  • 货号：
    CSB-EP819485HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人HASPIN重组蛋白
  • 货号：
    CSB-EP819485HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人HASPIN重组蛋白
  • 货号：
    CSB-BP819485HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人HASPIN重组蛋白
  • 货号：
    CSB-MP819485HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    HASPIN
  • Uniprot No.：
  • 别名：
    germ cell associated 2; Germ cell-specific gene 2 protein; GSG2; H-haspin; Haploid germ cell-specific nuclear protein kinase; HASP_HUMAN; Serine/threonine-protein kinase haspin
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-798
  • 氨基酸序列
    MAASLPGPGS RLFRTYGAAD GRRQRRPGRE AAQWFPPQDR RRFFNSSGSS DASIGDPSQS DDPDDPDDPD FPGSPVRRRR RRPGGRVPKD RPSLTVTPKR WKLRARPSLT VTPRRLGLRA RPPQKCSTPC GPLRLPPFPS RDSGRLSPDL SVCGQPRDGD ELGISASLFS SLASPCPGSP TPRDSVISIG TSACLVAASA VPSGLHLPEV SLDRASLPCS QEEATGGAKD TRMVHQTRAS LRSVLFGLMN SGTPEDSEFR ADGKNMRESC CKRKLVVGNG PEGPGLSSTG KRRATGQDSC QERGLQEAVR REHQEASVPK GRIVPRGIDR LERTRSSRKS KHQEATETSL LHSHRFKKGQ KLGKDSFPTQ DLTPLQNVCF WTKTRASFSF HKKKIVTDVS EVCSIYTTAT SLSGSLLSEC SNRPVMNRTS GAPSSWHSSS MYLLSPLNTL SISNKKASDA EKVYGECSQK GPVPFSHCLP TEKLQRCEKI GEGVFGEVFQ TIADHTPVAI KIIAIEGPDL VNGSHQKTFE EILPEIIISK ELSLLSGEVC NRTEGFIGLN SVHCVQGSYP PLLLKAWDHY NSTKGSANDR PDFFKDDQLF IVLEFEFGGI DLEQMRTKLS SLATAKSILH QLTASLAVAE ASLRFEHRDL HWGNVLLKKT SLKKLHYTLN GKSSTIPSCG LQVSIIDYTL SRLERDGIVV FCDVSMDEDL FTGDGDYQFD IYRLMKKENN NRWGEYHPYS NVLWLHYLTD KMLKQMTFKT KCNTPAMKQI KRKIQEFHRT MLNFSSATDL LCQHSLFK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Serine/threonine-protein kinase that phosphorylates histone H3 at 'Thr-3' (H3T3ph) during mitosis. May act through H3T3ph to both position and modulate activation of AURKB and other components of the chromosomal passenger complex (CPC) at centromeres to ensure proper chromatid cohesion, metaphase alignment and normal progression through the cell cycle.
  • 基因功能参考文献：
    1. The mitotic histone kinase Haspin binds to the cohesin regulatory subunit Pds5B through a conserved YGA/R motif in its non-catalytic N terminus, which is similar to the recently reported YSR-motif-dependent binding of Wapl to Pds5B. PMID: 28343965
    2. The s' data identify the HIM of Pds5 as a binding motif for Haspin/Hrk1 in fission yeast. The s' analyses also show that human PDS5B binds Haspin through the same HIM-PIM interaction module, indicating that the Haspin localization mechanism is highly conserved. PMID: 28343969
    3. Autosomal recessive RP associated with mutations in the male germ cell-associated kinase (MAK) gene is associated with preservation of foveal vision even in advanced stages of retinal degeneration despite similar rates of peripheral visual field loss to other forms of autosomal recessive RP PMID: 29103961
    4. This is the first structural model of a bisubstrate inhibitor targeting haspin. The presented structural data represent a model for the future development of more specific haspin inhibitors PMID: 27139824
    5. Haspin kinase plays a key role in maintaining the slowly exchanging centromere Borealin pool. PMID: 25854549
    6. Haspin activity affects the phosphorylation state of proteins involved in gene expression regulation and splicing. PMID: 24732914
    7. Haspin inhibitors reveal centromeric roles of Aurora B in chromosome movement and spindle checkpoint signaling PMID: 23071152
    8. Aurora B phosphorylates Haspin to promote generation of H3T3ph and that Aurora B kinase activity is required for normal chromosomal localization of the CPC, indicating an intimate linkage between Aurora B and Haspin functions in mitosis PMID: 21658950
    9. findings show phosphorylation of histone H3-threonine 3 mediated by Haspin cooperates with Bub1-mediated histone 2A-serine 121 phosphorylation in targeting the chromosomal passenger complex to the inner centromere in fission yeast and human cells PMID: 20929775
    10. phosphorylation of H3T3 by Haspin is necessary for chromosomal passenger complex(CPC) accumulation at centromeres; Survivin binds to H3T3ph; H3T3ph generated by Haspin positions CPC at centromeres to regulate selected targets of Aurora B during mitosis PMID: 20705812
    11. Haspin-depleted cells reveal that spindle-pole integrity in mitosis requires chromosome cohesion. PMID: 19910498
    12. Haspin is a relatively newly discovered kinase that phosphorylates histone H3 during mitosis and appears to play a role in regulating chromosome behavior during cell division. [REVIEW] PMID: 19997740
    13. This work reveals a new kinase involved in composing the histone code and adds haspin to the select group of kinases that integrate regulation of chromosome and spindle function during mitosis and meiosis PMID: 15681610
    14. Haspin is required to maintain centromeric cohesion during mitosis. PMID: 17084365

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  • 亚细胞定位：
    Nucleus. Chromosome. Cytoplasm, cytoskeleton, spindle. Note=Nuclear during interphase and associates with the chromosomes and spindle apparatus during mitosis.
  • 蛋白家族：
    Protein kinase superfamily, Ser/Thr protein kinase family, Haspin subfamily
  • 组织特异性：
    Strongly expressed in testis. Also present in thymus and bone marrow and low levels observed in prostate, intestine, lung, spleen and lymph node. Expressed in fetal skin, liver, kidney and small intestine and also in proliferating but not non-proliferatin
  • 数据库链接：

    HGNC: 19682

    OMIM: 609240

    KEGG: hsa:83903

    STRING: 9606.ENSP00000325290

    UniGene: Hs.534059