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  4. Recombinant Human Single-minded homolog 2 (SIM2)

Recombinant Human Single-minded homolog 2 (SIM2)

  • 货号：
    CSB-YP621763HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP621763HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP621763HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP621763HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP621763HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    SIM2
  • Uniprot No.：
  • 别名：
    bHLHe15; Class E basic helix loop helix protein 15; Class E basic helix-loop-helix protein 15; MGC119447; SIM 2; SIM; Sim2; SIM2_HUMAN; Single minded homolog 2 (Drosophila); Single minded homolog 2; Single-minded homolog 2; Transcription factor SIM2
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-667
  • 氨基酸序列
    MKEKSKNAAK TRREKENGEF YELAKLLPLP SAITSQLDKA SIIRLTTSYL KMRAVFPEGL GDAWGQPSRA GPLDGVAKEL GSHLLQTLDG FVFVVASDGK IMYISETASV HLGLSQVELT GNSIYEYIHP SDHDEMTAVL TAHQPLHHHL LQEYEIERSF FLRMKCVLAK RNAGLTCSGY KVIHCSGYLK IRQYMLDMSL YDSCYQIVGL VAVGQSLPPS AITEIKLYSN MFMFRASLDL KLIFLDSRVT EVTGYEPQDL IEKTLYHHVH GCDVFHLRYA HHLLLVKGQV TTKYYRLLSK RGGWVWVQSY ATVVHNSRSS RPHCIVSVNY VLTEIEYKEL QLSLEQVSTA KSQDSWRTAL STSQETRKLV KPKNTKMKTK LRTNPYPPQQ YSSFQMDKLE CGQLGNWRAS PPASAAAPPE LQPHSESSDL LYTPSYSLPF SYHYGHFPLD SHVFSSKKPM LPAKFGQPQG SPCEVARFFL STLPASGECQ WHYANPLVPS SSSPAKNPPE PPANTARHSL VPSYEAPAAA VRRFGEDTAP PSFPSCGHYR EEPALGPAKA ARQAARDGAR LALARAAPEC CAPPTPEAPG APAQLPFVLL NYHRVLARRG PLGGAAPAAS GLACAPGGPE AATGALRLRH PSPAATSPPG APLPHYLGAS VIITNGR
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcription factor that may be a master gene of CNS development in cooperation with Arnt. It may have pleiotropic effects in the tissues expressed during development.
  • 基因功能参考文献：
    1. TMEM75 functions as an oncogene relying on activation of SIM2 in colorectal cancer. PMID: 29964097
    2. SIM2 increases CRT sensitivity through tumor differentiation by cooperation with ARNT. PMID: 29427302
    3. Two genes, OR51E2 and SIM2, and two miRNAs, miR-200c and miR-200b, showed significant association with prostate cancer. PMID: 28910345
    4. In SH-SY5Y cells, ethanol exposure increased Sim2 expression in a manner similar to that of cleaved caspase 3. PKA activation was required for this. Sim2 might be involved in activating caspase 3 and ethanol-induced apoptosis in SH-SY5Y cells. PMID: 25319570
    5. a single peptide containing multiple SIM2 epitopes can be used to induce both a CD4 and CD8 T cell response, providing a peptide-based vaccine formulation for potential use in immunotherapy of various cancers. PMID: 24690990
    6. Data showed that SIM2 was highly expressed in human glioma tumors and cell lines and its short form might increase the invasion of glioma cells through the alteration of epithelial-mesenchymal transition. PMID: 24909296
    7. miR-200a is downregulated in human glioma and inhibition of miR-200a caused upregulation of SIM2-s in T98G cells and promoted their motility. PMID: 24162743
    8. role for SIM2s in promoting human breast tumor differentiation and maintaining epithelial integrity. PMID: 22777354
    9. Altered expression of SIM2 and ETS2 could be one of the reasons for variable occurrence of different malignant conditions in Down syndrome. PMID: 23343470
    10. These results suggest an involvement of SIM2 in key traits of prostate tumor cell biology. PMID: 22174909
    11. Significantly lower frequency of SIM2 C-G haplotype (rs2073601-rs2073416) was noticed in individuals with Down syndrome (P value =0.01669) and their fathers (P value=0.01185) PMID: 22048266
    12. Genetic variants of SIM2 gene may not be associated with the susceptibility to congenital scoliosis and different clinical phenotypes of CS in Chinese Han population. PMID: 20137643
    13. The SIM2 is expressed in prostate cancer(PCa) and that anti-SIM2 antibodies are detectable in PCa patients' sera implicate SIM2 as a PCa-associated antigen that is a suitable potential target for PCa immunotherapy. PMID: 19737960
    14. Isoform-specific expression of SIM2 short-form (SIM2-s) was seen selectively in colon, prostate, and pancreatic carcinomas. PMID: 12676991
    15. a novel NXF signaling system on neural gene promoter may be a molecular target of the adverse effects of Sim2 in the mental retardation of Down's syndrome PMID: 14701734
    16. Potential role of SIM2 in central nervous system development. SIM2 overexpression could participate in pathogenesis of mental retardation in Down syndrome. PMID: 15946822
    17. provide a direct link between SIM2-s and differentiation, and may provide a model to identify SIM2-s targets PMID: 16129820
    18. Knockdown of SIM2s in MCF-7 breast cancer cells contributed to an epithelial-mesenchymal transition associated with increased MMP2 and slug levels. PMID: 18160708
    19. Ha-Ras transformation of MCF10A cells leads to repression of Singleminded-2s through NOTCH and C/EBPbeta. PMID: 19169276
    20. SIM2s' functional interference with HIF1alpha activities on BNIP3 may indicate a novel role for SIM2s in promoting tumourigenesis. PMID: 19668230
    21. The expression of a splice variant of SIM2, SIM2s, is lost or reduced in human breast cancers. Inhibits growth and invasiveness of breast cancer cells upon ectopic expression. Represses MMP3 expression by direct binding to the MMP3 promoter. PMID: 16840439
    22. Tissue- and tumor-specific expression of both SIM2 isoforms (SIM2-s and SIM2-l), but not SIM1, were detected in the pancreatic tumor models. PMID: 14550949
    23. Stage-specific expression of the sim2-short-form protein was seen in normal matched paraffin sections of the colon tumors. In a matched set of tissues of Benign Prostatic Hyperplasia (BPH) and prostate carcinomas, sim2-s expression was detected in the BPH PMID: 12530058

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  • 亚细胞定位：
    Nucleus.
  • 数据库链接：

    HGNC: 10883

    OMIM: 600892

    KEGG: hsa:6493

    STRING: 9606.ENSP00000290399

    UniGene: Hs.146186